Radioimmunoassay specifications.

<p>Note. n/a  =  not assessed in these tissue types.</p

Steroid concentrations in plasma, whole blood, and brain.

<p>Corticosterone and DHEA levels in plasma, whole blood, and brain (caudal telencephalon, cTel) of sham-perfused subjects. *p≤0.05, ***p≤0.001.</p

Brain steroid concentrations after saline or sham perfusion.

<p>(<b>A</b>) Corticosterone, (<b>B</b>) DHEA, (<b>C</b>) testosterone, and (<b>D</b>) 17β-estradiol concentrations were measured in male zebra finch brain regions after saline perfusion or sham perfusion (control). Rostral telencephalon  =  rTel, caudal telencephalon  =  cTel, diencephalon  =  Dien, cerebellum  =  Cb. *p≤0.05, **p≤0.01.</p

Steroid concentrations in plasma and whole blood before and after sham perfusion.

<p>(<b>A</b>) Corticosterone and (<b>B</b>) DHEA levels in plasma and whole blood at baseline (<3 min) and after sham perfusion (∼11 min) (corticosterone n = 9 baseline, 5 post-sham perfusion; DHEA n = 8 baseline, 3 post-sham perfusion). Baseline blood samples were from the brachial vein, post sham-perfusion blood samples were from the heart. *p≤0.05, ***p≤0.001, #p = 0.06.</p