Représentation de la réussite scolaire et professionnelle : homogénéité et différenciation en fonction du sexe et de l'origine sociale d'étudiants en lettres et sciences humaines

Jamet G., Massonnat Jean, Mathet M. Représentation de la réussite scolaire et professionnelle : homogénéité et différenciation en fonction du sexe et de l'origine sociale d'étudiants en lettres et sciences humaines. In: Bulletin de psychologie, tome 35 n°353, 1981. Psychologie de l'éducation. pp. 242-256

X-ray absorption analysis of sputter-grown Co/Pt stackings before and after helium irradiation

We have studied the local order around cobalt atoms in Pt/Co/Pt layered systems gradually modified by irradiation using 30 keV helium ions. Using X-ray diffraction and X-ray Absorption Fine Structure, we have determined the crystallographic order, the number of Co-Co and Co-Pt bonds and the corresponding bond lengths both in-plane and in the perpendicular direction. The comparison with an interdiffusion model highlights the unexpected complexity of the initial Pt/Co/Pt nanostructure. We use the Néel/Bruno model of magnetic anisotropy to correlate the structure and the magnetic hysteresis properties. We then identify the structural consequences of irradiation onto the short range order. The irradiation induces a substitutional mixing maintaining the initial crystallographic structure. We confirm that the mixing rate is in agreement with a ballistic mechanism of mixing. In addition, we show that the previously reported irradiation-induced controlled decrease of the magnetic anisotropy can not be solely attributed to Co-Pt intermixing: irradiation also significantly releases the 3.4% tensile strain of the cobalt-rich dense planes. We finally speculate on the class of magnetic materials the concept of light ion irradiation could be extended to

Dissimilar expression of multidrug resistance mdr1 and bcrp by the replication of hepatitis C virus: Role of the non structural 5A protein.

Multidrug resistance associated with the overexpression of ATP-dependent binding cassette (ABC) proteins is widely accepted as an important cause of treatment failure in patients with neoplastic or infectious diseases. Some of them play also a pivotal role in detoxification processes. Herein, we investigated the effect of hepatitis C virus (HCV) replication and nonstructural 5A (NS5A) protein on the expression and functional activity of two ABC transport proteins: MDR1 and BCRP. RT-quantitative real-time polymerase chain reaction (qPCR) was carried out for mdr1 and bcrp mRNAs in both Huh7 cells expressing NS5A and Huh7.5 cells containing either full-length- or subgenomic-HCV replicon systems. The functional activity of these pumps was studied by performing a dye efflux assay with DiOC2 and Rhodamine 123. A dose-dependent down-regulation of mdr1 expression was documented in Huh7 cells expressing the NS5A protein, as well as in both replicon systems. In contrast, a significant increase of bcrp expression in both systems was recorded, which were in full agreement with the dye efflux assay results. These results warrant further in vivo studies in HCV patients with cholestasis and/or patients that are refractive to the pharmacotherapy due to the activity of these pumps

L’ichtyose du Golden retriever due à une mutation de la PNPLA1 est associée à une accumulation de lipides neutres et de phospholipides dans le Stratum Corneum (SC)

National audienceno abstrac

Development of an alternative method for testing the immunogenicity of diphtheria vaccines

The immunogenicity of the diphtheria component of 73 commercial vaccines from five different manufacturers was tested by the toxin neutralization test (TNT) and the enzyme-linked immunosorbent assay (ELISA) developed in our laboratory. A comparison of the antibody levels measured by both assays showed a very good correlation (r = 0.95, p < 0.001). The results suggest that the proposed ELISA is a reliable, simple and economical alternative to the TNT in guinea pigs. Also, the ELISA was found to measure IgG antibody levels as low as 5.5 x 10(-5) IU ml-1. To evaluate the possibility of accelerating the active immunization during the activity test of vaccines, an alternative schedule using one single human dose was assayed. A very good correlation was observed between the IgG antibody response obtained with this schedule and with the traditional programme. Therefore, the cost and the time required to perform the activity test may be considerably reduced when both the rapid immunization schedule and the ELISA are used

X-ray absorption analysis of sputter-grown Co/Pt stackings before and after helium irradiation

PACS. 61.10.Ht X-ray absorption spectroscopy: EXAFS, NEXAFS, XANES, etc. – 61.82.Bg Metals and alloys – 75.30.Gw Magnetic anisotropy,

Development of an ELISA for measuring the activity of tetanus toxoid in vaccines and comparison with the toxin neutralization test in mice

An enzyme-linked immunosorbent assay (ELISA) has been developed to measure anti-tetanus toxoid antibody levels in immunized guinea-pig sera as a useful alternative to the currently used toxin neutralization test (TNT) in determining the activity of the tetanus toxoid in vaccines. The ELISA was found to measure antibody levels as low as 5.8 x 10(-5) IU/ml. Furthermore, a comparison of the results from ELISA and TNT involving 132 different commercial vaccines showed a very good correlation (r = 0.94, p < 0.001) between antibody levels measured by both methods. The results suggest that the proposed ELISA is a reliable, simple and economical alternative to the TNT in mice for assessing the activity of tetanus toxoids in vaccines