Obtention de gel par application de hautes pressions à un concentré de protéines de lait

Obtention de gel par application de hautes pressions à un concentré de protéines de lait. congrès annuel du GF

Newborn calf intestinal absorption of immunoglobulins extracted from bovine colostrum.

O objetivo desta pesquisa consistiu em estudar a absorção de imunoglobulinas G (IgG) extraídas do colostro por ultrafiltração e recolocadas em solução salina (IECRSS). Cinqüenta bezerros holandeses Holstein Frisian (ME) recém-nascidos (41 machos e 9 fêmeas) foram distribuídos ao acaso em cinco tratamentos (dez animais em cada tratamento) com a seguinte composição: a. colostro; b. IECRSS + caseino-macropeptídeo (CMP), c. IECRSS + ácido isobutírico; d. IECRSS + extrato liofilizado de colostro, e e. IECRSS pura. O colostro e as IECRSS (à base de 44 g/l de IgG) foram distribuídos à razão de 25 g/kg FV. Os complementos (CMP, ácido isobutírico e extrato liofilizado de colostro) foram incorporados na proporção de: 100, 125, 250 mg/kg PV, respectivamente. Os animais foram alimentados com colostro ou IECRSS + complemento, duas, dez e dezoito horas após o nascimento. As coletas de sangue foram efetuadas antes da ingestão do colostro ou IECRSS, bem como às 26 horas de vida. A temperatura corporal foi medida antes de cada coleta de sangue. A seguir, determinou-se a taxa de hematócrito. Os níveis de IgG foram dosados a partir do plasma, por nefelometija. As IECRSS foram absorvidas em proporções menores em comparação ao colostro in natura, independentemente da natureza do complemento adicionado (PC 0,01). Os níveis de IgG alcançados a 26 horas de vida foram (média ± erro padrão da média): 5,12 ± 0,79; 6,74 ± 0,82; 7,61 ± 1,18; 7,58 ± 0,85 e 14,9 ± 1,39 (g/l), para os tratamentos C, B, E, D e A, respectivamente. A taxa de hematrócrito e a temperatura corporal não foram influenciadas (P >0,05) pelos tratamentos, independentemente da ordem de coleta (entre 0 e 26 h). Conclui-se que as IECRSS, independentemente da natureza do complemento adicionado, não são absorvidas eficazmente como as imunoglobulinas 6 do colostro in natura.The aim of the present work is to study the absorption of immunoglobulins G extracted from colostrum and administered in a saline solution (IECRSS). Fifty (41 male and 9 female) newborn Holstein calves were randomly divided into five groups of ten animals each. Calves were guiven ether a. colostrum; b. IECRSS + CMP; c. IECRSS + isobutyric acid; d. IECRSS + freeze-dried colostrum extract, and e- pure IECRS5. Colostrum and IECRSS, each of them containing 44 g/l of IgG, were given at a level of 25 g/kg body weight. The complements (CMP, isobutyric acid and colostrumextract) were added at the levels of 100. 125 and 250 mg/kg body weight, respectively. Blood samples were taken just before the meals as well as 26 h after birth. The animals were fed on colostrum or IECRSS + + compliement two, ten and eighteen hours after birth. Blood was sampled before ingestion of colostrum or IECRSS and 26 hoursafter birth. Body temperature was measured betore each blood sampling and therafter the hematocrit level was determined. The IgG levels were dosed up from plasma by nephelometry. Compared with that of colostrum IgG in nature, IECRSS absorption was reduced independently of the added complement (P C 0.01). The plasma levels of IgG at 26 hours after birth were 5.12 ± 0.79; 6.74 ±0.82:7.61 ± 1.18; 7.58 ± 0.85 and 14.9 ± 139 (g/l) in calves of groups A, B, C, D, E respectively. The hematocrit level and the body temperature were not influenced (P >0.05) by the treatments, independently of the blood collection (between 0 and 26 hours). It was concluded that the IECRSS, independently of the nature of the added complement, are less efficiently absorted than the IgG of colostrum in natura

Liquid milk products: Membrane-processed liquid milk

 Mention d'édition : 2ème éditionLiquid milk products: Membrane-processed liquid mil