Microarray-based analysis of recombinant protein production in E.coli

The production of heterologous proteins in E. coli is a powerful tool in the generation of many important biotechnological and medical products. Despite its widespread use as an expression host, however, yields of correctly folded, functional protein are frequently low in E. coli. This is due largely to the formation of insoluble protein aggregates and to premature lysis of the bacterial cells. We, and others, have previously shown that the cell lysis phenomenon associated with recombinant protein production in E. coli is not a direct result of synthesis of heterologous proteins [1], [2]. Instead, protein production triggers a global stress response in the bacterium, but the mechanism by which cell lysis subsequently occurs remains unclear [3]

Additional file 1: of LowMACA: exploiting protein family analysis for the identification of rare driver mutations in cancer

It contains detailed results of our analyses in Figures S1-S4. (PDF 1180 kb

Additional file 2: of methylPipe and compEpiTools: a suite of R packages for the integrative analysis of epigenomics data

Comparison of DMRs identified using various tools contains the source code and the results of a comparative analysis of the DMRs identified using various tools. (PDF 429 kb

Additional file 1: of methylPipe and compEpiTools: a suite of R packages for the integrative analysis of epigenomics data

methylPipe and compEpiTools supplemental vignette illustrating a workflow built on publicly available DNA methylation and epigenomics data, where a number of the provided features from both packages are exemplified. (PDF 1156 kb

Additional file 2: of LowMACA: exploiting protein family analysis for the identification of rare driver mutations in cancer

It contains detailed results of our analyses in Table S5 and S6. (XLSX 232 kb