RESCALE: Review and Simulate Climate and Catchment Responses at Burrishoole : Marine Research Sub-Programme (NDP 2007-’13) Series. ISSN 2009-3195

Abstract included in text

RESCALE: Review and Simulate Climate and Catchment Responses at Burrishoole - Project-Based Award, Final Summary Report

Sea Change—A Marine Knowledge, Research & Innovation Strategy for Ireland 2007-2013—was launched in early 2007 and was the outcome of extensive analysis and consultation with government departments, state agencies, industry and the third-level sector. It outlines a vision for the development of Ireland’s marine sector and sets clear objectives aimed at achieving this vision, namely to: 1. Assist existing, and largely indigenous, marine sub-sectors to improve their overall competitiveness and engage in activity that adds value to their outputs by utilising knowledge and technology arising from research. 2. Build new research capacity and capability and utilise fundamental knowledge and technology to create new marine-related commercial opportunities and companies. 3. Inform public policy, governance and regulation by applying the knowledge derived from marine research and monitoring. 4. Increase the marine sector’s competitiveness and stimulate the commercialisation of the marine resource in a manner that ensures its sustainability and protects marine biodiversity and ecosystems. 5. Strengthen the economic, social and cultural base of marine dependant regional/rural communities. The Sea Change strategy was developed as an integral part of the government’s Strategy for Science, Technology and Innovation (SSTI) and the Marine Institute as the lead implementation agency is working within SSTI policy and with government departments and agencies to deliver on the Strategy. The Marine Institute managed Marine Research Sub-Programme, one of eight sub-programmes within the Science, Technology and Innovation (STI) Programme of the National Development Plan 2007—2013, targets funding to meet the objectives of the Sea Change strateg

RESCALE: Review and Simulate Climate and Catchment Responses at Burrishoole - Project-Based Award, Final Summary Report

Sea Change—A Marine Knowledge, Research & Innovation Strategy for Ireland 2007-2013—was launched in early 2007 and was the outcome of extensive analysis and consultation with government departments, state agencies, industry and the third-level sector. It outlines a vision for the development of Ireland’s marine sector and sets clear objectives aimed at achieving this vision, namely to: 1. Assist existing, and largely indigenous, marine sub-sectors to improve their overall competitiveness and engage in activity that adds value to their outputs by utilising knowledge and technology arising from research. 2. Build new research capacity and capability and utilise fundamental knowledge and technology to create new marine-related commercial opportunities and companies. 3. Inform public policy, governance and regulation by applying the knowledge derived from marine research and monitoring. 4. Increase the marine sector’s competitiveness and stimulate the commercialisation of the marine resource in a manner that ensures its sustainability and protects marine biodiversity and ecosystems. 5. Strengthen the economic, social and cultural base of marine dependant regional/rural communities. The Sea Change strategy was developed as an integral part of the government’s Strategy for Science, Technology and Innovation (SSTI) and the Marine Institute as the lead implementation agency is working within SSTI policy and with government departments and agencies to deliver on the Strategy. The Marine Institute managed Marine Research Sub-Programme, one of eight sub-programmes within the Science, Technology and Innovation (STI) Programme of the National Development Plan 2007—2013, targets funding to meet the objectives of the Sea Change strateg

RESCALE: Review and Simulate Climate and Catchment Responses at Burrishoole : Marine Research Sub-Programme (NDP 2007-’13) Series. ISSN 2009-3195

Abstract included in text

RESCALE: Review and Simulate Climate and Catchment Responses at Burrishoole : Marine Research Sub-Programme (NDP 2007-’13) Series. ISSN 2009-3195

Abstract included in text

RESCALE: Review and Simulate Climate and Catchment Responses at Burrishoole : Marine Research Sub-Programme (NDP 2007-’13) Series. ISSN 2009-3195

Abstract included in text

RHEX, a novel regulator of human erythroid progenitor cell expansion and erythroblast development.

Ligation of erythropoietin (EPO) receptor (EPOR) JAK2 kinase complexes propagates signals within erythroid progenitor cells (EPCs) that are essential for red blood cell production. To reveal hypothesized novel EPOR/JAK2 targets, a phosphotyrosine (PY) phosphoproteomics approach was applied. Beyond known signal transduction factors, 32 new targets of EPO-modulated tyrosine phosphorylation were defined. Molecular adaptors comprised one major set including growth factor receptor-bound protein 2 (GRB2)-associated binding proteins 1-3 (GAB1-3), insulin receptor substrate 2 (IRS2), docking protein 1 (DOK1), Src homology 2 domain containing transforming protein 1 (SHC1), and sprouty homologue 1 (SPRY1) as validating targets, and SPRY2, SH2 domain containing 2A (SH2D2A), and signal transducing adaptor molecule 2 (STAM2) as novel candidate adaptors together with an ORF factor designated as regulator of human erythroid cell expansion (RHEX). RHEX is well conserved in Homo sapiens and primates but absent from mouse, rat, and lower vertebrate genomes. Among tissues and lineages, RHEX was elevated in EPCs, occurred as a plasma membrane protein, was rapidly PY-phosphorylated \u3e20-fold upon EPO exposure, and coimmunoprecipitated with the EPOR. In UT7epo cells, knockdown of RHEX inhibited EPO-dependent growth. This was associated with extracellular signal-regulated kinase 1,2 (ERK1,2) modulation, and RHEX coupling to GRB2. In primary human EPCs, shRNA knockdown studies confirmed RHEX regulation of erythroid progenitor expansion and further revealed roles in promoting the formation of hemoglobinizing erythroblasts. RHEX therefore comprises a new EPO/EPOR target and regulator of human erythroid cell expansion that additionally acts to support late-stage erythroblast development