Clinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing.

Severe acute respiratory syndrome coronavirus 2 is an RNA virus currently causing a pandemic. Due to errors during replication, mutations can occur and result in cell adaptation by the virus or in the rise of new variants. This can change the attachment receptors' usage, result in different morphology of plaques, and can affect as well antiviral development. Indeed, a molecule can be active on laboratory strains but not necessarily on circulating strains or be effective only against some viral variants. Experiments with clinical samples with limited cell adaptation should be performed to confirm the efficiency of drugs of interest. In this protocol, we present a method to culture severe acute respiratory syndrome coronavirus 2 from nasopharyngeal swabs, obtain a high viral titer while limiting cell adaptation, and assess antiviral efficiency

Cosmological Luminosity Evolution of QSO/AGN Population

We apply the observed optical/X-ray spectral states of the Galactic black hole candidates (GBHCs) to the cosmological QSO luminosity evolution under the assumptions that QSOs and GBHCs are powered by similar accretion processes and that their emission mechanisms are also similar. The QSO luminosity function (LF) evolution in various energy bands is strongly affected by the spectral evolution which is tightly correlated with the luminosity evolution. We generate a random sample of QSOs born nearly synchronously by allowing the QSOs to have redshifts in a narrow range around an initial high redshift, black hole masses according to a power-law, and mass accretion rates near Eddington rates. The QSOs evolve as a single long-lived population on the cosmological time scale. The pure luminosity evolution results in distinct luminosity evolution features due to the strong spectral evolution. Most notably, different energy bands (optical/UV, soft X-ray, and hard X-ray) show different evolutionary trends and the hard X-ray LF in particular shows an apparent reversal of the luminosity evolution (from decreasing to increasing luminosity) at low redshifts, which is not seen in the conventional pure luminosity evolution scenario without spectral evolution. The resulting mass function of black holes (BHs), which is qualitatively consistent with the observed QSO LF evolution, shows that QSO remnants are likely to be found as BHs with masses in the range 10**8-5x10**10 solar masses. The long-lived single population of QSOs are expected to leave their remnants as supermassive BHs residing in rare, giant elliptical galaxies.Comment: 9 pages, 2 figures, ApJ

The ESO-Sculptor Faint Galaxy Redshift Survey: The Photometric Sample

We present the photometric sample of a faint galaxy survey carried out in the southern hemisphere, using CCDs on the 3.60m and NTT-3.5m telescopes at La Silla (ESO). The survey area is a continuous strip of 0.2 deg x 1.53 deg located at high galactic latitude (-83 deg) in the Sculptor constellation. The photometric survey provides total magnitudes in the bands B, V (Johnson) and R (Cousins) to limiting magnitudes of 24.5, 24.0, 23.5 respectively. To these limits, the catalog contains about 9500, 12150, 13000 galaxies in B, V, R bands respectively and is the first large digital multi-colour photometric catalog at this depth. This photometric survey also provides the entry catalog for a fully-sampled redshift survey of ~ 700 galaxies with R < 20.5 (Bellanger et al. 1995). In this paper, we describe the photometric observations and the steps used in the data reduction. The analysis of objects and the star-galaxy separation with a neural network are performed using SExtractor, a new photometric software developed by E. Bertin (1996). The photometric accuracy of the resulting catalog is ~ 0.05 mag for R < 22. The differential galaxy number counts in B, V, R are in good agreement with previously published CCD studies and confirm the evidence for significant evolution at faint magnitudes as compared to a standard non evolving model (by factors 3.6, 2.6, 2.1). The galaxy colour distributions B-R, B-V of our sample show a blueing trend of ~ 0.5 mag between 21 < R < 23.5 in contrast to the V-R colour distribution where no significant evolution is observed.Comment: LATEX, 18 Postscript figures, 20 pages. To appear July 1997. Modified version of article. Abstract corrected for missing lin

Pure Luminosity Evolution Hypothesis for QSOs: From Luminosity Functions to Synthetic Catalogues

This paper describes the simulation of realistic Monte-Carlo extragalactic catalogues, aimed at comparing the behaviour of cosmological tests versus input parameters. QSO catalogues are built on a Luminosity Function derived from data through suitable computation of individual maximum volumes in complete (but magnitude- and redshift-limited) samples requiring neither of redshift nor of apparent magnitude histogram. The values of the evolution parameter are derived for various cosmologies, corresponding to =1/2 in the sample of 400 Ultra-Violet Excess (UVX) QSOs (Boyle et al 1990). The various luminosity functions are compared, both for the whole sample and in redshift bins. An evolution characteristic time is defined and computed, depending strongly on the cosmology, but practically constant when expressed in terms of the age of the Universe. Algorithms are given for producing unbiased or biased catalogues based on the null hypothesis that the objects are uniformly distributed in volume but suffer Pure Luminosity Evolution.Comment: uuencode compressed tar file of Latex and macros files. Tar compressed poscript files of the papers and figures are also available by anonymous ftp at ftp://summer.obs-mip.fr/pub/OUTGOING/paper2 or upon request at [email protected]

The 8 bits 100 MS/s Pipeline ADC for the INNOTEP Project – TWEPP-09

This paper describes the Analog to Digital Converter developed for the front end electronic of the IN2P3 INNOTEP project by the “pole microelectronique Rhone-Auvergne”. (Collaboration between LPC Clermont-Ferrand and IPNL Lyon). This ADC is a 4 stages 2.5 bits per stage pipe line with open loops track and holds and amplifiers. It runs at 100MSamples/s and has 8 bits resolution. The stages used two lines, the gain line and the comparison line, with most operators running in current. The main idea of this current line is to make a first step toward an all in current structure. Currently, this ADC is designed with a 0,35μm SiGe technology