2,693 research outputs found
Genes for selenium dependent and independent formate dehydrogenase in the gut microbial communities of three lower, wood-feeding termites and a wood-feeding roach
The bacterial Wood-Ljungdahl pathway for CO_2-reductive acetogenesis is important for the nutritional mutualism occurring between
wood-feeding insects and their hindgut microbiota. A key step in this
pathway is the reduction of CO_2 to formate, catalysed by the enzyme
formate dehydrogenase (FDH). Putative selenocysteine- (Sec) and
cysteine- (Cys) containing paralogues of hydrogenase-linked FDH (FDH_H)
have been identified in the termite gut acetogenic spirochete,
Treponema primitia, but knowledge of their relevance in the termite gut
environment remains limited. In this study, we designed degenerate PCR
primers for FDH_H genes (fdhF) and assessed fdhF diversity in insect gut
bacterial isolates and the gut microbial communities of termites and
cockroaches. The insects examined herein represent three wood-feeding
termite families, Termopsidae, Kalotermitidae and Rhinotermitidae
(phylogenetically 'lower' termite taxa); the wood-feeding roach family
Cryptocercidae (the sister taxon to termites); and the omnivorous roach
family Blattidae. Sec and Cys FDH_H variants were identified in every
wood-feeding insect but not the omnivorous roach. Of 68 novel alleles
obtained from inventories, 66 affiliated phylogenetically with enzymes
from T. primitia. These formed two subclades (37 and 29 phylotypes)
almost completely comprised of Sec-containing and Cys-containing
enzymes respectively. A gut cDNA inventory showed transcription of both
variants in the termite Zootermopsis nevadensis (family Termopsidae).
The gene patterns suggest that FDH_H enzymes are important for the
CO_2-reductive metabolism of uncultured acetogenic treponemes and imply
that the availability of selenium, a trace element, shaped microbial
gene content in the last common ancestor of dictyopteran, wood-feeding
insects, and continues to shape it to this day
Infrared observations of asteroids from earth and space
Infrared reflectances at wavelength between 1 and 4 micrometers are used for determining asteroid surface mineralogy, surface composition, diameters, and albedos. Thermal models were developed for analyzing infrared observations at longer wavelengths. The discovery of a spectral feature due to water of hydration on Ceres seems to contradict the mineralogy inferred from spectrophotometry
IRAS asteroid families
The Infrared Astronomical Satellite (IRAS) sampled the entire asteroid population at wavelengths from 12 to 100 microns during its 1983 all sky survey. The IRAS Minor Planet Survey (IMPS) includes updated results for more recently numbered as well as other additional asteroids with reliable orbital elements. Albedos and diameters were derived from the observed thermal emission and assumed absolute visual magnitudes and then entered into the IMPS database at the Infrared Processing and Analysis Center (IPAC) for members of the Themis, Eos, Koronis and Maria asteroid families and compared with their visual colors. The IMPS results for the small (down to about 20 km) asteroids within these major families confirm trends previously noted for their larger members. Each of these dynamical families which are defined by their similar proper elements appears to have homogeneous physical properties
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Exploring Anasazi Origins: The Cedar Mesa Basketmaker II
In 1990 the Cedar Mesa Project II (CMP II) was initiated to further explore the origins of the Anasazi tradition on Cedar Mesa, southeast Utah. Research focused on the Basketmaker II occupation of the mesa. As the initial members of the Anasazi tradition, now represented by the modern Pueblo Indians, the origins of the Basketmaker II remains a topic of debate
Cell cycle proliferation decisions: the impact of single cell analyses
Cell proliferation is a fundamental requirement for organismal development and homeostasis. The mammalian cell division cycle is tightly controlled to ensure complete and precise genome duplication and segregation of replicated chromosomes to daughter cells. The onset of DNA replication marks an irreversible commitment to cell division, and the accumulated efforts of many decades of molecular and cellular studies have probed this cellular decision, commonly called the restriction point. Despite a long-standing conceptual framework of the restriction point for progression through G1 phase into S phase or exit from G1 phase to quiescence (G0), recent technical advances in quantitative single cell analysis of mammalian cells have provided new insights. Significant intercellular heterogeneity revealed by single cell studies and the discovery of discrete subpopulations in proliferating cultures suggests the need for an even more nuanced understanding of cell proliferation decisions. In this review, we describe some of the recent developments in the cell cycle field made possible by quantitative single cell experimental approaches
Development of improved thermoelectric mater- ials for spacecraft applications final summary report, 29 jun. 1964 - 29 jun. 1965
Thermoelectric materials for spacecraft applications - optimization of bismuth-antimony alloys and ag-sb-fe-te-se system alloys for thermoelectric cooling in space environmen
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Genome-Wide Effects of Selenium and Translational Uncoupling on Transcription in the Termite Gut Symbiont Treponema primitia
When prokaryotic cells acquire mutations, encounter translation-inhibiting substances, or experience adverse environmental conditions that limit their ability to synthesize proteins, transcription can become uncoupled from translation. Such uncoupling is known to suppress transcription of protein-encoding genes in bacteria. Here we show that the trace element selenium controls transcription of the gene for the selenocysteine-utilizing enzyme formate dehydrogenase (fdhF_Sec) through a translation-coupled mechanism in the termite gut symbiont Treponema primitia, a member of the bacterial phylum Spirochaetes. We also evaluated changes in genome-wide transcriptional patterns caused by selenium limitation and by generally uncoupling translation from transcription via antibiotic-mediated inhibition of protein synthesis. We observed that inhibiting protein synthesis in T. primitia influences transcriptional patterns in unexpected ways. In addition to suppressing transcription of certain genes, the expected consequence of inhibiting protein synthesis, we found numerous examples in which transcription of genes and operons is truncated far downstream from putative promoters, is unchanged, or is even stimulated overall. These results indicate that gene regulation in bacteria allows for specific post-initiation transcriptional responses during periods of limited protein synthesis, which may depend both on translational coupling and on unclassified intrinsic elements of protein-encoding genes.
A large body of literature demonstrates that the coupling of transcription and translation is a general and essential method by which bacteria regulate gene expression levels. However, the potential role of noncanonical amino acids in regulating transcriptional output via translational control remains, for the most part, undefined. Furthermore, the genome-wide transcriptional state in response to translational decoupling is not well quantified. The results presented here suggest that the noncanonical amino acid selenocysteine is able to tune transcription of an important metabolic gene via translational coupling. Furthermore, a genome-wide analysis reveals that transcriptional decoupling produces a wide-ranging effect and that this effect is not uniform. These results exemplify how growth conditions that impact translational processivity can rapidly feed back on transcriptional productivity of prespecified groups of genes, providing bacteria with an efficient response to environmental changes
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