Clinical characteristics and laboratory values of children on admission, and outcome

†<p>Date of vaccination unless otherwise stated.</p>‡<p>HBV: Hepatitis B virus. Both received Euvax B™; MMR: Measles, Mumps, Rubella. All received Priorix™; varicella: Varilrix^R.</p>§<p>swelling at injection site beyond the expected after routine vaccination.</p>∥<p>Inotropes used were dopamine, dobutamine, noradrenaline.</p>**<p>MRSA: methicillin-resistant <i>Staphylococcus aureus</i>. NA denotes not available, a plus sign positive or present, and a minus sign negative or absent.</p>*<p>Normal ranges are as follows: hemoglobin concentration, 105–135 g/L; leukocyte count, 6 to 17.5·10⁹/L; platelet count, 150 to 400·10⁹/L; alanine aminotransferase (ALT) level, below 37 U/L; aspartate aminotransferase (AST) level, below 40 U/L; creatine phosphokinase (CK) below 200 U/L; serum creatinine concentration, 18–80 µmol/L;</p

Rapidly progressive soft tissue infection after vaccination.

<p>Severe tissue necrosis around vaccination site in Child 4, two days following vaccination with MMR. MRSA was cultured from wound fluid.</p

Key virulence and resistance genes in isolates HCM3A, HT2001 751, and MW2 as detected by microarray.

*<p>Confirmed with PCR and/or sequencing</p>**<p>Microarray data cannot conclusively prove the location of a gene. Assignation to a MGE is based on known location of that gene in other sequenced strains.</p><p><i>hla</i>, α-haemolysin; <i>hlgACB</i>, γ-haemolysin; <i>hld</i>, δ-haemolysin; <i>clf</i>, clumping factors; ST, sequence type using MLST; <i>cap8</i>, capsule type 8; <i>sasG</i>, accumulation associated protein SA2285; <i>coa</i> M0206v, coagulase with four types described; <i>sasA</i>, haemagglutinin like gene which can contain an insert; <i>cna</i>, collagen binding protein; <i>sdr</i>, serine aspartate repeat proteins; <i>fnbpA</i>, fibronectin binding protein with three types described; <i>lytN</i>, cell wall hydrolase; <i>agr</i>, accessory gene regulator with 4 classes described; <i>trap</i>, target of RNAIII activating protein with 2 types described; <i>sarT</i>, staphylococcal accessory regulator T; MGE, mobile genetic element; SCC, staphylococcal cassette chromosome; <i>mec</i>, methicillin resistance; <i>ccr</i>, cassette chromosome recombinase with 3 types described; <i>far1</i>, putative fusidic acid resistance; <i>PV-luk</i>, Panton Valentine leukocidin; <i>seg</i>, enterotoxin G; <i>sak</i>, staphylokinase; <i>sea</i>, enterotoxin A; <i>sek</i>, enterotoxin K; <i>seb</i>, enterotoxin B; <i>sec</i>, enterotoxin C; <i>bla</i>, β-lactamase; <i>cad</i>, cadmium resistance; <i>ars</i>, arsenic resistance; <i>bacteriocin</i>, putative bacteriocin gene SAR0694-5; - denotes gene not detected; #, phage unrelated to PV-<i>luk</i> phage in MW2.</p