Use of cell morphology to evaluate the effect of a peroxidase gene on cell death induction thresholds in tobacco

Tobacco suspension cultures were subjected to a range of heat stresses and used to compare morphological aspects of programmed cell death (PCD) and necrosis. Cells undergoing PCD were found to display characteristic death morphology, caused by cytoplasmic retraction of the protoplast, and to have cleaved DNA. We evaluated if the morphological characteristics of PCD could be used to monitor changes in cell death induction thresholds in transgenic cell cultures with high levels of peroxidase activity. Again, using a heat shock assay, we show that tobacco cell cultures with elevated levels of peroxidase have higher cell death induction threshold levels than wild type tobacco cell cultures. Thus, assessing PCD associated morphological changes can report on the effect of altering peroxidase genes on cell death activation in tobacco. This study demonstrates that PCD morphology could routinely be used to monitor the effects of introduced genes on programmed cell death induction thresholds in plants

The Lantern Vol. 26, No. 2, Spring 1958

• The Wise Man • Of Men and Lobsters • The Painting • The Ghost of Moon Mountain • Song for the Atomic Age • Opus I • Stillnesshttps://digitalcommons.ursinus.edu/lantern/1074/thumbnail.jp

The Ursinus Weekly, March 10, 1958

Spray of Springtime theme of the junior prom, Mar. 21 • Group production of French farce now in rehearsal • Fireside chats and art forum on Y agenda for March • IFC weekend, Apr. 25-26; Dances, picnic planned • Roland\u27s rendezvous theme of soph hop, Sat. • Band members at festival • Council reviews rules; Revises judiciary powers • Fraternities bid men today at noon • Selective Service department test applications due • Ursinus alumni to hold a dinner dance, March 22 • Curtain Club to present famous art historian tomorrow, Mar. 11 • Final week of Campus Chest drive; Goal $1500 • 105 on Dean\u27s list for Fall semester • Gold in the hills adds to 1958 Ruby coffers • Editorial: Give once for all • Letters to the editor • Review: Gold in the hills • Time has come • Off the cuff fashions for men • \u2758 basketball season roundup shows 1 win • Badminton belles win two games • Belles down Beaver 56-50 for third win in a row • U.C. dunks Drexel; Loses to Chestnut Hill, Bryn Mawr • Many fields included in Feb. Library accessions • Special feature: Hands • R. Usher in officer traininghttps://digitalcommons.ursinus.edu/weekly/1402/thumbnail.jp

The Ursinus Weekly, May 26, 1958

MSGA plans tentative 1958 customs program • Junior advisory committee named • Big-little sister committee asks for applicants • Many U.C. seniors accepted at post-grad schools • Last Y commission meetings are held to plan for next Fall semester • R.G. Dunlop to speak at June 9th graduation • Ursinus\u27 Rowe gets scholarship • Editorial: Customs • Letters to the editor • Are fraternities? • Cindermen whip Mules, Dutchmen; Take three meets • Netmen finish season; Defeat Elizabethtown, 6-3 • Bears win 10th game; Lose final tilt by 8-1 • Girls\u27 tennis team beats Penn; Finish undefeated • Beta Sigma Lambda beats Sigma Rho Lambda in 9th • New Library accessionshttps://digitalcommons.ursinus.edu/weekly/1409/thumbnail.jp

The Lantern Vol. 26, No. 3, June 1958

• Reflections • Recession: A Matter of Opinion • His Name Was... • The Outward Bound • A Champion There Was • Notes from the Sukura • Undauntedhttps://digitalcommons.ursinus.edu/lantern/1075/thumbnail.jp

Quantum Optics and Photonics

Contains table of contents for Part II, table of contents for Section 1, and reports on six research projects.Charles S. Draper Laboratories Contract DL-H-418468U.S. Air Force - Electronic Systems Division Contract F19628-89-K-0300U.S. Navy - Office of Naval Research Grant N0014-91-J-1808U.S. Air Force - Electronic Systems Division Contract F19628-89-K-003

The Spitzer c2d Survey of Large, Nearby, Interstellar Clouds. VII. Ophiuchus Observed with MIPS

We present maps of 14.4 deg^2 of the Ophiuchus dark clouds observed by the Spitzer Space Telescope Multiband Imaging Photometer for Spitzer (MIPS). These high-quality maps depict both numerous point sources and extended dust emission within the star-forming and non–star-forming portions of these clouds. Using PSF-fitting photometry, we detect 5779 sources at 24 μm and 81 sources at 70 μm at the 10 σ level of significance. Three hundred twenty-three candidate young stellar objects (YSOs) were identified according to their positions on the MIPS/2MASS K versus color-magnitude diagrams, as compared to 24 μm detections in the SWIRE extragalactic survey. We find that more than half of the YSO candidates, and almost all those with protostellar Class I spectral energy distributions, are confined to the known cluster and aggregates

Combined Vorinostat and Chloroquine Inhibit Sodium Iodide Symporter Endocytosis and Enhance Radionuclide Uptake In Vivo

Purpose Patients with aggressive thyroid cancer are frequently failed by the central therapy of ablative radioiodide (RAI) uptake, due to reduced plasma membrane (PM) localization of the sodium/iodide symporter (NIS). We aimed to understand how NIS is endocytosed away from the PM of human thyroid cancer cells, and whether this was druggable in vivo.Experimental DesignInformed by analysis of endocytic gene expression in patients with aggressive thyroid cancer, we used mutagenesis, NanoBiT interaction assays, cell surface biotinylation assays, RAI uptake and NanoBRET to understand the mechanisms of NIS endocytosis in transformed cell lines and patient-derived human primary thyroid cells. Systemic drug responses were monitored via 99mTc pertechnetate gamma counting and gene expression in BALB/c mice.ResultsWe identify an acidic dipeptide within the NIS C-terminus which mediates binding to the 2 subunit of the Adaptor Protein 2 (AP2) heterotetramer. We discovered that the FDA-approved drug chloroquine modulates NIS accumulation at the PM in a functional manner that is AP2 dependent. In vivo, chloroquine treatment of BALB/c mice significantly enhanced thyroidal uptake of 99mTc pertechnetate in combination with the histone deacetylase (HDAC) inhibitor vorinostat/ SAHA, accompanied by increased thyroidal NIS mRNA. Bioinformatic analyses validated the clinical relevance of AP2 genes with disease-free survival in RAI-treated DTC, enabling construction of an AP2 gene-related risk score classifier for predicting recurrence.ConclusionsNIS internalisation is specifically druggable in vivo. Our data therefore provide new translatable potential for improving RAI therapy using FDA-approved drugs in patients with aggressive thyroid cancer.<br/