6 research outputs found
Monitoring DNA Hybridization and Thermal Dissociation at the Silica/Water Interface Using Resonantly Enhanced Second Harmonic Generation Spectroscopy
The immobilization of oligonucleotide
sequences onto glass supports
is central to the field of biodiagnostics and molecular biology with
the widespread use of DNA microarrays. However, the influence of confinement
on the behavior of DNA immobilized on silica is not well understood
owing to the difficulties associated with monitoring this buried interface.
Second harmonic generation (SHG) is an inherently surface specific
technique making it well suited to observe processes at insulator
interfaces like silica. Using a universal 3-nitropyrolle nucleotide
as an SHG-active label, we monitored the hybridization rate and thermal
dissociation of a 15-mer of DNA immobilized at the silica/aqueous
interface. The immobilized DNA exhibits hybridization rates on the
minute time scale, which is much slower than hybridization kinetics
in solution but on par with hybridization behavior observed at electrochemical
interfaces. In contrast, the thermal dissociation temperature of the
DNA immobilized on silica is on average 12 °C lower than the
analogous duplex in solution, which is more significant than that
observed on other surfaces like gold. We attribute the destabilizing
affect of silica to its negatively charged surface at neutral pH that
repels the hybridizing complementary DNA
Specific Cation Effects on the Bimodal Acid–Base Behavior of the Silica/Water Interface
Using nonresonant second harmonic generation spectroscopy,
we have
monitored the change in surface charge density of the silica/water
interface over a broad pH range in the presence of different alkali
chlorides. Planar silica is known to possess two types of surface
sites with p<i>K</i><sub>a</sub> values of ∼4 and
∼9, which are attributed to different solvation environments
of the silanols. We report that varying the alkali chloride electrolyte
significantly changes the effective acid dissociation constant (p<i>K</i><sub>a</sub><sup>eff</sup>) for the less acidic silanol
groups, with the silica/NaCl<sub>aq</sub> and silica/CsCl<sub>aq</sub> interfaces exhibiting the lowest and highest p<i>K</i><sub>a</sub><sup>eff</sup> values of 8.3(1) and 10.8(1), respectively.
Additionally, the relative populations of the two silanol groups are
also very sensitive to the electrolyte identity. The greatest percentage
of acidic silanol groups was 60(2)% for the silica/LiCl<sub>aq</sub> interface in contrast to the lowest value of 20(2)% for the silica/NaCl<sub>aq</sub> interface. We attribute these changes in the bimodal behavior
to the influence of alkali ions on the interfacial water structure
and its corresponding effect on surface acidity
Halide-Induced Cooperative Acid–Base Behavior at a Negatively Charged Interface
Using
second harmonic generation and sum frequency generation spectroscopy,
we monitor the influence of sodium and potassium halides on acid–base
processes at the negatively charged silica/aqueous electrolyte interface.
We find that the two types of acidic silanols at the surface are very
sensitive to the presence of halides in the aqueous phase. As the
halide size increases, the pH at which half the more acidic sites
are deprotonated (pH<sub>0.5</sub>) shifts to lower pH. Conversely,
the pH<sub>0.5</sub> of the less acidic sites shifts to higher pH
with increasing halide size. We also observe titration curves of increasing
sharpness as the halide size increases, indicative of positive cooperativity.
Using a simple cooperative model, we find that the cooperative unit
for the dissociation of more acidic surface sites is ∼1, 2,
and 3 for the chloride, bromide, and iodide electrolytes, respectively,
which reveals that the larger anions promote deprotonation among the
more acidic silanol groups. We also find that the fraction of more
acidic sites, proportional to the relative surface charge density
at neutral pH, increases from 20% to 86% as the sodium halide is varied
from chloride to iodide. As the percentage of more acidic sites and
the surface charge at neutral pH increases, the effective acidity
of the less acidic sites decreases, indicating that greater surface
charge density renders the remaining silanol groups more difficult
to deprotonate. As the relative amount of less acidic sites increases,
their deprotonation events exhibit negative, rather than positive,
cooperativity revealing charge repulsion between neighboring silanol
groups
Mussel-Inspired Immobilization of Silver Nanoparticles toward Antimicrobial Cellulose Paper
Paper
and paper products are widely used without any antimicrobial
efficacy in our everyday lives and thus can act as potential transporters
of many diseases. Herein, we introduce antimicrobial activity to cellulose
paper by presenting a tailored mussel-inspired strategy for the sustainable
immobilization of silver nanoparticles (AgNPs), which are well-known
for the effectiveness in preventing annexation and proliferation of
microbes on materials surfaces. First, we functionalized the cellulose
paper with succinic acid that eventually reacted with dopamine to
give dopamine-modified paper. The dopamine molecules possess excellent
adhesion and strong coordination with metal substrates through catechol
groups offering a potentially robust interface between AgNPs and the
organic structure of the paper. Next, AgNPs were deposited onto the
paper by simply immersing dopamine-modified paper in a silver salt
solution to accomplish the antimicrobial properties. Field emission
scanning electron microscopic study of the synthesized antimicrobial
papers confirmed that the loading of AgNPs was time-dependent, and
the average size of the nanoparticles was in the range of 50–60
nm after 8 h of deposition time. The paper decorated with AgNPs showed
excellent antimicrobial activity against highly virulent and multiple
antibiotic resistant Gram-positive and Gram-negative pathogenic bacteria
as well as against some extremely virulent fungal phytopathogens
pH-Dependent Inversion of Hofmeister Trends in the Water Structure of the Electrical Double Layer
Specific ion effects
(SIEs) are known to influence the acid/base
behavior of silica and the interfacial structure of water, yet evidence
of the effect of pH on SIEs is lacking. Here broadband vibrational
sum frequency generation (SFG) spectroscopy was used to study SIEs
on the water structure at the electrical double layer (EDL) of silica
as a function of pH and monovalent cation identity from pH 2–12
at 0.5 M salt concentration. SFG results indicate a direct Hofmeister
series of cation adsorption at pH 8 (Li<sup>+</sup> < Na<sup>+</sup> < K<sup>+</sup> < Cs<sup>+</sup>), with an inversion in this
series occurring at pH > 10. In addition, an inversion in SFG intensity
trends also occurred at pH < 6, which was attributed to contributions
from asymmetric cation hydration and EDL overcharging. The highly
pH-dependent SIEs for silica/water have implications for EDL models
that often assume pH-independent parameters
Separating the pH-Dependent Behavior of Water in the Stern and Diffuse Layers with Varying Salt Concentration
Vibrational sum frequency
generation (SFG) spectroscopy was utilized
to distinguish different populations of water molecules within the
electric double layer (EDL) at the silica/water interface. By systematically
varying the electrolyte concentration, surface deprotonation, and
SFG polarization combinations, we provide evidence of two regions
of water molecules that have distinct pH-dependent behavior when the
Stern layer is present (with onset between 10 and 100 mM NaCl). For
example, water molecules near the surface in the Stern layer can be
probed by the pss polarization combination, while other polarization
combinations (ssp and ppp) predominantly probe water molecules further
from the surface in the diffuse part of the electrical double layer.
For the water molecules adjacent to the surface within the Stern layer,
upon increasing the pH from the point-of-zero charge of silica (pH
∼2) to higher values (pH ∼12), we observe an increase
in alignment consistent with a more negative surface with increasing
pH. In contrast, water molecules further from the surface appear to
exhibit a net flip in orientation upon increasing the pH over the
same range, which we attribute to the presence of the Stern layer
and possible overcharging of the EDL at lower pH. The opposing pH-dependent
behavior of water in these two regions sheds new light on our understanding
of the water structure within the EDL at high salt concentrations
when the Stern layer is present