STORAGE OF ACTIVITY OF THE HUMAN CHORIONIC GONADOTROPIN HORMONE IN SOLUTION AT ADDITION OF ORGANIC COMPOUNDS TO THE PHARMACOLOGICAL COMPOSITION

The more stable among the tested samples were samples with saccharose in the concentration of 50–75 mg per cm3. While adding of L- lysine to samples the most stable activity was discovered in the experimental series of samples with the content of lysine of 10 mg per cm3 – activity increased by 54 % as compared to theoretical initial activity of HCG during 8 weeks. While storing gonadotropin with L-glycine fluctuations of hormone activity in all series of samples were observed. Adding of 0.2 mg per cm3 of L-glycine had a more expressed stabilizing effect. Adding of 0.2 mg per cm3 of L-methionine produced relatively high and stable activity of gonadotropin during the 6 weeks storage. Adding of 0.25 mg / cm3 of L- glycine and 75.50 mg / cm3 of saccharose to experimental samples during 2 weeks at 40 °C provided 69.8 % and 60.7 % saving activity of hCG respectively. Activity of gonadotropin in a series of samples with the addition of L- glycine and mannitol was significantly lower and at the end of the study was at an appropriate rate with the control series models. The highest activity of gonadotropin was detected while adding fillers – 10 mg / cm3 L-lysine and 75 mg / cm3 saccharose and mannitol – to recipes as a stabilizer

STORAGE OF ACTIVITY OF THE HUMAN CHORIONIC GONADOTROPIN HORMONE IN SOLUTION AT ADDITION OF ORGANIC COMPOUNDS TO THE PHARMACOLOGICAL COMPOSITION

The more stable among the tested samples were samples with saccharose in the concentration of 50–75 mg per cm3. While adding of L- lysine to samples the most stable activity was discovered in the experimental series of samples with the content of lysine of 10 mg per cm3 – activity increased by 54 % as compared to theoretical initial activity of HCG during 8 weeks. While storing gonadotropin with L-glycine fluctuations of hormone activity in all series of samples were observed. Adding of 0.2 mg per cm3 of L-glycine had a more expressed stabilizing effect. Adding of 0.2 mg per cm3 of L-methionine produced relatively high and stable activity of gonadotropin during the 6 weeks storage. Adding of 0.25 mg / cm3 of L- glycine and 75.50 mg / cm3 of saccharose to experimental samples during 2 weeks at 40 °C provided 69.8 % and 60.7 % saving activity of hCG respectively. Activity of gonadotropin in a series of samples with the addition of L- glycine and mannitol was significantly lower and at the end of the study was at an appropriate rate with the control series models. The highest activity of gonadotropin was detected while adding fillers – 10 mg / cm3 L-lysine and 75 mg / cm3 saccharose and mannitol – to recipes as a stabilizer

STABILIZING THE GONADOTROPIN ACTIVITY WITH THE USE OF DIFFERENT ORGANIC COMPOUNDS

Complex studies of optimum quantitative and qualitative composition of carbohydrates and amino acids necessary for gonadotropines activity stabilization were the aim of our investigations. This was determined by biological active substances - saccharose, L- lysine which stabilizes gonadotropins activity for longer use while preserving activity. As a result of the studies we found out that during 6 weeks of incubation at 40 0 ɋ, the activity of hCG was the highest in the samples with 50 and 75 mg/sm3 of saccharose. Concentration of the hormone was high at 8 week of the incubation in the group of samples with 75 mg/sm3 of saccharose. In the series of hCG samples with 10 mg/sm3 of L - lysine, the activity of hormone was 54% during 8 weeks. Compatible adding of 10 mg/cm3 of L-lysine + 75 mg/sm3 of saccharose provided the best stabilization gonadotropin activity