Extended analysis of intratumoral heterogeneity of primary osteosarcoma tissue using 3D-in-vivo-tumor-model

BACKGROUND: Osteosarcomas are a rare, heterogeneous and malignant group of bone tumors that have a high potential for metastasis and aggressive growth patterns. Treatment of metastasized osteosarcoma is often insufficient and research is compromised by problems encountered when culturing cells or analyzing genetic alterations due to the high level of intratumoral and intertumoral heterogeneity. The chick chorioallantoic membrane (CAM) model, a 3D-in-vivo-tumor-model, could potentially facilitate the investigation of osteosarcoma heterogeneity at an individual and highly specified level. OBJECTIVE: Objective was to establish the grafting and transplantation of different primary osteosarcoma tissue parts onto several consecutive CAMs for tumor profiling and investigation of osteosarcoma heterogeneity. METHODS: Various parts of primary osteosarcoma tissue were grafted onto CAMs and were transplanted onto another CAM for five to seven consecutive times, enabling further experimental analyzes. RESULTS: Primary osteosarcoma tissue parts exhibited satisfactory growth patterns and displayed angiogenic development on the CAM. It was possible to graft and transplant different tumor parts several times while the tissue viability was still high and tumor profiling was performed. CONCLUSIONS: Primary osteosarcoma tissue grew on several different CAMs for an extended time period and neovascularization of serial transplanted tumor parts was observed, improving the versatility of the 3D-in-vivo-tumor-model

ASEAN Orthopaedic Association Education Committee - Orthopaedic curriculum for residency training 2019

In November 2013, Dr Dohar Tobing, who was the President of the ASEAN Orthopaedic Association at that time, organized a Seminar on Orthopaedic Education in Yogyakarta to discuss residency training in ASEAN. At this initial meeting the training program in each country was presented by representatives from each ASEAN country. Subsequently the ASEAN Orthopaedic Association established the Education Committee under the chairmanship of Professor Lee Eng Hin to develop a common syllabus/curriculum for all ASEAN countries based on minimum standards required to practice competently as a general orthopaedic surgeon. The main objective of having a common curriculum is to facilitate exchanges of residents and fellows amongst the ASEAN countries to increase their exposure to orthopaedic conditions in the region and improve their breadth and depth of knowledge and skills. The first meeting of the Education Committee was held in Cebu in November 2014 and the Committee has since met twice a year in May and November. Training programs in the ASEAN countries were discussed and a consensus was reached on the structure and length of the training program for the AOA curriculum. In May 2016, three workgroups were formed under the leadership of Professor David Choon, Dr Dohar Tobing and Professor Lee Eng Hin to develop the detailed curriculum in the following areas: a) General Orthopaedics/Trauma/Infection/Sports/Pathophysiology/Basic Science Lead: David Choon Members: Arturo C. Canete, Jean Pierre F. Leung, Apipop Kritsaneephaiboon, Maung Mg Htwe, Sabarul A. Mokhtar b) Adult Orthopaedics/Spine/Upper Limb/Foot & Ankle/Biomaterials/Biomechanics Lead: Dohar Tobing Members: Myint Thaung, Peter Lee, Yeo Sing Jin, Kamarul Ariffin Khalid, Khin Mg Myint, Azlina Abbas c) Pediatrics/Oncology Lead: Lee Eng Hin Members: Edward HM Wang, Kanyika Chamniprasas, Pornchai Mulpruek, Istan Irmansyah Irsan, Abdul Razak Sulaiman, Wan Faisham Nu’man Wan Ismail In addition to their contributions as workgroup members, the following individuals made substantial contributions in the following areas: Pathophysiology/Basic Science: Vivek Ajit Singh and David Choon General Ortho: Wan Faisham Nu’man Wan Ismail and Kamarul Ariffin Khalid Trauma: Arturo Cañete and Jean Pierre Leung Sports Medicine: Aung Myo Win Adult-Hip & Knee: Maung Mg Htwe and Myint Thaung Spine: Dohar Tobing Upper limb/Hand: Khin Mg Myint Foot & Ankle: Inderjeet Singh Paediatrics: Lee Eng Hin and Abdul Razak Sulaiman Oncology: Edward Wang and Istan Irmansyah Irsa