Decay rate asymmetry for B→XsγB \to X_s \gamma in SUSY model

We discuss a rate asymmetry for the radiative $B$-meson decay $B \to X_s \gamma$ within the framework of the supersymmetric standard model based on N=1 supergravity. This model contains new sources of CP violation. In spite of severe experimental constraints on the electric dipole moment of the neutron, a new CP-violating phase may not be suppressed, leading to a sizable enhancement of the decay rate asymmetry. The magnitude of the asymmetry is predicted to be larger than that by the standard model in wide parameter regions where the branching ratio is consistent with its experimental bounds. A possible maximal magnitude is about 0.1, which will be well accessible at B factories.Comment: 4 pages, Talk presented at the UK Phenomenology Workshop on Heavy Flavour and CP Violation, 17 - 22, September 2000, St John's College, Durha

Determinan Pemberantasan Sarang Nyamuk Dengue pada Rumah Tangga di Bogor Tahun 2016

Determinants of dengue mosquito eradication (PSN) in the households in Bogor in 2016PurposeThis study aimed to determine the factors that affect of PSN on housewives in Cibinong and the factors found that influence were: job, knowledge, attitude, behavior of housewife, counselling, active jumantik, giving abatement and the number of container.MethodsA cross-sectional study was conducted using 125 housewifes in Cibinong sub-district. The statistical analysis used chi-square and multiple logistic regression tests.ResultsThis study showed that counseling and the existence of jumantik cadre were the determinants of the application of mosquito nest eradication. Counseling was the most influential factor on the application of mosquito nest eradication.Conclusion Dengue control and prevention counseling should be given to all age groups in the community regularly and it is recommended to reactivate jumantik cadres

Lepton Flavour Violating tau Decays in the Left-Right Symmetric Model

The Left-Right symmetric extension of the Standard Model with Higgs isospin triplets can provide neutrino masses via a TeV scale seesaw mechanism. The doubly charged Higgs bosons H^{\pm\pm}_L and H^{\pm\pm}_R induce lepton flavour violating decays \tau^\pm \to lll at tree-level via a coupling which is related to the Maki-Nakagawa-Sakata matrix (V_{\rm MNS}). We study the magnitude and correlation of \tau^\pm \to lll and \mu\to e\gamma with specific assumptions for the origin of the large mixing in V_{\rm MNS} while respecting the stringent bound for \mu\to eee. It is also shown that an angular asymmetry for \tau^\pm \to lll is sensitive to the relative strength of the H^{\pm\pm}_L and H^{\pm\pm}_R mediated contributions and provides a means of distinguishing models with doubly charged Higgs bosons.Comment: 24 pages, 16 figures, added references, notation clarified, added discussion of effect of Majorana phases, version to appear in Physical Review

Implementation of earlier antibiotic administration in patients with severe sepsis and septic shock in Japan: a descriptive analysis of a prospective observational study.

BACKGROUND: Time to antibiotic administration is a key element in sepsis care; however, it is difficult to implement sepsis care bundles. Additionally, sepsis is different from other emergent conditions including acute coronary syndrome, stroke, or trauma. We aimed to describe the association between time to antibiotic administration and outcomes in patients with severe sepsis and septic shock in Japan. METHODS: This prospective observational study enrolled 1184 adult patients diagnosed with severe sepsis based on the Sepsis-2 criteria and admitted to 59 intensive care units (ICUs) in Japan between January 1, 2016, and March 31, 2017, as the sepsis cohort of the Focused Outcomes Research in Emergency Care in Acute Respiratory Distress Syndrome, Sepsis and Trauma (FORECAST) study. We compared the characteristics and in-hospital mortality of patients administered with antibiotics at varying durations after sepsis recognition, i.e., 0-60, 61-120, 121-180, 181-240, 241-360, and 361-1440 min, and estimated the impact of antibiotic timing on risk-adjusted in-hospital mortality using the generalized estimating equation model (GEE) with an exchangeable, within-group correlation matrix, with "hospital" as the grouping variable. RESULTS: Data from 1124 patients in 54 hospitals were used for analyses. Of these, 30.5% and 73.9% received antibiotics within 1 h and 3 h, respectively. Overall, the median time to antibiotic administration was 102 min [interquartile range (IQR), 55-189]. Compared with patients diagnosed in the emergency department [90 min (IQR, 48-164 min)], time to antibiotic administration was shortest in patients diagnosed in ICUs [60 min (39-180 min)] and longest in patients transferred from wards [120 min (62-226)]. Overall crude mortality was 23.4%, where patients in the 0-60 min group had the highest mortality (28.0%) and a risk-adjusted mortality rate [28.7% (95% CI 23.3-34.1%)], whereas those in the 61-120 min group had the lowest mortality (20.2%) and risk-adjusted mortality rates [21.6% (95% CI 16.5-26.6%)]. Differences in mortality were noted only between the 0-60 min and 61-120 min groups. CONCLUSIONS: We could not find any association between earlier antibiotic administration and reduction in in-hospital mortality in patients with severe sepsis

Administration route-dependent vaccine efficiency of murine dendritic cells pulsed with antigens

 Dendritic cells (DCs) loaded with tumour antigens have been successfully used to induce protective tumour immunity in murine models and human trials. However, it is still unclear which DC administration route elicits a superior therapeutic effect. Herein, we investigated the vaccine efficiency of DC2.4 cells, a murine dendritic cell line, pulsed with ovalbumin (OVA) in the murine E.G7-OVA tumour model after immunization via various routes. After a single vaccination using 1 × 106OVA-pulsed DC2.4 cells, tumour was completely rejected in the intradermally (i.d.; three of four mice), subcutaneously (s.c.; three of four mice), and intraperitoneally (i.p.; one of four mice) immunized groups. Double vaccinations enhanced the anti-tumour effect in all groups except the intravenous (i.v.) group, which failed to achieve complete rejection. The anti-tumour efficacy of each immunization route was correlated with the OVA-specific cytotoxic T lymphocyte (CTL) activity evaluated on day 7 post-vaccination. Furthermore, the accumulation of DC2.4 cells in the regional lymph nodes was detected only in the i.d.-and s.c.-injected groups. These results demonstrate that the administration route of antigen-loaded DCs affects the migration of DCs to lymphoid tissues and the magnitude of antigen-specific CTL response. Furthermore, the immunization route affects vaccine efficiency. © 2001 Cancer Research Campaign http://www.bjcancer.co

Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells

The Gal4/UAS system is a versatile tool to manipulate exogenous gene expression of cells spatially and temporally in many model organisms. Many variations of light-controllable Gal4/UAS system are now available, following the development of photo-activatable (PA) molecular switches and integration of these tools. However, many PA-Gal4 transcription factors have undesired background transcription activities even in dark conditions, and this severely attenuates reliable light-controlled gene expression. Therefore, it is important to develop reliable PA-Gal4 transcription factors with robust light-induced gene expression and limited background activity. By optimization of synthetic PA-Gal4 transcription factors, we have validated configurations of Gal4 DNA biding domain, transcription activation domain and blue light-dependent dimer formation molecule Vivid (VVD), and applied types of transcription activation domains to develop a new PA-Gal4 transcription factor we have named eGAV (enhanced Gal4-VVD transcription factor). Background activity of eGAV in dark conditions was significantly lower than that of hGAVPO, a commonly used PA-Gal4 transcription factor, and maximum light-induced gene expression levels were also improved. Light-controlled gene expression was verified in cultured HEK293T cells with plasmid-transient transfections, and in mouse EpH4 cells with lentivirus vector-mediated transduction. Furthermore, light-controlled eGAV-mediated transcription was confirmed in transfected neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, demonstrating that eGAV can be applied to a wide range of experimental systems and model organisms

Role of mammalian Ecdysoneless in cell cycle regulation.

The Ecdysoneless (Ecd) protein is required for cell-autonomous roles in development and oogenesis in Drosophila, but the function of its evolutionarily conserved mammalian orthologs is not clear. To study the cellular function of Ecd in mammalian cells, we generated Ecd(lox/lox) mouse embryonic fibroblast cells from Ecd floxed mouse embryos. Cre-mediated deletion of Ecd in Ecd(lox/lox) mouse embryonic fibroblasts led to a proliferative block due to a delay in G(1)-S cell cycle progression; this defect was reversed by the introduction of human Ecd. Loss of Ecd led to marked down-regulation of E2F target gene expression. Furthermore, Ecd directly bound to Rb at the pocket domain and competed with E2F for binding to hypophosphorylated Rb. Our results demonstrate that mammalian Ecd plays a role in cell cycle progression via the Rb-E2F pathway