Phytochemical analysis of leaves and stems of Physalis alkekengi L. (Solanaceae)

Physalis alkekengi L. (Solanaceae) is encountered in different regions of Bulgaria as a wild growing or ornamental plant. The objective of this work was to characterize the phytochemical composition (macro and micro components) of the leaves and stems of two local phenotypes (PA-SB and PA-NB), with the view of revealing their use potential. The dry leaves contained (DW) protein (16.25 and 19.27%), cellulose (25.16 and 25.31%), and ash (18.28 and 16.16%) and the stems contained protein (6.83 and 7.35%), cellulose (39.34 and 38.25%), and ash (15.01 and 7.48%) for PA-SB and PA-NB, respectively. The dominant amino acids (by HPLC) in the leaves of both phenotypes were arginine (21.3-22.3 mg/g) and aspartic acid (8.8-18.4 mg/g), and those in the stems were proline and aspartic acid for PA-SB (8.8, 7.7 mg/g); isoleucine and tyrosine for PA-NB (12.8, 6.6 mg/g). Mineral elements, determined by AAS (K, Ca, Mg, Na, Cu, Fe, Zn, Mn, Pb, Cr), also varied between phenotypes and plant parts. The leaves alone were further processed by extraction with n-hexane, for the identification of leaf volatiles (by gas chromatography-mass spectrometry). The analysis identified 28 components (97.99%) in the leaf extract of PA-SB and 32 components (97.50%) in that of PA-NB. The volatile profile of PA-SB leaves was dominated by diterpenes (49.96%) and oxygenated sesquiterpenes (35.61%), while that of PA-NB was dominated by oxygenated aliphatics (40.01%) and diterpenes (35.19%). To the best of our knowledge, the study provides the first data about the phytochemical composition of the leaves and stems of P. alkekengi from Bulgaria, in a direct comparison of phenotypes from two distinct wild populations, which could be of further scientific interest.AlMaarefa University, Riyadh, Saudi Arabia; [2021-29]The authors deeply acknowledge the Researchers Supporting Program (TUMA Project-2021-29), AlMaarefa University, Riyadh, Saudi Arabia, for supporting steps of this work

Resinoid from cape gooseberry fruit (Physalis peruviana L.) - volatile composition and application as an active ingredient in a cosmetic formulation

Cape gooseberry (Physalis peruviana L.), an exotic fruit gaining popularity in Bulgaria, has been recognized as a highly functional food, but has also the potential to be a resource for the fragrance and cosmetic industries. The main objective of this study was to assess the potential of the resinoid (a type of traditional aromatic products) obtained from locally-produced fruit (variety “Plovdiv”) for use in cosmetics, by revealing its volatile composition and characterizing the properties of an author-developed resinoid- enriched cosmetic cream. The resinoid (yield 58.78% DW) was a dark-orange viscous mass, with fruity, caramel notes and smoke accords odor. The GC-MS analysis identified 44 volatiles (98.69%), representing carbohydrates, alcohols, acids, along with minor miscellaneous compounds. A cosmetic cream (O/W emulsion) was developed, incorporating fruit resinoid (1.00%) as an active ingredient, compared to a control sample. Resinoid inclusion affected positively the sensory and physico-chemical properties of the cosmetic cream (color, odor, pH, stability). The studied emulsions had the rheological behavior of pseudoplastic non-Newtonian fluids, and resinoid presence reduced cream viscosity. It could be concluded that Cape gooseberry fruit resinoid had the potential to be a reasonable ingredient in cosmetic preparations; of course, further research is needed to assess its complex effects

Chemical composition assessment of structural parts (seeds, peel, pulp) of physalis alkekengi l. fruits

In recent years there has been an extensive search for nature-based products with functional potential. All structural parts of Physalis alkekengi (bladder cherry), including fruits, pulp, and less-explored parts, such as seeds and peel, can be considered sources of functional macro- and micronutrients, bioactive compounds, such as vitamins, minerals, polyphenols, and polyunsaturated fatty acids, and dietetic fiber. The chemical composition of all fruit structural parts (seeds, peel, and pulp) of two phenotypes of P. alkekengi were studied. The seeds were found to be a rich source of oil, yielding 14-17%, with abundant amounts of unsaturated fatty acids (over 88%) and tocopherols, or vitamin E (up to 5378 mg/kg dw; dry weight). The predominant fatty acid in the seed oils was linoleic acid, followed by oleic acid. The seeds contained most of the fruit's protein (16-19% dw) and fiber (6-8% dw). The peel oil differed significantly from the seed oil in fatty acid and tocopherol composition. Seed cakes, the waste after oil extraction, contained arginine and aspartic acid as the main amino acids; valine, phenylalanine, threonine, and isoleucine were present in slightly higher amounts than the other essential amino acids. They were also rich in key minerals, such as K, Mg, Fe, and Zn. From the peel and pulp fractions were extracted fruit concretes, aromatic products with specific fragrance profiles, of which volatile compositions (GC-MS) were identified. The major volatiles in peel and pulp concretes were beta-linalool, alpha-pinene, and gamma-terpinene. The results from the investigation substantiated the potential of all the studied fruit structures as new sources of bioactive compounds that could be used as prospective sources in human and animal nutrition, while the aroma-active compounds in the concretes supported the plant's potential in perfumery and cosmetics.IGA FT 2022/004Tomas Bata University in Zlin, Faculty of Technology [IGA FT 2022/004

Resinoid from cape gooseberry fruit

Cape gooseberry (Physalis peruviana L.), an exotic fruit gaining popularity in Bulgaria, has been recognized as a highly functional food, but has also the potential to be a resource for the fragrance and cosmetic industries. The main objective of this study was to assess the potential of the resinoid (a type of traditional aromatic products) obtained from locally-produced fruit (variety “Plovdiv”) for use in cosmetics, by revealing its volatile composition and characterizing the properties of an author-developed resinoid- enriched cosmetic cream. The resinoid (yield 58.78% DW) was a dark-orange viscous mass, with fruity, caramel notes and smoke accords odor. The GC-MS analysis identified 44 volatiles (98.69%), representing carbohydrates, alcohols, acids, along with minor miscellaneous compounds. A cosmetic cream (O/W emulsion) was developed, incorporating fruit resinoid (1.00%) as an active ingredient, compared to a control sample. Resinoid inclusion affected positively the sensory and physico-chemical properties of the cosmetic cream (color, odor, pH, stability). The studied emulsions had the rheological behavior of pseudoplastic non-Newtonian fluids, and resinoid presence reduced cream viscosity. It could be concluded that Cape gooseberry fruit resinoid had the potential to be a reasonable ingredient in cosmetic preparations; of course, further research is needed to assess its complex effects

Effect of maturity on the phytonutrient composition of Cape gooseberry seeds (

The seeds of Cape gooseberry (Physalis peruviana L.) remain as a by-product from juice production, but they can also be a potential resource of valuable nutrients. The objective of this study was to analyze the phytonutrient composition of the seeds isolated from fruit at two maturity stages unsuitable for regular realization (unripe and under-ripe), with the aim of identifying the possibility for utilization of such non-standard or discarded agricultural production. The results showed about 3-time lower oil content in the unripe seeds than in the under-ripe seeds (6.60% vs. 21.75%), but no significant differences in the content of protein (18.44% and 17.83%) and cellulose (26.63% and 26.14%). The total tocopherol content slightly decreased with the progress of fruit maturity (from 8354 mg/kg to 7118 mg/kg). Significant changes in the amino acid composition were found only with regard to aspartic acid, glutamic acid, tyrosine and methionine. The content of macro and micro minerals was lower in the under-ripe seeds (about 1.5-2-time). The results from this baseline study suggested that the seeds from both unripe and under-ripe fruit could also be considered for nutritive purposes (animal feed, food products)

Chemical Composition Assessment of Structural Parts (Seeds, Peel, Pulp) of Physalis alkekengi L. Fruits

In recent years there has been an extensive search for nature-based products with functional potential. All structural parts of Physalis alkekengi (bladder cherry), including fruits, pulp, and less-explored parts, such as seeds and peel, can be considered sources of functional macro- and micronutrients, bioactive compounds, such as vitamins, minerals, polyphenols, and polyunsaturated fatty acids, and dietetic fiber. The chemical composition of all fruit structural parts (seeds, peel, and pulp) of two phenotypes of P. alkekengi were studied. The seeds were found to be a rich source of oil, yielding 14–17%, with abundant amounts of unsaturated fatty acids (over 88%) and tocopherols, or vitamin E (up to 5378 mg/kg dw; dry weight). The predominant fatty acid in the seed oils was linoleic acid, followed by oleic acid. The seeds contained most of the fruit’s protein (16–19% dw) and fiber (6–8% dw). The peel oil differed significantly from the seed oil in fatty acid and tocopherol composition. Seed cakes, the waste after oil extraction, contained arginine and aspartic acid as the main amino acids; valine, phenylalanine, threonine, and isoleucine were present in slightly higher amounts than the other essential amino acids. They were also rich in key minerals, such as K, Mg, Fe, and Zn. From the peel and pulp fractions were extracted fruit concretes, aromatic products with specific fragrance profiles, of which volatile compositions (GC-MS) were identified. The major volatiles in peel and pulp concretes were β-linalool, α-pinene, and γ-terpinene. The results from the investigation substantiated the potential of all the studied fruit structures as new sources of bioactive compounds that could be used as prospective sources in human and animal nutrition, while the aroma-active compounds in the concretes supported the plant’s potential in perfumery and cosmetics

Phytonutrient Composition of Two Phenotypes of <i>Physalis alkekengi</i> L. Fruit

Physalis alkekengi L. is the only representative of the genus Physalis (Solanaceae) that is native to Bulgaria, found in wild habitats under different climatic and soil conditions. The plant is poisonous, but produces edible fruit, which are a source of functional nutrients—vitamins, phenolic antioxidants, minerals, etc. Therefore, the objective of this work was to determine the presence of certain nutrient and bioactive substances in two phenotypes of P. alkekengi fruit from Bulgaria, in order to better reveal the prospects of fruit use in nutrition. Different macro and micronutrients were determined in the fruit—protein, ash, lipids, fiber, natural pigments, sugars, amino acids, minerals—and the results showed differences between the phenotypes. Fruit energy values were low and identical in the samples, 43 kcal/100 g. The fruits were rich in extractable phenolics (TPC, 17.74–20.25 mg GAE/100 g FW; flavonoids, 15.84–18.03 mg QE/100 g FW) and demonstrated good antioxidant activity (DPPH, 171.55–221.26 mM TE/g; FRAP, 193.18–256.35 mM TE/g). P. alkekengi fruits were processed to obtain a dry extract with ethanol (yield 47.92–58.6%), and its individual composition was identified (GC-MS). The results in this study supported the presumed phytonutritive potential of P. alkekengi fruit, thus, opening doors for further research