19 research outputs found
BAG3 (Bcl-2-Associated Athanogene-3) Coding Variant in Mice Determines Susceptibility to Ischemic Limb Muscle Myopathy by Directing Autophagy
Understanding Elite Commitment to Social Protection: Rwanda's Vision 2020 Umurenge Programme
Modeling ecohydrological dynamics of smallholder strategies for food production in dryland agricultural systems
In dryland environments, characterized by low and frequently variable rainfall, smallholder farmers must take crop water sensitivity into account along with other characteristics like seed availability and market price when deciding what to plant. In this paper we use the results of surveys conducted among smallholders located near Mount Kenya to identify clusters of farmers devoting different fractions of their land to subsistence and market crops. Additionally, we explore the tradeoffs between water-insensitive but low-value subsistence crops and a water-sensitive but high-value market crop using a numerical model that simulates soil moisture dynamics and crop production over multiple growing seasons. The cluster analysis shows that most farmers prefer to plant either only subsistence crops or only market crops, with a minority choosing to plant substantial fractions of both. The model output suggests that the value a farmer places on a successful growing season, a measure of risk aversion, plays a large role in whether the farmer chooses a subsistence or market crop strategy. Furthermore, access to irrigation, makes market crops more appealing, even to very risk-averse farmers. We then conclude that the observed clustering may result from different levels of risk aversion and access to irrigation
BAG3 (Bcl-2-Associated Athanogene-3) Coding Variant in Mice Determines Susceptibility to Ischemic Limb Muscle Myopathy by Directing Autophagy
Muscle cell derived angiopoietin-1 contributes to both myogenesis and angiogenesis in the ischemic environment
Recent strategies to treat peripheral arterial disease (PAD) have focused on stem cell based therapies, which are believed to result in local secretion of vascular growth factors. Little is known, however, about the role of ischemic endogenous cells in this context. We hypothesized that ischemic muscle cells (MC) are capable of secreting growth factors that act as potent effectors of the local cellular regenerative environment. Both muscle and endothelial cells (ECs) were subjected to experimental ischemia, and conditioned medium (CM) from each was collected and analyzed to assess myogenic and/or angiogenic potential. In muscle progenitors, mRNA expression of VEGF and its cognate receptors (Nrp1, Flt, Flk) was present and decreased during myotube formation in vitro, and EC CM or VEGF increased myoblast proliferation. Angiopoietin-1 (Ang-1), Tie1, and Tie2 mRNA increased during MC differentiation in vitro. Exogenous Ang-1 enhanced myogenic (MyoD and Myogenin) mRNA in differentiating myoblasts and increased myosin heavy chain protein. Myotube formation was enhanced by MC CM and inhibited by EC CM. Ang-1 protein was present in CM from MCs isolated from both the genetically ischemia-susceptible BALB/c and ischemia-resistant C57BL/6 mouse strains, and chimeric Tie2 receptor trapping in situ ablated Ang-1's myogenic effects in vitro. Ang-1 or MC CM enhanced myotube formation in a mixed isolate of muscle progenitors as well as a myoblast co-culture with pluripotent mesenchymal cells (10T1/2) and this effect was abrogated by viral expression of the extracellular domain of Tie2 (AdsTie2). Furthermore, mesh/tube formation by HUVECs was enhanced by Ang-1 or MC CM and abrogated by Tie2 chimeric receptor trapping. Our results demonstrate the ability of muscle and endothelial cell-derived vascular growth factors, particularly Ang-1, to serve as multi-functional stimuli regulating crosstalk between blood vessels and muscle cells during regeneration from ischemic myopathy
Vestaâs mineralogical composition as as revealed by the visible and infrared spectrometer on Dawn
The Dawn spacecraft mission has provided extensive new and detailed data on Vesta that confirm and strengthen the Vestaâhowarditeâeucriteâdiogenite (HED) meteorite link and the concept that Vesta is differentiated, as derived from earlier telescopic observations. Here, we present results derived by newly calibrated spectra of Vesta. The comparison between data from the Dawn imaging spectrometerâVIRâand the different class of HED meteorites shows that average spectrum of Vesta resembles howardite spectra. Nevertheless, the Vesta spectra at high spatial resolution reveal variations in the distribution of HED-like mineralogies on the asteroid. The data have been used to derive HED distribution on Vesta, reported in Ammannito et al. (2013), and to compute the average Vestan spectra of the different HED lithologies, reported here. The spectra indicate that, not only are all the different HED lithologies present on Vesta, but also carbonaceous chondritic material, which constitutes the most abundant inclusion type found in howardites, is widespread. However, the hydration feature used to identify carbonaceous chondrite material varies significantly on Vesta, revealing different band shapes. The characteristic of these hydration features cannot be explained solely by infalling of carbonaceous chondrite meteorites and other possible origins must be considered. The relative proportion of HEDs on Vesta's surface is computed, and results show that most of the vestan surface is compatible with eucrite-rich howardites and/or cumulate or polymict eucrites. A very small percentage of surface is covered by diogenite, and basaltic eucrite terrains are relatively few compared with the abundance of basaltic eucrites in the HED suite. The largest abundance of diogenitic material is found in the Rheasilvia region, a deep basin, where it clearly occurs below a basaltic upper crust. However, diogenite is also found elsewhere; although the depth to diogenite is consistent with one magma ocean model, its lateral extent is not well constrained