Genetic requirement for Ras in the transformation of fibroblasts and hematopoietic cells by the Bcr-Abl oncogene.

To determine the functional importance of Ras in transformation by Abl oncogenes, we used a genetic approach to measure the effect of impaired Ras activity on the ability of Bcr-Abl or v-Abl to transform cells. Expression of the catalytic domain of the GTPase activating protein for Ras (Gap C terminus) impaired soft agar colony formation by fibroblasts expressing v-Abl or Bcr-Abl by 70-80%. To test Ras function in a model that more closely resembles clinical diseases involving Bcr-Abl, double gene retroviruses expressing Bcr-Abl paired with the Gap C terminus or dominant negative Ras were introduced into naive mouse bone marrow cells. Transformation by Bcr-Abl was completely blocked in both situations. Coexpression of normal c-H-Ras accelerated the transforming activity of Bcr-Abl. These findings show that Ras activation is essential for the leukemogenic activity of Abl oncogenes in two distinct model systems. The results genetically define a connection between the Bcr-Abl cytoplasmic tyrosine kinase and Ras and add to the accumulating evidence that deregulation of Ras is a central event in the genesis of a number of molecularly distinct forms of human myeloid leukemia

A bright millisecond radio burst of extragalactic origin

Pulsar surveys offer one of the few opportunities to monitor even a small fraction (~0.00001) of the radio sky for impulsive burst-like events with millisecond durations. In analysis of archival survey data, we have discovered a 30-Jy dispersed burst of duration <5 ms located three degrees from the Small Magellanic Cloud. The burst properties argue against a physical association with our Galaxy or the Small Magellanic Cloud. Current models for the free electron content in the Universe imply a distance to the burst of <1 Gpc No further bursts are seen in 90-hr of additional observations, implying that it was a singular event such as a supernova or coalescence of relativistic objects. Hundreds of similar events could occur every day and act as insightful cosmological probes.Comment: 18 pages, 4 figures. Accepted by Science. Published electronically via Science Express on September 27, 200

Absence of Colossal Magnetoresistance in the Oxypnictide PrMnAsO0.95F0.05

ACKNOWLEDGMENTS This research is supported by the EPSRC (Research Grant EP/ L002493/1). We also acknowledge STFC-GB for provision of beamtime at ILL and ESRF.Peer reviewedPublisher PD