Additional file 1 of Fc-engineered monoclonal antibodies to reduce off-target liver uptake

Additional file 1. Figure S1: Example of mass-analysis of mAbs conjugated with DFO. Analysis of the immunoconjugate was performed by Maldi-tof (Rapiflex, Bruker) to determine the number of DFO conjugated to mAbs. Peaks of the entire antibody are indicated by an arrow. Figure S2: Binding of D4a WT Fc and D4a LALAPG Fc after DFO conjugation on cancer cells by flow cytometry. Anti-AXL antibody D4a with WT Fc and with LALAPG mutations were used at 5 μg/ml to stain AXL positive cell line for 1h at 4°c in PBS-BSA buffer. After 3 wash, a secondary fluorescent labeled anti-hFc mAbs labeled was used to reveal binding of D4a mAbs at the cell surface of the cell