Prevalence of Periapical Lesions Observed in Panoramic Radiographs

O presente trabalho teve como objetivo fazer um levantamento epidemiológico da prevalência de lesões periapicais radiolúcidas numa amostra de 107 radiografias panorâmicas, de pacientes atendidos no Curso de Graduação em Odontologia da Faculdade Meridional/IMED. Foram incluídos pacientes que possuíam radiografias panorâmicas realizadas no ano de 2015 e com o termo de Consentimento Livre e Esclarecido devidamente assinado. A coleta de dados foi feita através dos prontuários bem como, laudos e radiografias panorâmicas. Os critérios avaliados foram: presença de lesão periapical visível radiograficamente, gênero do paciente, idade, número total de dentes, elemento dentário, arcada e número de tratamentos endodônticos. Os dados foram organizados e comparados. Os resultados demonstraram que 53,3% dos laudos radiográficos apresentaram lesão periapical. A maioria das lesões encontrava-se em maxila, com 58,5% das ocorrências e os elementos dentários mais acometidos foram o 22 e 26. Concluiu-se que as lesões periapicais foram mais frequentes em mulheres na arcada superior e nos dentes incisivos laterais e primeiros molares superiores. The present study had as objective to perform an epidemiological survey on radiolucent periapical lesions prevalence in a sample of 107 panoramic radiographs of patients in the graduation course in Dentistry school IMED. Patients included have panoramic radiographs performed in the year 2015 and with the term of Free and Informed Consent signed. The data collection regarded panoramic reports exams and radiographs. The criteria was: periapical lesion presence visible in radiographic, patient’s gender, age, total number of teeth, dental element, arch and number of endodontic treatments. The data were organized and compared. The results showed that 53.3% of the radiographic reports had a periapical lesion. Most of the lesions were in the maxilla, with 58.5% of the occurrences and the most affected dental elements were 22 and 26. In conclusion, periapical lesions were more frequent in women in the upper arch on lateral incisors and upper first molars

Reference genes for proximal femoral epiphysiolysis expression studies in broilers cartilage.

The use of reference genes is required for relative quantification in gene expression analysis and the stability of these genes can be variable depending on the experimental design. Therefore, it is indispensable to test the reliability of endogenous genes previously to their use. This study evaluated nine candidate reference genes to select the most stable genes to be used as reference in gene expression studies with the femoral cartilage of normal and epiphysiolysis-affected broilers. The femur articular cartilage of 29 male broilers with 35 days of age was collected, frozen and further submitted to RNA extraction and quantitative PCR (qPCR) analysis. The candidate reference genes evaluated were GAPDH, HMBS, HPRT1, MRPS27, MRPS30, RPL30, RPL4, RPL5, and RPLP1. For the gene stability evaluation, three software were used: GeNorm, BestKeeper and NormFinder, and a global ranking was generated using the function RankAggreg. In this study, the RPLP1 and RPL5 were the most reliable endogenous genes being recommended for expression studies with femur cartilage in broilers with epiphysiolysis and possible other femur anomalies