1 research outputs found
Effect of Ingested Tungsten Oxide (WO<sub><i>x</i></sub>) Nanofibers on Digestive Gland Tissue of Porcellio scaber (Isopoda, Crustacea): Fourier Transform Infrared (FTIR) Imaging
Tungsten nanofibers
are recognized as biologically potent. We study
deviations in molecular composition between normal and digestive gland
tissue of WO<sub><i>x</i></sub> nanofibers (nano-WO<sub><i>x</i></sub>) fed invertebrate Porcellio
scaber (Iosopda, Crustacea) and revealed mechanisms
of nano-WO<sub><i>x</i></sub> effect <i>in vivo</i>. Fourier Transform Infrared (FTIR) imaging performed on digestive
gland epithelium was supplemented by toxicity and cytotoxicity analyses
as well as scanning electron microscopy (SEM) of the surface of the
epithelium. The difference in the spectra of the Nano-WO<sub><i>x</i></sub> treated and control cells showed up in the central
region of the cells and were related to lipid peroxidation, and structural
changes of nucleic acids. The conventional toxicity parameters failed
to show toxic effects of nano-WO<sub><i>x</i></sub>, whereas
the cytotoxicity biomarkers and SEM investigation of digestive gland
epithelium indicated sporadic effects of nanofibers. Since toxicological
and cytological measurements did not highlight severe effects, the
biochemical alterations evidenced by FTIR imaging have been explained
as the result of cell protection (acclimation) mechanisms to unfavorable
conditions and indication of a nonhomeostatic state, which can lead
to toxic effects