Biodegradation of anthracene by a newly isolated bacterial strain, Bacillus thuringiensis AT .ISM .1, isolated from a fly ash deposition site

The current study is aimed to evaluate the mechanism of anthracene degradation by a bacterial strain isolated from fly ash deposition site near Jamadoba Coal Preparation Plant, Jharkhand, India. The Bushnell‐Haas media cultured (containing anthracene as sole carbon source) bacterial isolate was identified by 16S rRNA gene sequence coding as the Bacillus thuringiensis strain, which showed the efficiency to degrade anthracene. The degradation efficiency of the strain has been estimated to be around 91% (for 40 mg l−1 of anthracene concentration) after 2 weeks of incubation at 33–36°C and initial pH of 6•8–7. The growth kinetics of the isolated strain has been described well by the Haldane–Andrews model of microbial growth pattern for inhibitory substrate, with a correlation factor (R 2 value) of 0•9790. The maximum specific growth rate (μ max) was 0•01053 h−1 and the value of inhibition coefficient for Haldane model was specified as 18•2448 mg l−1. In the present study, some diphenol metabolites were identified besides the known possible biodegradation products

Indoor Quality of Residential Homes and Schools of an Industrial Area in Asansol: Characterization, Bioaccessibility and Health Risk Assessment of Potentially Toxic Elements

Bioaccessibility of eight potentially toxic elements (PTEs), their human exposure and health risk assessments were determined in the indoor dust of residence and schools from the Asansol Industrial area, India. The PTEs concentrations were maximum during the winter both at houses and schools. The average PTEs concentrations throughout the year in Asansol were 3.16, 120, 156, 41708, 2354, 61.3, 115 and 345 mg.kg-1 for Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn respectively. X-ray powder diffraction indicated an abundance of quartz in the indoor dust. Principal component analysis (PCA) indicated multiple sources such as traffic, industries, and lithogenic sources for PTEs in indoor dust. Percentage of bioaccessibility was maximum for Cd (55.3% throughout the year). Total PTEs concentration and a bioaccessible fraction of PTEs both were used for health risk assessment, and non-carcinogenic health risk was <1 for total PTEs and the bioaccessible fraction of PTEs. Health risk of total PTEs’ (HItotal) for Mn was high for both children and adult (6.76E-01 and 1.3E-01, respectively). Monte Carlo simulation model indicated that all the cumulative probability of Hazard Quotient (HQ) for collectively eight metals was below 1

Potassium Influencing Physiological Parameters, Photosynthesis and Sugarcane Yield in Subtropical India

Potassium plays an important role in the metabolism of plants, growth, development and yields. Worldwide, KCl is commonly used as source of K fertilizer in agricultural crops. The present experiment was taken up to assess the response of various sources of K on physiological attributes, yield and quality parameters of sugarcane. Treatments were no K application, potassium ammonium sulphate (H4KNO4S-9:0:30:20, NPKS ratio), slow release K fertilizer (0:0:45:0), sulphate of potash/potassium sulphate (K2SO4-0:0:50:17.5), muriate of potash (KCl-0:0:50:0) and muriate of potash (KCl) + elemental S @ 40 kg ha−1. Application of K2SO4 recorded 15.8% improvement in tiller population (during peak tillering stage) and 22.2% improvement in dry matter accumulation (at the harvest stage) over the KCl. About 12.33–24.16% increments in crop growth rate and the highest net photosynthetic rate (19.91 µmol m−2 s−1) were also recorded with application of K2SO4. Application of K fertilizers improved the availability of potassium in soil by 8.17% (254.2 kg ha−1). Potassium uptake in sugarcane crop ranged from 245 to 347 kg ha−1. The highest individual cane length (311 cm) was measured with application of K2SO4. Application of K2SO4 brings forth about 23.8% increments in millable canes, 14% in cane weight and 33.8% in cane yield over no K. Application of K also increased mean sucrose content in juice by 0.58 unit (17.0 pol per cent juice) over no use of K. Overall effect of K application on increasing sugarcane yield and sucrose content simultaneously brought forth improvement in commercial cane sugar (CCS) by 29.43% (10.50 t/ha) over no use of K. However, application of K2SO4 also improved CCS by 25.89% (11.67 t/ha) over the application of KCl

Changes in soil biological and biochemical characteristics in a long-term field trial on a sub-tropical inceptisol

Soil organic carbon (SOC), microbial biomass carbon (MBC), their ratio (MBC/SOC) which is also known as microbial quotient, soil respiration, dehydrogenase and phosphatase activities were evaluated in a long-term (31 years) field experiment involving fertility treatments (manure and inorganic fertilizers) and a maize (Zea mays L.)–wheat (Triticum aestivum L.)–cowpea (Vigna unguiculata L.) rotation at the Indian Agricultural Research Institute near New Delhi, India. Applying farmyard manure (FYM) plus NPK fertilizer significantly increased SOC (4.5–7.5 g kg-1), microbial biomass (124–291 mg kg-1) and microbial quotient from 2.88 to 3.87. Soil respiration, dehydrogenase and phosphatase activities were also increased by FYM applications. The MBC response to FYM+100% NPK compared to 100% NPK (193 vs. 291mg kg-1) was much greater than that for soil respiration (6.24 vs. 6.93 µlO2 g-1 h-1) indicating a considerable portion of MBC in FYM plots was inactive. Dehydrogenase activity increased slightly as NPK rates were increased from 50% to 100%, but excessive fertilization (150% NPK) decreased it. Acid phosphatase activity (31.1 vs. 51.8 µgPNP g-1 h-1) was much lower than alkali phosphatase activity (289 vs. 366 µgPNP g-1 h-1) in all treatments. Phosphatase activity was influenced more by season or crop (e.g. tilling wheat residue) than fertilizer treatment, although both MBC and phosphatase activity were increased with optimum or balanced fertilization. SOC, MBC, soil respiration and acid phosphatase activity in control (no NPK, no manure) treatment was lower than uncultivated reference soil, and soil respiration was limiting at N alone or NP alone treatments

Tracing source, distribution and health risk of potentially harmful elements (PHEs) in street dust of Durgapur, India

Street dust samples from Durgapur, the steel city of eastern India, were collected from five different land use patterns, i.e., national highways, urban residential area, sensitive area, industrial area and busy traffic zone during summer, monsoon, and winter to analyze the pollution characteristics,chemical fractionation, source apportionment and health risk of heavy metals (HMs). The samples were fractionated into ≤ 53 µm and analyzed for potentially harmful elements (PHEs) viz. Cd, Cr, Cu, Fe, Mn, Ni, Pb, and Zn. Summer season indicated higher concentrations of PHEs when compared to the other two seasons. Mean enrichment factor (EF), geo-accumulation index (Igeo), and contamination factor (CF) were high for Cd followed by Pb during all the three season in Durgapur. Chemical fractionation was executed in order to obtain distribution patterns of PHEs and to evaluate their bioavailable fractions in street dust samples. Mn was found to be highly bioavailable and bioavailability of the PHEs were in the order of Mn >Zn >Pb >Ni >Cd >Cu >Fe >Cr. Principal Component Analysis (PCA), cluster analysis, correlation analysis indicated the main sources of PHEs could be industrial, especially coal powered thermal plant, iron and steel industries and cement industries and vehicular. Multivariate analysis of variance (MANOVA) indicated that sites, seasons and their interaction were significantly affected by different PHEs as a whole. The health risk was calculated with total metal as well as mobile fraction of PHEs, which indicated that the actual non-carcinogenic risk due to bioavailable PHEs was less (HI<1) when compared to total concentrations of PHEs. Carcinogenic risk was observed for total Cr in street dust (Child: 4.6E-06; Adult: 3.6E-06)

Ameliorative effect of Lantana camara biochar on coal mine spoil and growth of maize (Zea mays)

The aftermath of surface mining is a wasteland deprived of vegetation, soil structure and biodiversity. The unearthed overburden material is nutrient deprived and can only support the growth of invasive weeds such as Lantana camera which often cause allelopathy. The aim of the study is to prepare biochar from these noxious weeds and use it as an amendment for the mine spoil reclamation. Lantana biochar (LB) was prepared and applied to mine spoil, and Zea mays L. growth on biochar amended mine spoil was monitored for three months. Biochar application in a coal mine spoil using LB is comparatively de novo approach for reclamation practitioners. LB was prepared at varying temperature (250, 350 and 450℃) and residence times (30, 45 and 60 min) and characterized. After characterization, the most recalcitrant biochar at 450℃ for 60 min was chosen for application for the study. A pot trial was conducted to study the effect of LB at 0, 5, 10, 20 and 30 g kg−1 dosage on the yield of Zea mays and mine spoil properties. Significant ameliorative effects were observed with increase in organic carbon content (2.9 times), cation exchange capacity (2 times), water holding capacity (0.13 times) and decrease in bulk density (0.5 times) in the mine spoil. The seedling vigour index and germination also increased significantly (p < .05) at 30 g kg−1 biochar treatment compared to control. The study concluded that LB has the potential to remediate coal mine spoils and promote re‐vegetation in degraded land

Biofilm development of Bacillus thuringiensis on MWCNT buckypaper: Adsorption-synergic biodegradation of phenanthrene

Adsorption-synergic biodegradation of a model PAH (phenanthrene, Phe) on MWCNT buckypaper surface with a potential PAH biodegrading bacterial strain Bacillus thuringiensis AT.ISM.11 has been studied in aqueous medium. Adsorption of Phe on buckypaper follows Dubinin-Ashtakhov model (R2 = 0.9895). MWCNT generally exerts toxicity to microbes but adsorbed layer of Phe prevents the direct contact between MWCNT and bacterial cell wall. FESEM study suggests that formation of biofilms occurred on buckypaper. Lower layer cells are disrupted and flattened as they are in direct contact with MWCNT but the upper layer cells of the developed biofilm are fully intact and functional. Force-distance curves of Bacillus thuringiensis AT.ISM.11 with buckypaper indicates adhesion forces varied from −10.3 to −15.6 nN with increasing contact time, which supports the phenomenon of biofilm formation. AFM surface statistical data of buckypaper suggests increase in bacterial cell count increases the Rms roughness (95.7242–632.565) while adhering to the buckypaper surface to form biofilm. We observed an enhanced Phe biodegradation of 93.81% from that of the 65.71% in 15 days’ study period, using buckypaper as a bio-carrier or a matrix for the microbial growth. GC-MS study identified phthalic acid ester as metabolite, which is the evidence of protocatechuate pathway degradation of Phe. Current study enlightens the interaction between hydrocarbons and microbes in presence of MWCNT buckypaper matrix in aqueous system for the first time. An enhancement in biodegradation of Phe by 28.10% has also been reported which can be a basis for CNT aided enhanced biodegradation studies in future

Effect of fly ash on carbon mineralization of biochar and organic manures added to mine spoil

Mine spoil is deprived of organic carbon. Reclamation and re-vegetation of mine spoil initiate photosynthetic carbon fxation and add soil organic matter. Fly ash (FA) generated from coal-fred power stations can be used for reclamation of mine spoil. Our research was aimed to assess the efect on FA on carbon mineralization of organic manures and biochar added to mine spoil. Incubation experiments were conducted to assess the carbon mineralization of farmyard manure (FYM), chicken litter (CL), plant litter (PL), and biochar (BC) added to mine spoil in the presence and absence of FA. After 1 year of incubation, the cumulative CO2 emission was lower for the FA-added mine spoil and it was higher for PL (4.42 vs. 5.09 g CO2–C/kg soil, with and without FA, respectively) and CL (3.75 vs. 4.07 g CO2–C/kg soil) followed by FYM (1.86 vs. 1.97) and BC (0.67 vs. 0.54 g CO2–C/kg soil). The labile C pool was signifcantly lower for FA-added mine spoil, whereas the stable carbon pool was higher in FA-added soils: FYM (1.4 vs. 0.6 g CO2–C/kg soil) and CL (2.20 vs. 3.17 g CO2–C/kg soil). Substrate-induced respiration, microbial biomass carbon (MBC), and dissolved organic carbon (DOC) were signifcantly higher under PL, CL, and FYM. FA increased MBC, but decreased DOC. Overall, FA decreased CO2 emission from mine spoil probably due to the interaction of organic matter with the surfaces on FA and the resultant physical protection against microbial decomposition