24 research outputs found
Association between the number of glutamine (Gln) repetitions and healthy/pathologic state in 114 canine blood and MCT samples.
<p>Pearson χ<sup>2</sup> test (p = 0.3454; not significant).</p
List of genetic variations grouped for gene, relative population frequency and allelic frequencies in the MCT cohort of samples.
<p>List of genetic variations grouped for gene, relative population frequency and allelic frequencies in the MCT cohort of samples.</p
List of genetic variations detected in the glutamine rich region of TET2 exon 11 with relative population frequency and total glutamine residues number in the 75 MCT samples.
<p>List of genetic variations detected in the glutamine rich region of TET2 exon 11 with relative population frequency and total glutamine residues number in the 75 MCT samples.</p
Forward (F) and reverse (R) primer sequences of canine genes included in the present study and used for polymerase chain reaction with the corresponding annealing temperature and product length.
<p>Forward (F) and reverse (R) primer sequences of canine genes included in the present study and used for polymerase chain reaction with the corresponding annealing temperature and product length.</p
List of target genes and percentage of protein sequence identity between dog and other reference species (<i>Homo sapiens</i>, <i>Felis catus</i>, <i>Mus musculus</i>, <i>Rattus norvegicus</i>).
<p>NA: sequence not available in the databases.</p><p>List of target genes and percentage of protein sequence identity between dog and other reference species (<i>Homo sapiens</i>, <i>Felis catus</i>, <i>Mus musculus</i>, <i>Rattus norvegicus</i>).</p
Sequence alignment between dog, cat, human, mouse and rat specific glutamine-rich regions located in exon 3 and 11 of TET2 gene.
<p>The image was obtained using the tool ClustalW2 (<a href="http://www.ebi.ac.uk/Tools/msa/clustalw2/" target="_blank">http://www.ebi.ac.uk/Tools/msa/clustalw2/</a>).</p
The canine kit2 sequences show distinct chiroptical features.
<p>CD titrations of 4 µM canine (d_kit2 and d_kit2_A16 in Panels A and B, respectively) and human (h_kit2 in Panel C) sequences with increasing concentrations of KCl (0–60 mM) in 10 mM Tris pH 7.5, 25°C. In Panel D the relative variations of the d_kit2_A16 CD signal recorded at 260 and 295 as a function of metal ion concentration are reported.</p
The human and canine kit1 sequences share a common G-4 fold.
<p>CD titrations of µM d_kit1 (Panel A) or h_kit1 (Panel B) with increasing concentrations of KCl (0–200 mM) in 10 mM Tris, pH 7.5, 25°C. The induction of G-4 folded form was supported by TDS (Panel C) and quantified from the variation of the CD signal at 260 nm (Panel D).</p
Melting temperatures of the tested sequences determined from the analysis of the variation of the CD signal recorded at 260°C/min was applied.
<p>*values determined at 295 nm.</p
The identified (SNP) in canine kit2 is not associated to the healthy/MCT state.
<p>Association between the presence of d_kit2 or d_kit2_A16 and healthy/pathologic state in 51 canine blood and tumor samples. Fisher exact test (p = 0.1577).</p