Prehension and perception of size in left visual neglect

Right hemisphere damaged patients with and without left visual neglect, and age-matched controls had objects of various sizes presented within left or right body hemispace. Subjects were asked to estimate the objects’ sizes or to reach out and grasp them, in order to assess visual size processing in perceptual-experiential and action-based contexts respectively. No impairments of size processing were detected in the prehension performance of the neglect patients but a generalised slowing of movement was observed, associated with an extended deceleration phase. Additionally both patient groups reached maximum grip aperture relatively later in the movement than did controls. For the estimation task it was predicted that the left visual neglect group would systematically underestimate the sizes of objects presented within left hemispace but no such abnormalities were observed. Possible reasons for this unexpected null finding are discussed

The U-band Galaxy Luminosity Function of Nearby Clusters

Despite the great potential of the U-band galaxy luminosity function (GLF) to constrain the history of star formation in clusters, to clarify the question of variations of the GLF across filter bands, to provide a baseline for comparisons to high-redshift studies of the cluster GLF, and to estimate the contribution of bound systems of galaxies to the extragalactic near-UV background, determinations have so far been hampered by the generally low efficiency of detectors in the U-band and by the difficulty of constructing both deep and wide surveys. In this paper, we present U-band GLFs of three nearby, rich clusters to a limit of M_U=-17.5 (M*_U+2). Our analysis is based on a combination of separate spectroscopic and R-band and U-band photometric surveys. For this purpose, we have developed a new maximum-likelihood algorithm for calculating the luminosity function that is particularly useful for reconstructing the galaxy distribution function in multi-dimensional spaces (e.g., the number of galaxies as a simultaneous function of luminosity in different filter bands, surface brightness, star formation rate, morphology, etc.), because it requires no prior assumptions as to the shape of the distribution function. The composite luminosity function can be described by a Schechter function with characteristic magnitude M*_U=-19.82+/-0.27 and faint end slope alpha_U=-1.09+/-0.18. The total U-band GLF is slightly steeper than the R-band GLF, indicating that cluster galaxies are bluer at fainter magnitudes. Quiescent galaxies dominate the cumulative U-band flux for M_U<-14. The contribution of galaxies in nearby clusters to the U-band extragalactic background is <1% Gyr^-1 for clusters of masses ~3*10^14 to 2*10^15 M_solar.Comment: 44 pages, 11 figures, accepted for publication in Ap

Quorum sensing influences growth and photosynthetic membrane production in high-cell-density cultivations of Rhodospirillum rubrum

BACKGROUND: The facultative anoxygenic photosynthetic bacterium Rhodospirillum rubrum exhibits versatile metabolic activity allowing the adaptation to rapidly changing growth conditions in its natural habitat, the microaerobic and anoxic zones of stagnant waters. The microaerobic growth mode is of special interest as it allows the high-level expression of photosynthetic membranes when grown on succinate and fructose in the dark, which could significantly simplify the industrial production of compounds associated with PM formation. However, recently we showed that PM synthesis is no longer inducible when R. rubrum cultures are grown to high cell densities under aerobic conditions. In addition a reduction of the growth rate and the continued accumulation of precursor molecules for bacteriochlorophyll synthesis were observed under high cell densities conditions. RESULTS: In the present work, we demonstrate that the cell density-dependent effects are reversible if the culture supernatant is replaced by fresh medium. We identified six N-acylhomoserine lactones and show that four of them are produced in varying amounts according to the growth phase and the applied growth conditions. Further, we demonstrate that N-acylhomoserine lactones and tetrapyrrole compounds released into the growth medium affect the growth rate and PM expression in high cell density cultures. CONCLUSIONS: In summary, we provide evidence that R. rubrum possesses a Lux-type quorum sensing system which influences the biosynthesis of PM and the growth rate and is thus likely to be involved in the phenotypes of high cell density cultures and the rapid adaptation to changing environmental conditions

The Galaxy Angular Correlation Functions and Power Spectrum from the Two Micron All Sky Survey

We calculate the angular correlation function of galaxies in the Two Micron All Sky Survey. We minimize the possible contamination by stars, dust, seeing and sky brightness by studying their cross correlation with galaxy density, and limiting the galaxy sample accordingly. We measure the correlation function at scales between 1-18 arcdegs using a half million galaxies. We find a best fit power law to the correlation function has a slope of 0.76 and an amplitude of 0.11. However, there are statistically significant oscillations around this power law. The largest oscillation occurs at about 0.8 degrees, corresponding to 2.8 h^{-1} Mpc at the median redshift of our survey, as expected in halo occupation distribution descriptions of galaxy clustering. We invert the angular correlation function using Singular Value Decomposition to measure the three-dimensional power spectrum and find that it too is in good agreement with previous measurements. A dip seen in the power spectrum at small wavenumber k is statistically consistent with CDM-type power spectra. A fit of CDM-type power spectra to k < 0.2 h Mpc^{-1} give constraints of \Gamma_{eff}=0.116 and \sigma_8=0.96. This suggest a K_s-band linear bias of 1.1+/-0.2. This \Gamma_{eff} is different from the WMAP CMB derived value. On small scales the power-law shape of our power spectrum is shallower than that derived for the SDSS. These facts together imply a biasing of these different galaxies that might be nonlinear, that might be either waveband or luminosity dependent, and that might have a nonlocal origin.Comment: 14 pages, 20 figures, to be published in ApJ January 20th, revision included two new figures, version with high resolution figures can be found here http::ww

Expanding P450 catalytic reaction space through evolution and engineering

Advances in protein and metabolic engineering have led to wider use of enzymes to synthesize important molecules. However, many desirable transformations are not catalyzed by any known enzyme, driving interest in understanding how new enzymes can be created. The cytochrome P450 enzyme family, whose members participate in xenobiotic metabolism and natural products biosynthesis, catalyzes an impressive range of difficult chemical reactions that continues to grow as new enzymes are characterized. Recent work has revealed that P450-derived enzymes can also catalyze useful reactions previously accessible only to synthetic chemistry. The evolution and engineering of these enzymes provides an excellent case study for how to genetically encode new chemistry and expand biology's reaction space

Demonstration of the protective effects of fluorescent proteins in baculoviruses exposed to ultraviolet light inactivation

Autographa californica multiple nucleopolyhedrovirus (AcMNPV) recombinants, namely AcRFP produced by fusion of the red fluorescent protein (RFP) gene with the polyhedrin gene, and a recombinant (pAcUW21-23GFP) carrying the green fluorescent protein (GFP) in its viral envelope, were evaluated for their resistance to inactivation by ultraviolet light. AcRFP recombinants produced incomplete polyhedra with low infectivity for Trichoplusia ni larvae, whereas AcuW21-23GFP produced normal polyhedra with high infectivity. Electron microscopy of AcRFP CL14 showed the incorporation of very few viral particles into polyhedrin matrix protein material. The LC(50) for AcuW21-23GFP was 0.10 occlusion bodies/mm(2), whereas the LC(50) values for several AcRFP recombinants ranged from 20 to 329 occlusion bodies/mm(2). When both the RFP and GFP recombinants were exposed to ultraviolet light (UV-B 280–320 nm), the results support the conclusion that these fluorescent proteins afford some protection against its damaging effects. Abbreviation: / AcMNPV: Autographa californica multiple nucleopolyhedrovirus BV: budded virus CPE: cytopathogenic effect ECV: extracellular virus OB: occlusion body ODV: occlusion derived virus RFP: red fluorescent protein GFP: green fluorescent protein TCID(50): tissue culture infective dose at the 50 % level UV-B: ultraviolet light of 280–320 n

Novel technique to extract experimental symmetry free energy information of nuclear matter

A new method of accessing information on the symmetry free energy from yields of fragments produced in Fermi-energy heavy-ion collisions is proposed. Furthermore, by means of quantum fluctuation analysis techniques, correlations between extracted symmetry free-energy coefficients with temperature and density were studied. The obtained results are consistent with those of commonly used isoscaling techniques.Comment: 6 pages, 3 figures Heavy-ion nuclear reactions at Fermi energies, Nuclear equation of State, Fragmentatio