267 research outputs found

    Cultivating Institutional Capacities for Learning Analytics

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    Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/138371/1/he20243.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/138371/2/he20243_am.pd

    Surficial Geology of the Upper Iowa River Watershed: Phase 4: Cresco Ne 7.5\u27 Quadrangle,

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    https://ir.uiowa.edu/igs_ofm/1055/thumbnail.jp

    Bedrock Geology of the Upper Iowa River Watershed: Phase 4: Ridgeway 7.5\u27 Quadrangle,

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    https://ir.uiowa.edu/igs_ofm/1056/thumbnail.jp

    Bedrock Geology of the Davenport West 7.5\u27 Quadrangle, Scott County, Iowa

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    https://ir.uiowa.edu/igs_ofm/1064/thumbnail.jp

    Surficial Geology of the Upper Iowa River Watershed Phase 4: Ridgeway 7.5\u27 Quadrangle,

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    https://ir.uiowa.edu/igs_ofm/1057/thumbnail.jp

    Assessment of DNA extracted from FTA® cards for use on the Illumina iSelect BeadChip

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    <p>Abstract</p> <p>Background</p> <p>As FTA<sup>® </sup>cards provide an ideal medium for the field collection of DNA we sought to assess the quality of genomic DNA extracted from this source for use on the Illumina BovineSNP50 iSelect BeadChip which requires unbound, relatively intact (fragment sizes ≥ 2 kb), and high-quality DNA. Bovine blood and nasal swab samples collected on FTA cards were extracted using the commercially available GenSolve kit with a minor modification. The call rate and concordance of genotypes from each sample were compared to those obtained from whole blood samples extracted by standard PCI extraction.</p> <p>Findings</p> <p>An ANOVA analysis indicated no significant difference (P > 0.72) in BovineSNP50 genotype call rate between DNA extracted from FTA cards by the GenSolve kit or extracted from whole blood by PCI. Two sample t-tests demonstrated that the DNA extracted from the FTA cards produced genotype call and concordance rates that were not different to those produced by assaying DNA samples extracted by PCI from whole blood.</p> <p>Conclusion</p> <p>We conclude that DNA extracted from FTA cards by the GenSolve kit is of sufficiently high quality to produce results comparable to those obtained from DNA extracted from whole blood when assayed by the Illumina iSelect technology. Additionally, we validate the use of nasal swabs as an alternative to venous blood or buccal samples from animal subjects for reliably producing high quality genotypes on this platform.</p

    Surficial Geology of the Manly 7.5\u27\u27 Quadrangle, Worth and Cerro Gordo Counties, Iowa

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    https://ir.uiowa.edu/igs_ofm/1073/thumbnail.jp

    Surficial Geology of the Fertile SE 7.5\u27\u27 Quadrangle, Worth and Cerro Gordo Counties, Iowa

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    https://ir.uiowa.edu/igs_ofm/1075/thumbnail.jp

    Surficial Geology of the Northwood 7.5\u27 Quadrangle,

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    https://ir.uiowa.edu/igs_ofm/1083/thumbnail.jp
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