Quality magazine : improving your manufacturing process

<p>Venn diagrams of differentially expressed genes, where <b>(A)</b> green circles indicate number of total genes are up-regulated each time point, and <b>(B)</b> red circles indicate number of total genes were down-regulated each time point. EE indicates cells analyzed in early exponential phase, ME, mid-exponential phase and ES, early stationary phase, where actual times of sampling each cell population is indicated in the methods as well as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0186440#pone.0186440.g001" target="_blank">Fig 1A</a>. <b>(C)</b> Changes in the gene profile as a result of lignin exposure in early exponential phase, mid-exponential and early stationary phase of growth. In the right side of each chart (blue), number of genes with increased relative abundance, and in the left side (red), number of genes with lower relative abundance. The genes are grouped according to functional class as defined by COG annotation.</p

Total genes up and down regulated in each of the three points monitored during growth of SCF1 in lignin-amended cultures.

<p>The number in parenthesis indicates the percentage of the genes that were differentially expressed under each time.</p

Summary of genome attributes of <i>Enterobacter lignolyticus</i> SCF1 and three close relatives, <i>E</i>. <i>aerogenes</i>, <i>E</i>. <i>cloacae</i> ATCC13047, and <i>Enterobacter</i> sp. 638.

<p>Summary of genome attributes of <i>Enterobacter lignolyticus</i> SCF1 and three close relatives, <i>E</i>. <i>aerogenes</i>, <i>E</i>. <i>cloacae</i> ATCC13047, and <i>Enterobacter</i> sp. 638.</p

Clustering of transcripts shows response to lignin in genes of interest in <i>Enterobacter lignolyticus</i> SCF1 grown in glucose with and without lignin.

<p>Fold change indicates change in relative abundance of transcript in lignin-amended compared to unamended growth. Colors to the left of the heatmap indicate functional annotations as indicated. Each row represents gene expression of cells grown in lignin and glucose, compared to cells grown in glucose alone at the same time point.</p