NSC130362 binds GSR in a concentration-dependent manner.

<p>(A) GSR and NSC130362 absorbance profile. (B) Absorbance of GSR + NSC130362 in the flow-through after desalting column. (C) <i>inset</i>. The structure of the GSH-NSC10362 adduct. Absorbance of GSR + NSC130362 +GSH in the flow-through after desalting column. All absorbance values in (B and C) were corrected for absorbance of individual NSC130362, GSH, and the GSH-NSC130362 adduct in the flow through. (D) GSR activity in the absence or presence of either NSC130362 or the GSH-NSC130362 adduct. *, <i>P</i> < 0.05.</p

Combined effect of TRAIL and doxorubicin in prostate carcinoma PPC-1 cells.

<p>PPC-1 cells grown to subconfluency in 384 well plate were treated with TRAIL (0.1 ng/ml) and increasing concentrations of doxorubicin. The level of cytotoxicity was determined by an ATPLite reagent. T, TRAIL; Dox. doxorubicin. <i>P</i> < 0.01.</p

Combined treatment of NSC130362 and oxidative stress inducers ATO, Myr, and BSO efficiently induced apoptosis in a variety of cancer cells but not in primary human hepatocytes.

<p>The effect of NSC130362/ATO (A), NSC130362/Myr (B), and NSC130362/BSO (C) combined treatment in breast carcinoma cells and MDA-MB-435 melanoma cells. The effect of NSC130362/ATO and NSC130362/Myr (D) combined treatment in pancreatic, prostate, and lung carcinoma cells as well as in AML cells from cancer patients. Subconfluent cells in a 96-well plate were pre-incubated for 4 h with ATO (3 μM), Myr (100 μM), or BSO (10 μM) followed by treatment with NSC130362 (10 μM) for an additional 24 h. At the end of the treatment, the ratio of dead cells was determined by an ATPLite reagent. *, <i>P</i> < 0.05.</p

Cell surface expression of DR5 and DcR1/DcR2 in MDA-MB-435 carcinoma cells.

<p>MDA-MB-435 cell were pretreated with either ML100 NSC130362 for 4 h and 24 h. Cells were then labeled with biotin and lysed. Labeled cell surface-associated DR5, DcR1, and DcR2 were captured by streptavidin-agarose beads. The precipitated samples were analyzed by Western blotting with the specific respective antibodies and horseradish peroxidase-conjugated secondary antibodies. The level of the cell surface DR4 was below the detection limits. Molecular weight markers are on the left.</p