Real time RT-PCR analyses of embryo-specific marker genes (, , , , , , and ) and in microspore cultures of non-embryogenic cv

Westar and the embryogenic Westar-derived DH-2 line. Stages of microspore-derived embryo (MDE) development (0 h, 1, 3, 5, and 7 d) are indicated for each of the lines (Westar, DH-2). Expression was calculated according to the 2 method (). Relative expression was based on comparisons with transcript levels in 0 h microspores of cv. Westar with 18S rRNA as the internal control for normalization.<p><b>Copyright information:</b></p><p>Taken from "Isolation of an embryogenic line from non-embryogenic cv. Westar through microspore embryogenesis"</p><p></p><p>Journal of Experimental Botany 2008;59(10):2857-2873.</p><p>Published online 13 Jun 2008</p><p>PMCID:PMC2486481.</p><p></p

Hormone profiling of metabolites and related compounds of ABA, auxin, and cytokinin in young and mature leaves and inflorescences (buds with 2–3 flowers) of

cv. Westar and Westar-derived DH-2. Histograms indicate mean values (±SE) for each of the measured compounds, in three replicate tissue samples, each harvested from a different set of plants.<p><b>Copyright information:</b></p><p>Taken from "Isolation of an embryogenic line from non-embryogenic cv. Westar through microspore embryogenesis"</p><p></p><p>Journal of Experimental Botany 2008;59(10):2857-2873.</p><p>Published online 13 Jun 2008</p><p>PMCID:PMC2486481.</p><p></p

Microarray analysis of differentially expressed genes between 7 d enlarged (dividing) embryogenic microspores of DH-2 and 7 d induced (non-dividing) microspores of the parental line, Westar

Labelled total RNA (10 μg) was used for hybridization to the Bn10K seed cDNA array. Signal intensities were normalized and gene lists extracted using SAM (minimum 1.5-fold change in expression). A total of 117 differentially expressed genes were identified: 77 genes up-regulated in DH-2 and 40 genes up-regulated in Westar (negative log2 values). Gene identifications are listed in and .<p><b>Copyright information:</b></p><p>Taken from "Isolation of an embryogenic line from non-embryogenic cv. Westar through microspore embryogenesis"</p><p></p><p>Journal of Experimental Botany 2008;59(10):2857-2873.</p><p>Published online 13 Jun 2008</p><p>PMCID:PMC2486481.</p><p></p

Real time RT-PCR analyses of pollen-specific genes (At3g18810), (At2g31500), (At4g13230), (At2g24370), and in microspore cultures of non-embryogenic cv

Westar and the embryogenic Westar-derived DH-2 line (the closest match for each pollen-specific gene is given in parentheses). Stages of microspore-derived embryo (MDE) development (0 h, 1, 3, 5, and 7 d) are indicated for each of the lines (Westar, DH-2). Expression was calculated according to the 2 method (). Relative expression was based on comparisons with transcript levels in 0 h microspores of cv. Westar with 18S rRNA as the internal control for normalization.<p><b>Copyright information:</b></p><p>Taken from "Isolation of an embryogenic line from non-embryogenic cv. Westar through microspore embryogenesis"</p><p></p><p>Journal of Experimental Botany 2008;59(10):2857-2873.</p><p>Published online 13 Jun 2008</p><p>PMCID:PMC2486481.</p><p></p