Adaptive response to oxidative stress in the filamentous fungus Aspergillus niger B1-D

In the present study, we used a recombinant filamentous fungus strain, Aspergillus niger B1-D, as a model system, and investigated the antioxidant defences in this organism. Our findings indicate that pretreatment with low concentrations of H2O2 completely prevents killing by this oxidant at high concentrations. It shows that A. niger adapts to exposure to H2O2 by reducing growth and inducing a number of antioxidant enzyme activities, including superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, of which the induction of catalase is the most pronounced. Moreover the decline of these antioxidant enzymes activities after H2O2 detoxification, coincides with recommencement of growth. Results from monitoring the extracellular H2O2 concentration clearly indicate a very rapid detoxification rate for H2O2 in adapted A. niger cultures. A mathematical model predicts only very low concentrations of intracellular H2O2 accumulating in such cultures. Our results also show that glutathione plays a role in the oxidative defence against H2O2 in A. niger. On addition of H2O2, the intracellular pool of glutathione increases while the redox state of glutathione becomes more oxidized

The College Football Gambling Market An Empirical Approach

This study tests the efficiency of the college football gambling market and whether the market allows for profitable wagering. Operating upon the theoretical framework that, at any given time, prices fully reflect all information available in a particular market, I test for the existence of residual information that is not currently incorporated into the market, thus rendering it inefficient. This project expands upon several previous studies performed on sports betting – most notably that of Zuber, Gandar, and Bowers (1985), which examined the gambling market efficiency for National Football League games. The findings prove to be consistent with the conclusions reached in these prior analyses, which suggest that speculative inefficiencies exist within the market

Humanitarian Intervention Before YouTube

Histor

Summer for the Gods: The Scopes Trial and America’s Continuing Debate Over Science and Religion by Edward L. Larson (2006)

Histor

An unaveraged computational model of a variably polarised undulator FEL

An un­aver­aged 3D model of the FEL has been de­vel­oped which can model vari­ably po­larised un­du­la­tors. The ra­di­a­tion field po­lar­i­sa­tion is self-con­sis­tent­ly driv­en by the elec­tron dy­nam­ics and is com­plete­ly vari­able. This paper de­scribes both phys­i­cal model and com­pu­ta­tion­al code

Campus & alumni news

Boston University Medicine was published by the Boston University Medical Campus, and presented stories on events and topics of interest to members of the BU Medical Campus community. It followed the discontinued publication Centerscope as Boston University Medicine from 1991-2005, then continued as Campus and Alumni News from 2006-2013 before returning to the title Boston University Medicine from 2014-present

Two-colour free electron laser with wide frequency separation using a single monoenergetic electron beam

Studies of a broad bandwidth, two-colour FEL amplier using one monoenergetic electron beam are presented. The two-colour FEL interaction is achieved using a series of undulator modules alternately tuned to two well-separated resonant frequencies. Using the broad bandwidth FEL simulation code Puffin, the electron beam is shown to bunch strongly and simultaneously at the two resonant frequencies. Electron bunching components are also generated at the sum and difference of the resonant frequencies

The implementation of 3D undulator fields in the unaveraged FEL simulation code Puffin

The FEL simulation code Puffin is modified to include 3D magnetic undulator fields. Puffin, having previously used a 1D undulator field, is modified to accommodate general 3D magnetic fields. Both plane and curved pole undulators have been implemented. The electron motion for both agrees with analytic predictions

Free-electron lasers : echoes of photons past

High-harmonic generation is an established method to significantly upshift laser photon energies. Now, researchers at the SLAC National Accelerator Laboratory have used echo concepts to generate coherent high-harmonic output from an electron-beam light source

Metabolomics of the bio-degradation process of aflatoxin B1 by actinomycetes at an initial pH of 6.0

Contamination of food and feed by Aflatoxin B1 (AFB1) is a cause of serious economic and health problems. Different processes have been used to degrade AFB1. In this study, biological degradation of AFB1 was carried out using three Actinomycete species, Rhodococcus erythropolis ATCC 4277, Streptomyces lividans TK 24, and S. aureofaciens ATCC 10762, in liquid cultures. Biodegradation of AFB1 was optimised under a range of temperatures from 25 to 40 °C and pH values of 4.0 to 8.0. An initial concentration of 20 µg/mL of AFB1 was used in this study. The amount of AFB1 remaining was measured against time by thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC), coupled with UV and mass spectrometry (LC-MS). All species were able to degrade the AFB1, and no significant difference was found between them. AFB1 remained in the liquid culture for R. erythropolis, S. lividans and S. aureofaciens were 0.81 µg/mL, 2.41 µg/mL and 2.78 µg/mL respectively, at the end of the first 24 h. Degradation occurred at all incubation temperatures and the pH with the optimal conditions for R. erythropolis was achieved at 30 °C and pH 6, whereas for S. lividans and S. aureofaciens the optimum conditions for degradation were 30 °C and pH 5. Analysis of the degradative route indicated that each microorganism has a different way of degrading AFB1. The metabolites produced by R. erythropolis were significantly different from the other two microorganisms. Products of degradation were identified through metabolomic studies by utilizing high-resolution mass spectral data. Mass spectrometric analysis indicated that the degradation of AFB1 was associated with the appearance of a range of lower molecular weight compounds. The pathway of degradation or chemical alteration of AFB1 was followed by means of high resolution Fourier transform mass spectrometry (HR-FTMS) analysis as well as through the MS2 fragmentation to unravel the degradative pathway for AFB1. AFB1 bio-degradation was coupled with the accumulation of intermediates of fatty acid metabolism and glycolysis. A plausible mechanism of degradation of AFB1 by Rhodococcus was hypothesized