9 research outputs found
PZ-peptidase activity from ejaculated boar spermatozoa
No full textProtein constituents of the boar spermatozoon were fractionated in three components, the hypotonic soluble fraction, the detergent-soluble fraction, and the detergent-insoluble fraction. When all these fractions were assayed spectrophotometrically using the PZ-peptide as substrate, a high value of PZ-peptidase specific activity was observed in the first fraction. Electrophoretic analysis at pH 8.3 of the protein content from the hypotonic soluble fraction revealed the existence of multiple molecular forms capable of hydrolysing the PZ-peptide. The major form was characterized by a surprisingly high value of electrophoretic mobility, index of the presence of numerous negatively charged residues. Biochemical and ultrastructural analyses showed that the hypotonic soluble fraction did not contain intrinsic, and specifically acrosomal, sperm enzymes
Differentiation between restrictive cardiomyopathy and constrictive pericarditis by the measurement of doppler myocardial imaging derived myocardial velocity gradient
No full textAssignment of the porcine acrosin gene, ACR, to Chromosome 5p15 by fluorescence in situ hybridization (FISH)
No full textA contribution to the application of the plant-growth regulator chlorocholine chloride (CCC) in vegetable crops 2. Communication: Biochemical studies in the metabolism of CCC in kohlrabi, cauliflower, and tomatoes
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