Clinical and electrocardiographic characteristics of study patients.

<p>CE clinical event; F female; ICD implantable cardioverter defibrillator; LQTS long-QT syndrome; M male; n number; ns not significant; TWAR T-wave amplitude range; TWI T-wave integral. Values are expressed as mean ± SD where applicable.</p

Receiver operating characteristic curve analysis with T-wave integral of the key channel 60 (blue lines; AUC 0.89), T-wave amplitude range of the key channel 90 (red lines; AUC 0.83), T_peak-T_end-interval in standard chest lead V₁ (black lines; AUC 0.8) and QTc time from standard ECG (green line; AUC 0.86).

<p>Receiver operating characteristic curve analysis with T-wave integral of the key channel 60 (blue lines; AUC 0.89), T-wave amplitude range of the key channel 90 (red lines; AUC 0.83), T_peak-T_end-interval in standard chest lead V₁ (black lines; AUC 0.8) and QTc time from standard ECG (green line; AUC 0.86).</p

Superimposition of two typical BSM recordings from the region of interest: Red curves from a patient with LQT2 and without clinical event, black curves from a patient with LQT2 and with clinical event.

<p>Region of interest for T-wave integral is yellow highlighted, region of interest for T-wave amplitude range is blue highlighted. Channel numbers are listed on the left top of each rectangle, Area under curve values from ROC analysis are listed on the left bottom and P values of ROC analysis are listed on the right bottom of each rectangle</p

Array of 120 electrodes for the recording of unipolar body surface potentials.

<p>Red rectangles indicate the positions of standard chest leads V1 to V6. Red frame indicates the region of interest of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158085#pone.0158085.g003" target="_blank">Fig 3</a>.</p

Results of genetic analysis, beta-blocker dosage and clinical events of our study patients; # = patients with clinical event.

<p>Results of genetic analysis, beta-blocker dosage and clinical events of our study patients; # = patients with clinical event.</p

Subject Characteristics.

<p>The table shows demographics of the study population. Continuous variables are expressed as mean ± standard deviation. Volumes were adjusted to body surface area. BMI: Body mass index, ESV: endsystolic volume, EDV: enddiastolic volume, SV: stroke volume, LV: left ventricle</p><p>Subject Characteristics.</p

Relationship between strain values of the experienced and the inexperienced observer.

<p>Results show the strain values for (Ecc_endo) of the experienced and the inexperienced observer according to the visual reference standard as defined by the experienced observers reading.</p

1493delK mutant and wild-type (WT) human cardiac sodium channel current expressed in HEK293 cells.

<p>(A) Whole-cell sodium current traces in response to increasing step depolarizations in WT (left) and 1493delK (right). (B) Voltage protocols for activation and steady-state inactivation. (C) Averaged sodium current– voltage relation for WT and 1493delK sodium channels. (D) Bar histogram showing averaged WT and 1493delK sodium peak currents at −20 mV. (E) Average voltage-dependence of activation and steady-state inactivation for wild-type (WT) and 1493delK sodium channels. For the activation curve, normalized peak conductance was plotted as a function of the membrane potential. For the inactivation curve, peak sodium currents were normalized to maximum values in each cell and plotted as a function of the voltage of the conditioning step.</p

Clinical and Genetic Characteristics of <i>SCN5A</i> 1493delK Carriers.

<p>ECG, electrocardiogram; ICD, implantable cardioverter defibrillator; PM, Pacemaker; m, male; f, female; y, years; +, mutation carrier or ICD implanted; (+),obligate mutation carrier; SCD, sudden cardiac death; RV, right ventricle; CAD, coronary artery disease; #, by transthoracic echocardiographic; §, by magnetic resonance imaging; $, by ventriculography; n.p., not performed; n.a., not avalible; PWD, P-wave duration; AVB I°, atrioventricular block first-degree; CCD, cardiac conduction delay; RFB, right fascicular block; LAFB, left anterior fascicular block. Propositus (10021_149) printed in italics.</p

Contour Tracking in Short-Axis Orientation.

<p>The figure shows a representative example of the tracking in Short-Axis orientation of an ischemically damaged left ventricle (LV) at basal, midventricular and apical levels. The initial contour is set in end-diastole (left side of the rows) and after application of the feature tracking algorithm the contours (based on the underlying 48 control points) are automatically detected over the cardiac cycle.</p