Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry

Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32

Identification of isolated viral strains of atypical avian influenza using molecular methods of virological diagnostics

In addition to the implementation of standard methods of virological diagnostics used for the isolation of the Newcastle disease virus from suspect material, as well as for its identification, nowadays there is increasing use of molecular diagnostic methods, primarily reverse transcription polymerase chain reaction (RT-PCR) and the sequencing method. The objective of this work was to examine possibilities for the implementation of the above methods in the diagnosis of poultry infection caused by the Newcastle disease virus. The presence of hem agglutination antigens for the Newcastle disease virus was established in samples of allantoises liquid from 62 poultry embargoed eggs 72 h after inoculation, whose titers ranged from 1:16 to 1:2048, while the hem agglutination inhibition test (HI test) with the implementation of a referent immuno serum against the given cause provided the identification of isolated viruses in serum dilutions of 1:128 to 1:1024. The RT-PCR method and the PCR established that in eight examined samples one fragment each of viral RNA is formed in agars gel of a size of 254bp, which is characteristic for the Newcastle disease virus genome according to its nucleotide sequence. On the grounds of a comparative analysis of RNA sequences obtained from eight isolated NDV strains and the genome sequences of referent atypical poultry influenza viral strains using Mega 40 and BLAST programmers, it was established that the isolated strains of the Newcastle disease virus were highly virulent

Uporedno ispitivanje osetljivosti i specifičnosti imunoenzimske probe i testa inhibicije hemaglutinacije u serološkoj dijagnostici newcastle bolesti živine

Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.Uporedno ispitivanje osetljivosti i specifičnosti indirektne imunoenzimske probe - iELISA i testa inhibicije hemaglutinacije (HI testa) u serološkoj dijagnostici Newcastle bolesti živine vršeno je korišćenjem uzoraka krvnog seruma nevakcinisane i vakcinisane živine. Primenom imunoenzimske probe - iELISA ukupno je ispitano četrnaest uzoraka krvnog seruma nevakcinisane živine, od kojih je devet bilo pozitivno na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, dok su dva uzorka bila sumnjiva, a kod tri uzorka nije ustanovljeno prisustvo specifičnih antitela. Ispitivanjem 82 uzorka krvnog seruma vakcinisane živine na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, utvrđeno je njihovo prisustvo kod 80 uzoraka seruma, dok je jedan uzorak bio sumnjiv, a jedan negativan. Vrednosti titra specifičnih antitela u uzorcima krvnog seruma vakcinisane i nevakcinisane živine ustanovljene primenom testa inhibicije hemaglutinacije (HI testa), kretale su se od 1:2 do 1:32

Volatiles of Thymus serpyllum Obtained by Three Different Methods

Volatiles of Thymus serpyllum L. were obtained by hydrodistillation (HD), simultaneous hydrodistillation, and extraction (SDE) and static headspace gas chromatography-mass spectrometry analysis (head space [HS]), respectively. Monoterpenes were the most dominant in all 3 techniques (84.8%-94.2%). Essential oil profiles obtained by HD and SDE were similar, with oxygenated monoterpenes being the most abundant (up to 75.4%). In HS volatiles of T. serpyllum monoterpene hydrocarbons strongly dominated (94.2%). The main compounds were α-terpinyl acetate (HD and SDE) and myrcene (HS)

Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry

Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.Uporedno ispitivanje osetljivosti i specifičnosti indirektne imunoenzimske probe - iELISA i testa inhibicije hemaglutinacije (HI testa) u serološkoj dijagnostici Newcastle bolesti živine vršeno je korišćenjem uzoraka krvnog seruma nevakcinisane i vakcinisane živine. Primenom imunoenzimske probe - iELISA ukupno je ispitano četrnaest uzoraka krvnog seruma nevakcinisane živine, od kojih je devet bilo pozitivno na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, dok su dva uzorka bila sumnjiva, a kod tri uzorka nije ustanovljeno prisustvo specifičnih antitela. Ispitivanjem 82 uzorka krvnog seruma vakcinisane živine na prisustvo specifičnih antitela protiv virusa Newcastle bolesti, utvrđeno je njihovo prisustvo kod 80 uzoraka seruma, dok je jedan uzorak bio sumnjiv, a jedan negativan. Vrednosti titra specifičnih antitela u uzorcima krvnog seruma vakcinisane i nevakcinisane živine ustanovljene primenom testa inhibicije hemaglutinacije (HI testa), kretale su se od 1:2 do 1:32

Etarsko ulje četina duglazije (pseudotsuga menziesii mirb. Franco) iz različitih provenijencija

The essential oils of the fresh neddles from different provenances of Dougles fir (Pseudosuga menziesii) were analysed by GC and GC-MS. Monoterpenes, a-pinene, camphene, b-pinene, citronenal and bornyl acetate were identified by mass spectra and retention time correlations, as major constituents. The differences in the percentages of terpene components indicated division of 6 populations in two groups, one of them richer in a-pinene, camphene, and bornyl acetate and the other type in a-pinene, b-pinene and citronenal.Metodama gasne hromatografije (GH) i kombinacije gasne hromarografija-masene spektrometrije (GH/MS) ispitivan je kvalitativni i kvantitativni sadržaj etarskog ulja jednogodišnjih i dvogodišnjih četina duglazije (Pseudotstuga menziesii Mirb. Franco) različitih provinijencija

Etarsko ulje četina duglazije (pseudotsuga menziesii mirb. Franco) iz različitih provenijencija

The essential oils of the fresh neddles from different provenances of Dougles fir (Pseudosuga menziesii) were analysed by GC and GC-MS. Monoterpenes, a-pinene, camphene, b-pinene, citronenal and bornyl acetate were identified by mass spectra and retention time correlations, as major constituents. The differences in the percentages of terpene components indicated division of 6 populations in two groups, one of them richer in a-pinene, camphene, and bornyl acetate and the other type in a-pinene, b-pinene and citronenal.Metodama gasne hromatografije (GH) i kombinacije gasne hromarografija-masene spektrometrije (GH/MS) ispitivan je kvalitativni i kvantitativni sadržaj etarskog ulja jednogodišnjih i dvogodišnjih četina duglazije (Pseudotstuga menziesii Mirb. Franco) različitih provinijencija