Variation in the circularly polarized light reflection of Lomaptera (Scarabaeidae) beetles

An extended spectroscopic study on the left-through-left circularly polarised reflection spectra of a large number of beetles from the Australasian Scrabaeidae:Cetoniinae of the Lomaptera genus was undertaken. We have obtained a five-category spectral classification.The principal spectral features, which even within the genus range from blue to infra-red, are related to structural chirality in the beetle shells. The detailed features of each spectral classification are related to different structural perturbations of the helix including various pitch values and abrupt twist defects. These spectral characteristics and associated shell structures are confirmed on the basis of simple modelling. An important conclusion from our study is that the simple helical structure resulting in a single symmetric Bragg peak is not the dominant spectral type. Rather the reality is a rich tapestry of spectral types. One intriguing specimen is identified via an SEM micrograph to consist of a double interstitial helix leadingto a particular double-peak spectrum

Cultural Predilection On Design In Planning Ecological Sanitation In Northern Uganda

Poor sanitation and open defecation is a major concern in many parts of the world. Ecological sanitation is a possible alternative to the global issue by using dry toilet systems that allow for recycling the waste for other uses. The purpose of this thesis is to determine the cultural perception and design implications toward ecological sanitation in developing countries. By surveying the local population in the proposed environments and implementing a corresponding system, the sanitation system has a much higher chance of sustainability and success. In Northern Uganda, the population was surveyed through a questionnaire and a Visual Preference Survey to determine current conditions, immediate needs, cultural preferences, and attitudes toward the design of sanitation types with the main focus on privacy, placement, type and gender-specific facilities. Results demonstrated that the majority of the population was interested in learning more to improve sanitation and reuse waste for agricultural productivity

Trends in potential exposure to Australian bat lyssavirus in South East Queensland, 1996 to 2003

This study examined trends in notifications of potential exposure to Australian bat lyssavirus reported to the Brisbane Southside Public Health Unit, Australia between 1 November 1996 and 31 January 2003. Notification rates declined among all population groups and potential exposures were notified more promptly. Concern exists regarding possible under-reporting of potential exposure to Australian bat lyssavirus especially among volunteer bat carers. (author abstract

Model-Based Diagnosis in a Power Distribution Test-Bed

The Rodon model-based diagnosis shell was applied to a breadboard test-bed, modeling an automated power distribution system. The constraint-based modeling paradigm and diagnostic algorithm were found to adequately represent the selected set of test scenarios

Investigation of the variation of near-circular polarization in Scarabaeoidea beetles

Variation in the reflection of circularly polarized light (CP) of a substantial number of beetles, of both the Hybosoridae and Scarabaeidae families, is discussed. Classifications of the spectral shapes were made for Cetonia aurata aurata beetles, which were related to variations within the chiral chitin structure and have been computationally modelled. It was seen that single peaked spectra were not the predominant spectral shape and that more complex structures are responsible for the spectra observed. Two structural perturbations methods to the single pitched structure are proposed to be responsible for the more complex spectral shapes. Further CP analysis of the genus rutelinae:Chrysina was undertaken with variations in broadband reflection observed within the optima species

Phosphorylation of androgen receptors at serine 515 is a potential prognostic marker for triple negative breast cancer

1.7 million cases of breast cancer are diagnosed every year with 522,000 deaths. Molecular classifications of breast cancer have resulted in improved treatments. However, treatments for triple negative breast cancer (TNBC) are lacking. Analysis of molecular targets for TNBC is a priority. One potential candidate is androgen receptor (AR) phosphorylation. This study assessed the role of AR phosphorylation at ser81/ser515 and their two upstream effectors, cyclin-dependent kinase 1 (pCDK1) and extracellular-regulated kinase 1/2 (pERK1/2) in 332 ductal breast cancer patients by immunohistochemistry. pERK1/2 combined with AR-515 associated with improved cancer-specific survival (CSS, p = 0.038), decreased size (p = 0.001), invasive grade (p < 0.001), necrosis (p = 0.003), b-lymphocytes (p = 0.020), molecular subtype (p < 0.001) and estrogen receptor (ER)/progesterone receptor (PR)-status (p < 0.001). The cohort was therefore stratified into ER+ve and ER-ve patients. In ER+ve tumours, pERK1/2 combined with AR-515 associated with improved CSS (p = 0.038), smaller size (p = 0.004), invasive grade (p = 0.001), decreased b-lymphocytes (p = 0.013) and increased plasma cells (p = 0.048). In contrast, in TNBC patients, phosphorylation of AR-515 associated with poorer CSS (p = 0.007). pERK1/2 combined with AR-515 associated with decreased inflammation (p = 0.003), increased tumour stroma (p = 0.003) and tumour budding (p = 0.011), with trends towards decrease CSS (p = 0.065) and macrophage levels (p = 0.093). In Conclusions, AR-515 may be an important regulator of inflammation in breast cancer potential via ERK1/2 phosphorylation. AR-515 is a potential prognostic marker and therapeutic target for TNBC

Loss of signal transducer and activator of transcription 1 is associated with prostate cancer recurrence

STAT1 loss has previously been implicated in cell line studies to modify prostate cancer cell growth and survival, however the clinical significance of this has not previously been established. This study investigated if STAT1 loss was associated with patient outcome measures and the phenotypic consequence of STAT1 silencing. STAT1 expression was assessed in two patient cohorts with localised (n = 78) and advanced prostate cancer at initial diagnosis (n = 39) by immunohistochemistry (IHC). Impact of STAT1 silencing on prostate cancer cells lines was assessed using Cell Death detection ELISA, TLDA gene signature apoptosis arrays, WST-1 assay, xCELLigence system, clonogenic assay, and wound healing assay. In the localised patient cohort, low expression of STAT1 was associated with shorter time to disease recurrence (3.8 vs 7.3 years, P = 0.02) and disease specific survival (6.6 vs 9.3 years, P = 0.05). In the advanced patient cohort, low expression was associated with shorter time to disease recurrence (2.0 vs 3.9 years, P = 0.001). When STAT1 was silenced in PC3 cells (AR negative) and LNCaP cells (AR positive) silencing did not influence levels of apoptosis in either cell line and had little effect on cell viability in the LNCaP cells. In contrast, STAT1 silencing in the PC3 cells resulted in a pronounced increase in cell viability (WST-1 assay: mock silenced vs STAT1 silenced, P < 0.001), clonagenicity (clonogenic assay: mock silenced vs STAT1 silenced, P < 0.001), and migration (wound healing: mock silenced vs STAT1 silenced, P < 0.001). In conclusion, loss of STAT1 may promote prostate cancer recurrence in AR negative patients via increasing cell viability