12 research outputs found
Analyse und (Weiter-)Entwicklung von Umsetzungsmechanismen und Politikansätzen zur Stärkung und Aufwertung des Deutschen Ressourceneffizienzprogramms ProgRess : Abschlussbericht
Living conditions and autonomy levels in COPD patients receiving non-invasive ventilation: impact on health related quality of life
Background!#!Research on health-related quality of life (HRQL) has become increasingly important in recent decades. However, the impact of both living conditions and the level of autonomy impairments on HRQL in COPD patients receiving non-invasive ventilation (NIV) is still unclear.!##!Methods!#!The Severe Respiratory Insufficiency Questionnaire (SRI) was used to measure HRQL in a prospective cohort of COPD patients in whom home NIV was already established. Data on sociodemographics, clinical characteristics and standardized levels of autonomy impairment were evaluated. A multiple linear regression analysis was performed to identify the factors associated with a reduced HRQL.!##!Results!#!A total of 137 patients (67.0 ± 7.8 years, 45% female) were assessed. The mean SRI Summary Score was 54.1 ± 16.9 (95%CI: 51.1-57.1; N = 127). Regular ambulatory care was provided in 76% of patients, but only 37% underwent pulmonary rehabilitation. Overall, 69% of patients lived with family members, while 31% lived alone (family situation). Autonomy impairment levels were most serious in 3%, serious in 14%, and significant in 29% of patients, while 54% had no impairments at all. Of note, higher levels of autonomy impairment were markedly associated with lower SRI scores (regression coefficient - 6.5 ± 1.1 per level; P < 0.001). In contrast, family situation (0.2 ± 3.0; P = 0.959), ambulatory care by a respiratory specialist (1.7 ± 3.6; P = 0.638), and pulmonary rehabilitation (- 0.8 ± 3.1; P = 0.802) did not appear to influence HRQL. Possible subgroup effects were evident for the factors 'impaired autonomy' and 'living in a nursing home' (P = 0.016).!##!Conclusion!#!A higher level of autonomy impairment has been identified as the major determinant of reduced HRQL in COPD-patients receiving long-term NIV, particularly in those living in a nursing home. Trial Registration German Clinical Trials Register (DRKS00008759)
The use of digital web-based video training for correct inhalation technique during the COVID-19 pandemic
An expanded LUXendin color palette for GLP1R detection and visualization in vitro and in vivo
The glucagon-like peptide-1 receptor (GLP1R) is expressed in peripheral tissues and the brain, where it exerts pleiotropic actions on metabolic and inflammatory processes. Detection and visualization of GLP1R remains challenging, partly due to a lack of validated reagents. Previously, we generated LUXendins, antagonistic red and far-red fluorescent probes for specific labeling of GLP1R in live and fixed cells/tissue. We now extend this concept to the green and near-infrared color ranges by synthesizing and testing LUXendin492, LUXendin551, LUXendin615 and LUXendin762. All four probes brightly and specifically label GLP1R in cells and pancreatic islets. Further, LUXendin551 acts as chemical beta cell reporter in preclinical rodent models, while LUXendin762 allows non-invasive imaging, highlighting differentially-accessible GLP1R populations. We thus expand the color palette of LUXendins to seven different spectra, opening up a range of experiments using widefield microscopy available in most labs through super-resolution imaging and whole animal imaging. With this, we expect that LUXendins will continue to generate novel and specific insight into GLP1R biology
Sulfonated red and far-red rhodamines to visualize SNAP- and Halo-tagged cell surface proteins
The (in)ability to permeate membranes is a key feature of chemical biology probes that defines
their suitability for specific applications. Here we report sulfonated rhodamines that endow
xanthene dyes with cellular impermeability for analysis of surface proteins. We fuse charged
sulfonates to red and far-red dyes to obtain Sulfo549 and Sulfo646, respectively, and further link
these to benzylguanine and choloralkane substrates for SNAP-tag and Halo-tag labelling.
Sulfonated rhodamine-conjugated fluorophores maintain desirable photophysical properties,
such as brightness and photostability. While transfected cells with a nuclear localized SNAP-tag
remain unlabelled, extracellular exposed tags can be cleanly visualized. By multiplexing with a
permeable rhodamine, we are able to differentiate extra- and intracellular SNAP- and Halo-tags,
including those installed on the glucagon-like peptide-1 receptor, a prototypical class B G proteincoupled receptor. In more complex biological systems, Sulfo549 and Sulfo646 labelled
transfected neurons derived from induced pluripotent stem cells (iPSCs), allowing STED
nanoscopy of the axonal membrane. Together, this work provides a new avenue for rendering
dyes impermeable for exclusive extracellular visualization via self-labelling protein tags. We
anticipate that Sulfo549, Sulfo646 and their congeners will be useful for a number of cell biology
applications where labelling of intracellular sites interferes with accurate surface protein analysis
Expanded LUXendin color palette for GLP1R detection and visualization in vitro and in vivo
[Image: see text] The glucagon-like peptide-1 receptor (GLP1R) is expressed in peripheral tissues and the brain, where it exerts pleiotropic actions on metabolic and inflammatory processes. Detection and visualization of GLP1R remains challenging, partly due to a lack of validated reagents. Previously, we generated LUXendins, antagonistic red and far-red fluorescent probes for specific labeling of GLP1R in live and fixed cells/tissues. We now extend this concept to the green and near-infrared color ranges by synthesizing and testing LUXendin492, LUXendin551, LUXendin615, and LUXendin762. All four probes brightly and specifically label GLP1R in cells and pancreatic islets. Further, LUXendin551 acts as a chemical beta cell reporter in preclinical rodent models, while LUXendin762 allows noninvasive imaging, highlighting differentially accessible GLP1R populations. We thus expand the color palette of LUXendins to seven different spectra, opening up a range of experiments using wide-field microscopy available in most labs through super-resolution imaging and whole animal imaging. With this, we expect that LUXendins will continue to generate novel and specific insights into GLP1R biology
Sulfonated red and far-red rhodamines to visualize SNAP- and Halo-tagged cell surface proteins
The (in)ability to permeate membranes is a key feature of chemical biology probes that defines
their suitability for specific applications. Here we report sulfonated rhodamines that endow
xanthene dyes with cellular impermeability for analysis of surface proteins. We fuse charged
sulfonates to red and far-red dyes to obtain Sulfo549 and Sulfo646, respectively, and further link
these to benzylguanine and choloralkane substrates for SNAP-tag and Halo-tag labelling.
Sulfonated rhodamine-conjugated fluorophores maintain desirable photophysical properties,
such as brightness and photostability. While transfected cells with a nuclear localized SNAP-tag
remain unlabelled, extracellular exposed tags can be cleanly visualized. By multiplexing with a
permeable rhodamine, we are able to differentiate extra- and intracellular SNAP- and Halo-tags,
including those installed on the glucagon-like peptide-1 receptor, a prototypical class B G proteincoupled receptor. In more complex biological systems, Sulfo549 and Sulfo646 labelled
transfected neurons derived from induced pluripotent stem cells (iPSCs), allowing STED
nanoscopy of the axonal membrane. Together, this work provides a new avenue for rendering
dyes impermeable for exclusive extracellular visualization via self-labelling protein tags. We
anticipate that Sulfo549, Sulfo646 and their congeners will be useful for a number of cell biology
applications where labelling of intracellular sites interferes with accurate surface protein analysis