100 research outputs found
Superior mesenteric artery syndrome following initiation of cisplatin-containing chemotherapy: a case report
Hemiparasitism effect on Baccharis dracunculifolia DC. and consequences to its major galling herbivore
Evaluation of the anti-inflammatory and analgesic effects of green tea (Camellia sinensis) in mice
Is there a therapeutic window for pentoxifylline after the onset of acute pancreatitis?
Physicochemical and biological characterization of 1E10 Anti-Idiotype vaccine
<p>Abstract</p> <p>Background</p> <p>1E10 monoclonal antibody is a murine anti-idiotypic antibody that mimics N-glycolyl-GM3 gangliosides. This antibody has been tested as an anti-idiotypic cancer vaccine, adjuvated in Al(OH)<sub>3</sub>, in several clinical trials for melanoma, breast, and lung cancer. During early clinical development this mAb was obtained <it>in vivo </it>from mice ascites fluid. Currently, the production process of 1E10 is being transferred from the <it>in vivo </it>to a bioreactor-based method.</p> <p>Results</p> <p>Here, we present a comprehensive molecular and immunological characterization of 1E10 produced by the two different production processes in order to determine the impact of the manufacturing process in vaccine performance. We observed differences in glycosylation pattern, charge heterogeneity and structural stability between <it>in vivo</it>-produced 1E10 and bioreactor-obtained 1E10. Interestingly, these modifications had no significant impact on the immune responses elicited in two different animal models.</p> <p>Conclusions</p> <p>Changes in 1E10 primary structure like glycosylation; asparagine deamidation and oxidation affected 1E10 structural stability but did not affect the immune response elicited in mice and chickens when compared to 1E10 produced in mice.</p
PROPOSAL OF A CLINICAL CARE PATHWAY FOR THE MANAGEMENT OF ACUTE UPPER GASTROINTESTINAL BLEEDING
Cimento ósseo com gentamicina no tratamento da infecção óssea: estudo da eluição in vitro
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