Coherent neutral pion photoproduction on the deuteron

The differential scattering cross section for the process gd→dp0 was measured, as part of experiment E89-012 at Thomas Jefferson National Accelerator Facility. The experiment was performed in Hall C during the Spring of 1996 as the commissioning experiment for the Hall C cryogenic target. The High Momentum Spectrometer was used to detect the recoil deuteron and no effort was made to detect the p0 or its decay photons. The differential cross section was measured at a number of incident photon energies between 0.8 GeV and 4.0 GeV for the deuteron center-of-mass angles of 90?? and 136??. The data were found to disagree with both the constituent counting rule and reduced nuclear amplitude predictions. These are the first data at large deuteron center-of-mass angles for photon energies larger than 1.6 GeV

Mechanistic Insights into Glucan Phosphatase Activity against Polyglucan Substrates

Glucan phosphatases are central to the regulation of starch and glycogen metabolism. Plants contain two known glucan phosphatases, Starch EXcess4 (SEX4) and Like Sex Four2 (LSF2), which dephosphorylate starch. Starch is water-insoluble and reversible phosphorylation solubilizes its outer surface allowing processive degradation. Vertebrates contain a single known glucan phosphatase, laforin, that dephosphorylates glycogen. In the absence of laforin, water-soluble glycogen becomes insoluble, leading to the neurodegenerative disorder Lafora Disease. Because of their essential role in starch and glycogen metabolism glucan phosphatases are of significant interest, yet a comparative analysis of their activities against diverse glucan substrates has not been established. We identify active site residues required for specific glucan dephosphorylation, defining a glucan phosphatase signature motif (CζAGΨGR) in the active site loop. We further explore the basis for phosphate position-specific activity of these enzymes and determine that their diverse phosphate position-specific activity is governed by the phosphatase domain. In addition, we find key differences in glucan phosphatase activity toward soluble and insoluble polyglucan substrates, resulting from the participation of ancillary glucan-binding domains. Together, these data provide fundamental insights into the specific activity of glucan phosphatases against diverse polyglucan substrates

Structural Mechanism of Laforin Function in Glycogen Dephosphorylation and Lafora Disease

Glycogen is the major mammalian glucose storage cache and is critical for energy homeostasis. Glycogen synthesis in neurons must be tightly controlled due to neuronal sensitivity to perturbations in glycogen metabolism. Lafora disease (LD) is a fatal, congenital, neurodegenerative epilepsy. Mutations in the gene encoding the glycogen phosphatase laforin result in hyperphosphorylated glycogen that forms water-insoluble inclusions called Lafora bodies (LBs). LBs induce neuronal apoptosis and are the causative agent of LD. The mechanism of glycogen dephosphorylation by laforin and dysfunction in LD is unknown. We report the crystal structure of laforin bound to phosphoglucan product, revealing its unique integrated tertiary and quaternary structure. Structure-guided mutagenesis combined with biophysical and biochemical analyses reveal the basis for normal function of laforin in glycogen metabolism. Analyses of LD patient mutations define the mechanism by which subsets of mutations disrupt laforin function. These data provide fundamental insights connecting glycogen metabolism to neurodegenerative disease

Separation of the Longitudinal and Transverse Cross Sections in the p(ee'K)Lambda and p(ee'K)Sigma Reactions

We report measurements of cross sections for the reaction p(e,e'K)Y, for both the Lambda and Sigma_0 hyperon states, at an invariant mass of W=1.84 GeV and four-momentum transfers 0.5<Q2<2 (GeV/c)2. Data were taken for three values of virtual photon polarization, allowing the decomposition of the cross sections into longitudinal and transverse components. The Lambda data is a revised analysis of prior work, whereas the Sigma_0 results have not been previously reported.Comment: 17 pages, 18 figures, REVTEX 4, submitted to Physical Review

Phenomenology of the deuteron electromagnetic form factors

A rigorous extraction of the deuteron charge form factors from tensor polarization data in elastic electron-deuteron scattering, at given values of the 4-momentum transfer, is presented. Then the world data for elastic electron-deuteron scattering is used to parameterize, in three different ways, the three electromagnetic form factors of the deuteron in the 4-momentum transfer range 0-7 fm. This procedure is made possible with the advent of recent polarization measurements. The parameterizations allow a phenomenological characterization of the deuteron electromagnetic structure. They can be used to remove ambiguities in the form factors extraction from future polarization data