Thermal constraints on in vivo optogenetic manipulations.

A key assumption of optogenetics is that light only affects opsin-expressing neurons. However, illumination invariably heats tissue, and many physiological processes are temperature-sensitive. Commonly used illumination protocols increased the temperature by 0.2-2 °C and suppressed spiking in multiple brain regions. In the striatum, light delivery activated an inwardly rectifying potassium conductance and biased rotational behavior. Thus, careful consideration of light-delivery parameters is required, as even modest intracranial heating can confound interpretation of optogenetic experiments

Is the cosmic microwave background really non-Gaussian?

Two recent papers have claimed detection of non-Gaussian features in the COBE DMR sky maps of the cosmic microwave background. We confirm these results, but argue that Gaussianity is still not convincingly ruled out. Since a score of non-Gaussianity tests have now been published, one might expect some mildly significant results even by chance. Moreover, in the case of one measure which yields a detection, a bispectrum statistic, we find that if the non-Gaussian feature is real, it may well be due to detector noise rather than a non-Gaussian sky signal, since a signal-to-noise analysis localizes it to angular scales smaller than the beam. We study its spatial origin in case it is nonetheless due to a sky signal (eg, a cosmic string wake or flat-spectrum foreground contaminant). It appears highly localized in the direction b=39.5, l=257, since removing a mere 5 pixels inside a single COBE beam area centered there makes the effect statistically insignificant. We also test Guassianity with an eigenmode analysis which allows a sky map to be treated as a random number generator. A battery of tests of this generator all yield results consistent with Gaussianity.Comment: Revised to match accepted ApJL version. 4 pages with 2 figs included. Links and color fig at http://www.sns.ias.edu/~max/gaussianity_frames.html or from [email protected]

Subtleties of witnessing quantum coherence in non-isolated systems

Identifying non-classicality unambiguously and inexpensively is a long-standing open challenge in physics. The No-Signalling-In-Time protocol was developed as an experimental test for macroscopic realism, and serves as a witness of quantum coherence in isolated quantum systems by comparing the quantum state to its completely dephased counterpart. We show that it provides a lower bound on a certain resource-theoretic coherence monotone. We go on to generalise the protocol to the case where the system of interest is coupled to an environment. Depending on the manner of the generalisation, the resulting witness either reports on system coherence alone, or on a disjunction of system coherence with either (i) the existence of non-classical system-environment correlations or (ii) non-negligible dynamics in the environment. These are distinct failure modes of the Born approximation in non-isolated systems.Comment: 16pp, 2 figs, 5 thms. v2: typos corrected, references added and small change to title to reflect that of published versio