Measurement and verification of zero energy settlements: Lessons learned from four pilot cases in Europe

Measurement and verification (M&V) has become necessary for ensuring intended design performance. Currently, M&V procedures and calculation methods exist for the assessment of Energy Conservation Measures (ECM) for existing buildings, with a focus on reliable baseline model creation and savings estimation, as well as for reducing the computation time, uncertainties, and M&V costs. There is limited application of rigorous M&V procedures in the design, delivery and operation of low/zero energy dwellings and settlements. In the present paper, M&V for four pilot net-zero energy settlements has been designed and implemented. The M&V has been planned, incorporating guidance from existing protocols, linked to the project development phases, and populated with lessons learned through implementation. The resulting framework demonstrates that M&V is not strictly linked to the operational phase of a project but is rather an integral part of the project management and development. Under this scope, M&V is an integrated, iterative process that is accompanied by quality control in every step. Quality control is a significant component of the M&V, and the proposed quality control procedures can support the preparation and implementation of automated M&V. The proposed framework can be useful to project managers for integrating M&V into the project management and development process and explicitly aligning it with the rest of the design and construction procedures

IFN-γ-dependent interactions between tissue-intrinsic γδ T cells and tissue-infiltrating CD8 T cells limit allergic contact dermatitis

BACKGROUND: Elicitation of allergic contact dermatitis (ACD), an inflammatory type 4 hypersensitivity disease, induces skin infiltration by polyclonal effector CD8 αβ T cells and precursors of tissue-resident memory T (TRM) cells. Because TRM have long-term potential to contribute to body-surface immunoprotection and immunopathology, their local regulation needs a fuller understanding.OBJECTIVE: We sought to investigate how TRM-cell maturation might be influenced by innate-like T cells pre-existing within many epithelia.METHODS: This study examined CD8+ TRM-cell maturation following hapten-induced ACD in wild-type mice and in strains harboring altered compartments of dendritic intraepidermal γδ T cells (DETCs), a prototypic tissue-intrinsic, innate-like T-cell compartment that reportedly regulates ACD, but by no elucidated mechanism.RESULTS: In addition to eliciting CD8 TRM, ACD induced DETC activation and an intimate coregulatory association of the 2 cell types. This depended on DETC sensing IFN-γ produced by CD8 cells and involved programmed death-ligand 1 (PD-L1). Thus, in mice lacking DETC or lacking IFN-γ receptor solely on γδ cells, ACD-elicited CD8 T cells showed enhanced proliferative and effector potentials and reduced motility, collectively associated with exaggerated ACD pathology. Comparable dysregulation was elicited by PD-L1 blockade in vitro, and IFN-γ-regulated PD-L1 expression was a trait of human skin-homing and intraepithelial γδ T cells.CONCLUSIONS: The size and quality of the tissue-infiltrating CD8 T-cell response during ACD can be profoundly regulated by local innate-like T cells responding to IFN-γ and involving PD-L1. Thus, interindividual and tissue-specific variations in tissue-intrinsic lymphocytes may influence responses to allergens and other challenges and may underpin inflammatory pathologies such as those repeatedly observed in γδ T-cell-deficient settings.</p

Measurement and verification of Zero Energy settlements: lessons learned from four pilot cases in Europe

Summarization: Measurement and verification (M&V) has become necessary for ensuring intended design performance. Currently, M&V procedures and calculation methods exist for the assessment of Energy Conservation Measures (ECM) for existing buildings, with a focus on reliable baseline model creation and savings estimation, as well as for reducing the computation time, uncertainties, and M&V costs. There is limited application of rigorous M&V procedures in the design, delivery and operation of low/zero energy dwellings and settlements. In the present paper, M&V for four pilot net-zero energy settlements has been designed and implemented. The M&V has been planned, incorporating guidance from existing protocols, linked to the project development phases, and populated with lessons learned through implementation. The resulting framework demonstrates that M&V is not strictly linked to the operational phase of a project but is rather an integral part of the project management and development. Under this scope, M&V is an integrated, iterative process that is accompanied by quality control in every step. Quality control is a significant component of the M&V, and the proposed quality control procedures can support the preparation and implementation of automated M&V. The proposed framework can be useful to project managers for integrating M&V into the project management and development process and explicitly aligning it with the rest of the design and construction procedures.Presented on: Sustainabilit