3 research outputs found
Phylogenetic relationship among Kenyan sorghum germplasms based on aluminium tolerance
Eighty nine (89) sorghum lines sourced from various parts of Kenya were used to determine phylogenetic relationships based on 10 DNA fragments at AltSB loci with SbMATE, ORF9 and MITE primers. Nine lines of varying aluminium tolerance levels were selected to compare their SbMATE gene expression via the real-time PCR quantification of SbMATE gene expression. The sorghum line MSCR O2 expressed a thousandfold more SbMATEgene activity than the sensitive lines (MSCRM49, MSCRN84 and MSCRN61) under Al treatment. Analysis was done by agarose gel electrophoresis stained with ethidium bromide. The objective of this study was to assess the level of phylogenetic relationships among the Kenyan sorghum germplasms at a known Al tolerance locus. Hierarchical cluster analysis joined at 70% simple matching coefficient using average linkage similarity level produced nine groups in which 67 lines fell in three major clusters of 39, 15 and 13 lines each. The three Al tolerant lines MSCRO2, MSCRC1 and MSCRN60 were clustered together. Lines MSCRO2, MSCRC1 and MSCRN60, screened to be Al tolerant were genetically related at 70% average linkage similarity level and therefore recommend their further development as a food security measure in Kenya.Keywords: Aluminium (Al) toxicity, Sorghum bicolor, Sorghum bicolor multi-drug, toxic extrusion compound (SbMATE) gene expressionAfrican Journal of Biotechnology Vol. 12(22), pp. 3528-353
Cheprot et Al_PHYSIOLOGICAL CHARACTERIZATION OF KENYAN SORGHUM LINES.pdf
Eighty nine Kenyan sorghum lines were screened for tolerance to aluminium toxicity in nutrient
solution. Relative net root growth; root tip aluminium content and variation in organic acid
exudation were used to determine the tolerance or sensitivity of the sorghum lines at 148 μM Al for
six days. The lines showed variable reduction in root growth under the Al stress. On the basis of
the relative net root growths, three lines were tolerant, nineteen were moderately tolerant and sixty
seven were sensitive to the Al stress. The tolerant lines secreted up to five times more citrate
compared to sensitive lines under the Al treatment. All the lines secreted extremely low quantities
of malate under aluminium stress despite a significant positive regression (R 2 = 0.83) between
malate secretion and relative net root growth. There was a negative regression between relative
net root growth and root aluminium concentration (R2 = -0.79) among the selected sorghum lines,
and the sensitive lines accumulated up to three times the amount of Al compared to the tolerant
lines.. The Al tolerant sorghum lines were selected for improved sorghum production in acid soil.
The objectives of this study were to (i) identify Al tolerant Kenyan sorghum lines, (ii) investigate
tolerance mechanisms employed by Kenyan sorghum lines against Al stress
Phylogenetic Relationship among Kenyan Sorghum_Cheprot et al 2013.pdf
Eighty nine (89) sorghum lines sourced from various parts of Kenya were used to determine
phylogenetic relationships based on 10 DNA fragments at AltSB loci with SbMATE, ORF9 and MITE
primers. Nine lines of varying aluminium tolerance levels were selected to compare their SbMATE gene
expression via the real-time PCR quantification of SbMATE gene expression. The sorghum line MSCR
O2 expressed a thousandfold more SbMATEgene activity than the sensitive lines (MSCRM49, MSCRN84
and MSCRN61) under Al treatment. Analysis was done by agarose gel electrophoresis stained with
ethidium bromide. The objective of this study was to assess the level of phylogenetic relationships
among the Kenyan sorghum germplasms at a known Al tolerance locus. Hierarchical cluster analysis
joined at 70% simple matching coefficient using average linkage similarity level produced nine groups
in which 67 lines fell in three major clusters of 39, 15 and 13 lines each. The three Al tolerant lines
MSCRO2, MSCRC1 and MSCRN60 were clustered together. Lines MSCRO2, MSCRC1 and MSCRN60,
screened to be Al tolerant were genetically related at 70% average linkage similarity level and therefore
recommend their further development as a food security measure in Kenya