1 research outputs found
96-Well Plasmonic Sensing with Nanohole Arrays
A multiwell plasmonic reader was
designed and validated for higher
throughput analysis of biological interactions with a platform of
the same size as standard 96-well plates. While the plasmonic sensor
can be read with standard 96-well plate readers, a custom 96-well
plate reader was designed to analyze nanohole arrays at high incident
angles required for higher sensitivity. Gold nanohole arrays were
manufactured on a 4 in. glass wafer using a photolithographic process.
In comparison to single channel measurements with nanohole arrays
fabricated with nanosphere lithography, the nanohole array sensors
greatly enhanced the signal-to-noise ratio of the plasmonic signal
and precision of the measurements with the multiwell plate system.
As proof of concept, the detection of IgG in the low nanomolar range
was achieved with the multiwell plate reader. The multiwell plasmonic
plate reader was also applied to the screening of several prostate
specific (PSA) antibodies for secondary detection of PSA and for the
analysis of an anticancer drug through a competitive assay between
methotrexate (MTX) and folic acid Au nanoparticle (FaNP) for human
dihydrofolate reductase (hDHFR). The multiwell plasmonic reader based
on nanohole array technology offers the rapid, versatile, sensitive,
and simple high throughput detection of biomolecules