HSP70 inhibits TNF-α induced nuclear translocation of NF-κB in FLSs, as detected using immunocytochemistry

Rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs) were incubated with heat shock protein (HSP)70 (1 μg/ml) for 1 hour, and the FLSs were washed and exposed to tumour necrosis factor (TNF)-α (20 ng/ml) for 30 minutes. The cells were fixed, permeabilized and incubated with rabbit anti-p65 antibody, followed by Cy3-conjugated anti-rabbit immunoglobulin (red). The nuclei of the corresponding cells were demonstrated by Hoechst 33258 staining. Total magnification for images was 200×.<p><b>Copyright information:</b></p><p>Taken from "Extracellular heat shock protein 70 inhibits tumour necrosis factor-α induced proinflammatory mediator production in fibroblast-like synoviocytes"</p><p>http://arthritis-research.com/content/10/2/R41</p><p>Arthritis Research & Therapy 2008;10(2):R41-R41.</p><p>Published online 14 Apr 2008</p><p>PMCID:PMC2453760.</p><p></p

HSP70 inhibits TNF-α induced IL-6, IL-8 and MCP-1 secretion in RA FLSs

Rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs) were incubated with the indicated concentrations of heat shock protein (HSP)70 or control protein OVA (10 μg/ml) for 1 hour, washed and then exposed to tumour necrosis factor (TNF)-α (20 ng/ml). The supernatants were harvested after 24 hours, and IL-6, IL-8 and monocyte chemoattractant protein (MCP)-1 concentrations were determined using ELISA. Data are presented as means ± standard deviation of three independent experiments. *< 0.05 versus the TNF-α stimulated group.<p><b>Copyright information:</b></p><p>Taken from "Extracellular heat shock protein 70 inhibits tumour necrosis factor-α induced proinflammatory mediator production in fibroblast-like synoviocytes"</p><p>http://arthritis-research.com/content/10/2/R41</p><p>Arthritis Research & Therapy 2008;10(2):R41-R41.</p><p>Published online 14 Apr 2008</p><p>PMCID:PMC2453760.</p><p></p