Effect of organic and inorganic fertilizer on the growth and yield components of traditional and improved rice (Oryza sativa L.) genotypes in Malaysia

Rice is the most important staple cereal human nutrition and consumed by 75% of the global population. Rice plants need a supply of essential nutrients for their optimal growth. Rice production has increased tremendously in Malaysia insensitive irrigation and the use of inorganic fertilizers and pesticides. However, the effect of using inorganic fertilizers resulted in contamination of ground water and decreased the productivity of soil, which in turn affected the rice production in the long term. The use of organic manure may help to regain the soil health, but that is insufficient for providing the essential nutrients to achieve optimal growth. Therefore, the use of organic manure combined with inorganic fertilizers is applied to obtain optimum yields. This study aims to test the effect of organic and inorganic fertilizers on the growth and yield components of 65 rice genotypes. The pot experiment was conducted at the net house on field 10, University Putra Malaysia, UPM, Malaysia, during the period of February to June 2019 and August to December 2019 in a randomized complete block design (RCBD) with three replications. There were three treatment combinations viz. T1: 5 t ha−1 chicken manure (CM), T2: 2.5 t ha−1 CM + 50% CFRR, T3: 100% (150 N: 60 P2O5: 60 K2O kg ha−1) and chemical fertilizer recommended rate (CFRR). Grain and straw samples were collected for chemical analysis, and physical parameters were measured at the harvest stage. Results showed that most of the growth and yield components were significantly influenced due to the application of organic manure with chemical fertilizer. The application of chemical fertilizer alone or in combination with organic manure resulted in a significant increase in growth, yield component traits, and nutrient content (N, P, and K) of all rice genotypes. Treatment of 2.5 t ha−1 CM + 50% CFRR as well as 100% CFRR showed a better performance than the other treatments. It was observed that the yield of rice genotypes can be increased substantially with the judicious application of organic manure with chemical fertilizer. The benefits of the mixed fertilization (organic + inorganic) were not only the crop yields but also the promotion of soil health, the reduction of chemical fertilizer input, etc

Half diallel analysis for biochemical and morphological traits in cultivated eggplants (Solanum melongena L.)

Eleven morphologically diverse cultivated eggplant accessions were used for hybridization following half diallel mating design to obtain 55 hybrids. Evaluation of hybrids along with the parents was conducted over two locations followed by randomised complete block design with three replications to study gene action and combining ability of 15 morphological and biochemical traits. The analysis of variance indicated highly significant differences among the environments and interaction of genotype and environment, except for fruit length to width ratio. Additive gene effects were significant for the inheritance of these traits and expression of these additive genes were greatly affected by environments. The general combining ability (GCA) was greater than their respective specific combining ability (SCA) for all traits except for fruit yield per plant. High values of GCA and SCA effects for characters of interest were dispersed among different genotypes. From this study it was observed that the best parental line was BT15 based on days to first flowering, total number of fruits per plant, total soluble solids and total phenol content. Besides, the parent BM5 showed good general combining ability effects for fruit yield per plant, fruit length and fruit length to width ratio and the parent BB1 performed good general combining ability for fruit diameter, fruit girth and fruit weight. Besides, other parents showed the best performance for only one trait. On the other hand, the hybrid BT6 × BT15 was reported bearing early flowering with high total phenol content and the hybrid BM9 × BB26 has high fruit yield with high soluble solids. Besides, the hybrid BM9 × BB1 has a high fruit diameter and fruit weight. All other hybrids except for these three (BT6 × BT15, BM9 × BB26 and BM9 × BB1) were shown the best performance for only one trait. Hence, based on the desired trait, the hybrid can be selected for future use after large scale evaluation

Recent Advances in Rice Varietal Development for Durable Resistance to Biotic and Abiotic Stresses through Marker-Assisted Gene Pyramiding

Abiotic and biotic stresses adversely affect rice growth, development and grain yield. Traditional rice breeding techniques are insufficient in modern agriculture to meet the growing population’s food needs on a long-term basis. The development of DNA markers closely linked to target genes or QTLs on rice chromosomes, and advanced molecular techniques, such as marker-assisted selection (MAS), have encouraged the evolution of contemporary techniques in rice genetics and breeding, such as gene pyramiding. Gene pyramiding refers to the act of combining two or more genes from multiple parents into a single genotype, which allows the overexpression of more than one gene for broad-spectrum abiotic and biotic stress resistance. Marker-assisted pedigree, backcrossing and pseudo-backcrossing methods can increase the conventional breeding speed by reducing the number of breeding generations in order to enhance the pyramiding process. Pyramiding is affected by several factors: the number of transferred genes; the range within gene and flanking markers; the number of chosen populations in every breeding generation; the features of genes and germplasms; and the potentiality of breeders to identify the target genes. Modern breeding methods, such as the marker-assisted backcrossing approach, have made gene pyramiding more precise and reliable for the development of stress-tolerant rice varieties in the coming decades. This review presents up-to-date knowledge on gene pyramiding schemes, marker-assisted gene pyramiding techniques, the efficiency of marker-assisted gene pyramiding and the advantages and limitations of gene pyramiding methods. This review also reports on the potential application of marker-assisted selection breeding to develop stress-tolerant rice varieties that stabilize abiotic and biotic stresses. This review will help rice breeders to improve yields by increasing rice productivity under abiotic and biotic stress conditions

The Nutrient Content, Growth, Yield, and Yield Attribute Traits of Rice (Oryza sativa L.) Genotypes as Influenced by Organic Fertilizer in Malaysia

One of the most important challenges to continuously maximizing crop production on limited areas of agricultural land is maintaining or enhancing soil fertility. Organic fertilizer application is needed to replace nutrients recovered by crops from the fields in order to restore the crop production potential of the soil. The utilization of chicken manure as an organic fertilizer is essential in improving soil productivity and cop production. In Malaysia, demand for rice as a food source is rising in tandem with population growth, while paddy rice production capacity is becoming increasingly constrained. Field experiments were carried out in Sungai besar, Kuala Selangor, Malaysia during the two planting seasons in 2020 to evaluate the effects of different levels of organic fertilizer on the growth and yield of rice genotypes. A split plot layout in a randomized complete block design with three replicates was used. The twelve rice genotypes were in the main plots. The sub-plots were treatments. The experiment comprised 4 treatments, viz., T1 = 100% NPK (N150P60K60), T2 = Chicken manure @ 5 t ha−1, T3 = Chicken manure @ 7 t ha−1, and T4 = Chicken manure @ 10 t ha−1. The study indicated that different levels of chicken manure and NPK fertilizer showed significant effects on growth, yield, and yield contributing characters of all the rice genotypes. Results showed that application of chicken manure 10 t ha-1 was the best in producing growth and yield contributing characters, grain and straw yields, and also nutrient (N, P, and K) contents in grain and straw. The maximum number of panicles (422.56 panicles m−1), the maximum number of filled grains (224.49 grains panicle−1), and the maximum grain yield (8.02 t ha−1) and straw yield (9.88 t ha−1) were recorded from T4 treatment at the rice genotype BRRI dhan75. Although the highest biological yield was recorded from T4 treatment, a statistically similar result was found for T3 treatment. The highest harvest index was also recorded for T4 treatment. Therefore, rice genotype BRRI dhan75 can be recommended under chicken manure @ 10 t ha−1 for rice production in Malaysia

The nutrient content, growth, yield, and yield attribute traits of rice (Oryza sativa L.) genotypes as influenced by organic fertilizer in Malaysia

One of the most important challenges to continuously maximizing crop production on limited areas of agricultural land is maintaining or enhancing soil fertility. Organic fertilizer application is needed to replace nutrients recovered by crops from the fields in order to restore the crop production potential of the soil. The utilization of chicken manure as an organic fertilizer is essential in improving soil productivity and cop production. In Malaysia, demand for rice as a food source is rising in tandem with population growth, while paddy rice production capacity is becoming increasingly constrained. Field experiments were carried out in Sungai besar, Kuala Selangor, Malaysia during the two planting seasons in 2020 to evaluate the effects of different levels of organic fertilizer on the growth and yield of rice genotypes. A split plot layout in a randomized complete block design with three replicates was used. The twelve rice genotypes were in the main plots. The sub-plots were treatments. The experiment comprised 4 treatments, viz., T1 = 100% NPK (N150P60K60), T2 = Chicken manure @ 5 t ha−1, T3 = Chicken manure @ 7 t ha−1, and T4 = Chicken manure @ 10 t ha−1. The study indicated that different levels of chicken manure and NPK fertilizer showed significant effects on growth, yield, and yield contributing characters of all the rice genotypes. Results showed that application of chicken manure 10 t ha-1 was the best in producing growth and yield contributing characters, grain and straw yields, and also nutrient (N, P, and K) contents in grain and straw. The maximum number of panicles (422.56 panicles m−1), the maximum number of filled grains (224.49 grains panicle−1), and the maximum grain yield (8.02 t ha−1) and straw yield (9.88 t ha−1) were recorded from T4 treatment at the rice genotype BRRI dhan75. Although the highest biological yield was recorded from T4 treatment, a statistically similar result was found for T3 treatment. The highest harvest index was also recorded for T4 treatment. Therefore, rice genotype BRRI dhan75 can be recommended under chicken manure @ 10 t ha−1 for rice production in Malaysia

Advanced breeding strategies and future perspectives of salinity tolerance in rice

Rice, generally classified as a typical glycophyte, often faces abiotic stresses such as excessive drought, high salinity, prolonged submergence, cold, and temperature, which significantly affects growth, development, and ultimately, grain yield. Among these negative impacts of abiotic factors in rice production, salinity stress is a major constraint, followed by drought. There is considerable research on the use of marker-assisted selection (MAS), genome editing techniques, and transgenic studies that have profoundly improved the present-day rice breeders’ toolboxes for developing salt-tolerant varieties. Salinity stresses significantly affect rice plants during seedling and reproductive stages. Hence, greater understanding and manipulation of genetic architecture in developing salt-tolerant rice varieties will significantly impact sustainable rice production. Rice plants’ susceptibility or tolerance to high salinity has been reported to be the result of coordinated actions of multiple stress-responsive quantitative trait loci (QTLs)/genes. This paper reviews recent literature, updating the effects of salinity stress on rice plants and germplasm collections and screening for salinity tolerance by different breeding techniques. Mapping and identification of QTLs salt tolerance genes are illuminated. The present review updates recent breeding for improvement in rice tolerance to salinity stress and how state-of-the-art tools such as MAS or genetic engineering and genome editing techniques, including mutagenesis and conventional breeding techniques, can assist in transferring salt-tolerant QTLs genes into elite rice genotypes, accelerating breeding of salt-resistant rice cultivars

Genetic diversity analysis among Capsicum annuum mutants based on morpho-physiological and yield traits

It is crucial to assess genetically superior parents when developing novel hybrids. This experiment was conducted to find out the diversity of 27 Capsicum annuum mutant lines derived from two varieties.To achieve the objective, 23 morpho-physiological and yield traits were recorded through two planting seasons. Highly significant differences (p < 0.01) were recorded among the studied traits. There was a strong to moderately positive phenotypic association between yield and all other morphological traits except first bifurcation length, stem diameter, pedicle length, flowering date, and maturity date. A higher Genotypic Coefficient of Variation (GCV) and Phenotypic Coefficient of Variation (PCV), combined with moderate to high heritability and high hereditary progress, have been found in the number of fruits per plant, fruit yield per plant, and number of seeds per fruit. High heritability was found in yield characteristics, vis-à-visnumber of seeds per fruit, number of fruits per plant, and indicated high genetic advance. The studied genotypes were divided into six groups after the cluster analysis. Based on the correlation matrix of 23 quantitative characteristics, principal component analysis revealed that the percentage of variation for PC1 and PC2 is 28%and 19%, respectively, andPC1 represents the largest percentage of the overall total variation. The calculated genetic distance also explains the potential of heterosis breeding. The revealed findings might be helpful for breeders to target quantitative characters and the parental lines of C. annuum during the execution of their future breeding programmes for developing high-yielding and climate-resilient chilli varieties

Genetic Diversity Analysis among Capsicum annuum Mutants Based on Morpho-Physiological and Yield Traits

It is crucial to assess genetically superior parents when developing novel hybrids. This experiment was conducted to find out the diversity of 27 Capsicum annuum mutant lines derived from two varieties.To achieve the objective, 23 morpho-physiological and yield traits were recorded through two planting seasons. Highly significant differences (p &lt; 0.01) were recorded among the studied traits. There was a strong to moderately positive phenotypic association between yield and all other morphological traits except first bifurcation length, stem diameter, pedicle length, flowering date, and maturity date. A higher Genotypic Coefficient of Variation (GCV) and Phenotypic Coefficient of Variation (PCV), combined with moderate to high heritability and high hereditary progress, have been found in the number of fruits per plant, fruit yield per plant, and number of seeds per fruit. High heritability was found in yield characteristics, vis-&agrave;-visnumber of seeds per fruit, number of fruits per plant, and indicated high genetic advance. The studied genotypes were divided into six groups after the cluster analysis. Based on the correlation matrix of 23 quantitative characteristics, principal component analysis revealed that the percentage of variation for PC1 and PC2 is 28%and 19%, respectively, andPC1 represents the largest percentage of the overall total variation. The calculated genetic distance also explains the potential of heterosis breeding. The revealed findings might be helpful for breeders to target quantitative characters and the parental lines of C. annuum during the execution of their future breeding programmes for developing high-yielding and climate-resilient chilli varieties