Kainic acid induces expression of caveolin-1 in activated microglia in rat brain

Caveolin-1, a major constituent of caveolae, has been implicated in endocytosis, signal transduction and cholesterol transport in a wide variety of cells. In the present study, the expression of caveolin-1 was examined by immunohistochemistry in rat brain with or without systemic injection of kainic acid (KA). Caveolin-1 immunoreactivity was observed in capillary walls in brains of control rats. From one to seven days after KA injection, caveolin-1 immunoreactivity appeared in activated microglia in the cerebral cortex, hippocampus and other brain regions. The strongest immunoreactivity of microglia was seen after 3 days after KA administration. The expression of caveolin-1 was confirmed by RT-PCR and Western blot analysis, respectively. The induction of caveolin-1 expression in microglia activated in response to kainic acid administration suggests its possible role in a modulation of inflammation. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 25–30

タンイツ ノ コクシツ ドーパミン シンケイ サイボウ ワ センジョウタイ ニ オイテ コウハンイ カツ コウミツドナ ジクサク ブンプ オ ケイセイスル

京都大学0048新制・論文博士博士(医学)乙第12324号論医博第1981号新制||医||975(附属図書館)UT51-2009-D539京都大学大学院医学研究科脳統御医科学系専攻(主査)教授 大森 治紀, 教授 福山 秀直, 教授 髙橋 良輔学位規則第4条第2項該当Doctor of Medical ScienceKyoto UniversityDA

Pseudotumor cerebri and lung cancer-associated jugular vein thrombosis: Role of anatomical variations of torcular herophili

A 71-year-old male appeared at the facility complaining of disturbance of consciousness and bilateral papilledema. The laboratory test revealed anemia and coagulation abnormality. A physical examination and magnetic resonance imaging (MRI) of the brain with and without gadolinium showed no abnormalities. A lumbar puncture showed a high pressure, but a normal cerebrospinal fluid (CSF) cell count. Cerebral angiography showed no morphological abnormalities, but it revealed an asymmetric right dominant type of confluence of the sinuses with the partially-communicating left transverse sinus in the late phase. Furthermore, there was a delay in the cerebral circulation time (CCT). Subsequently, venography and ultrasonography revealed right internal jugular vein thrombosis associated with lung cancer. The patient recovered from the disturbance of consciousness immediately after an emergency ventriculoperitoneal shunt and anticoagulation therapy. This case was diagnosed as secondary pseudotumor cerebri (PTC). In order to facilitate the early detection of secondary PTC, it is important to take note of symptoms of intracranial hypertension with no remarkable intracranial lesions and to consider the possibility of PTC, especially in the patients with high risk factors for coagulopathy including lung cancer. Keywords: Cerebral circulation time, Confluence of sinuses (torcular Herophili), Idiopathic intracranial hypertension, Jugular vein thrombosis, Lung cancer, Pseudotumor cerebr

Difference in the expression of CIRBP, RBM3 and HSP70 in the myocardium and cerebellum after death by hypothermia and carbon monoxide poisoning

Abstract: We studied the expression of hypoxia-related antigens (e.g., cold-inducible antigens and apoptotic antigens) in the myocardium and the cerebellumthat were obtained from individuals after death by carbon monoxide or hypothermia. The immunohistochemistry results revealed that expression of cold-inducible RNA binding protein (CIRBP) and RNA-binding protein 3 (RBM3) may be associated with hpyothermic and the hypoxic conditions. The expression of CIRBP and RBM3 in the myocardium was different from their expression in the cerebellum, especially in the Purkinje cells. The results indicate that agonal duration influences antigen expression. In the hypothermic condition, the myocardium uses more ATP since the force of the excitation-contraction coupling of the myocardium increases by more than 400% when the experimental temperature is reduced from 35°C to 25°C. The results obtained in this study indicate that physicians should pay attention to the myocardium when cooling the patient's body to protect the brain

Axonal arborization of dopaminergic neurons of the rat midbrain:mechanism of Parkinson\u27s disease

科学研究費補助金研究成果報告書研究種目: 基盤研究(C)研究期間: 2010～2012課題番号: 22500307研究代表者: 松田 和郎（滋賀医科大学・医学部・助教）研究分担者: 安原 治（滋賀医科大学・医学部・客員教授）研究分担者: 本間 智（滋賀医科大学・医学部・助教）研究分担者: 宇田川 潤（滋賀医科大学・医学部・教授