2 research outputs found
Multisensor ImagingFrom Sample Preparation to Integrated Multimodal Interpretation of LA-ICPMS and MALDI MS Imaging Data
Laterally
resolved chemical analysis (chemical imaging) has increasingly
attracted attention in the Life Sciences during the past years. While
some developments have provided improvements in lateral resolution
and speed of analysis, there is a trend toward the combination of
two or more analysis techniques, so-called multisensor imaging, for
providing deeper information into the biochemical processes within
one sample. In this work, a human malignant pleural mesothelioma sample
from a patient treated with cisplatin as a cytostatic agent has been
analyzed using laser ablation inductively coupled plasma mass spectrometry
(LA-ICPMS) and matrix-assisted laser desorption/ionization mass spectrometry
(MALDI MS). While LA-ICPMS was able to provide quantitative information
on the platinum distribution along with the distribution of other
elemental analytes in the tissue sample, MALDI MS could reveal full
information on lipid distributions, as both modes of polarity, negative
and positive, were used for measurements. Tandem MS experiments verified
the occurrence of distinct lipid classes. All imaging analyses were
performed using a lateral resolution of 40 μm, providing information
with excellent depth of details. By analyzing the very same tissue
section, it was possible to perfectly correlate the obtained analyte
distribution information in an evaluation approach comprising LA-ICPMS
and MALDI MS data. Correlations between platinum, phosphorus, and
lipid distributions were found by the use of advanced statistics.
The present proof-of-principle study demonstrates the benefit of data
combination for outcomes beyond one method imaging modality and highlights
the value of advanced chemical imaging in the Life Sciences
Additional file 1: Table S1. of Identification of mumps virus protein and lipid composition by mass spectrometry
Identification of protein bands shown in Figs. 1 & 2. Table S2. List of peptides found in spectra corresponding to NP bands shown in Fig. 1 and possible matching NP and P peptides. Table S3. List of peptides found in spectra corresponding to NP bands shown in Fig. 2 and possible matching NP and P peptides. Figure S1. Peptide mass fingerprint by means of positive ion MALDI reflectron MS of NP forms (depicted in Fig. 2b, reduced sample). Green (bottom) spectrum – NP apparent molecular weight 48 kDa; blue (middle) spectrum – NP apparent molecular weight 55 kDa; red (top) spectrum – NP apparent molecular weight 61 kDa. Full peptide list is given in Table S3. Figure S2. Western blot of mumps virus samples; L-Zg/Vero, JL/Vero, JL/CEF (from left to right) subjected to electrophoresis under reducing denaturating conditions detected by anti-mumps serum. (PDF 612 kb