Evaluation of protected areas in Côte d’Ivoire and Ghana, West Africa, using a remote sensing-based approach

This study assesses the representation of defined ecoregions, slope profiles, and species richness of threatened mammals in the International Union for Conservation of Nature (IUCN)-listed protected areas in Ghana and Côte d’Ivoire. It also evaluates the exposure of protected area categories to the cumulative degree of human modification and their vulnerability to future agricultural expansion. Spatial gap and statistical analyses were performed using quantitative data from publicly available online global databases. Analyses indicated key conservation priorities for both countries: (1) to increase the protection of the Guinean forest–savanna mosaic, West Sudanian savanna, and Eastern Guinean forests, especially of the Eastern Guinean forests’ ecoregion associated with the Guinean forests of the West Africa biodiversity hotspot; (2) to increase the protected area coverage of flat lands and low slopes; and (3) to enhance the size and connectivity of existing protected areas, including restoring degraded habitats. The study emphasizes that improving the ability of tropical protected areas to conserve nature and mitigate anthropogenic threats should be a global conservation priority. Improving the data quality and detail within the World Database on Protected Areas and ground-truthing them are recommended urgently to support accurate and informative assessments

Choosing a medical specialty course in Italy: explorative analysis of factors related to the choice

Background: In order to be able to access a course of medical or surgical specialization, in addition to the degree and the qualification to the profession it is necessary to perform an entrance test. In this study we wanted to analyze the possible factors that determined the choice of a given graduate school and the place where to attend it in the year 2021.Study design: Cross-sectional study to evaluate the association between the type of graduate school, the score class obtained at the admission test, the location of the graduate school and the location of the degree. Methods: The evaluation of the association between site of specialty admission and degree, score at degree and score at admission test was performed by multiple correspondences analysis (MCA). Then, through a logistic regression model, the Odds Ratios (OR) and the respective confidence interval with 95% (95%CI) confidence level of the covariates on the probability of remaining in the same degree site, or in the same region or in the same geographical area, were estimated.Results The highest admission score and the highest age are significantly and independently associated with the probability of choosing, as a graduate school location, the same location where the degree was obtained.Conclusion: In conclusion, the choice of the course and the location of the specialty course is made in most cases, taking into account the score made in the exam, based on the location where you attended the course of study in medicine and surgery

SARS-CoV-2 strains bearing Omicron BA.1 spike replicate in C57BL/6 mice

Introduction: SARS-CoV-2, the cause of the COVID pandemic, is an RNA virus with a high propensity to mutate. Successive virus variants, including variants of concern (VOC), have emerged with increased transmission or immune escape. The original pandemic virus and early variants replicated poorly, if at all, in mice at least partly due to a mismatch between the receptor binding domain on the viral spike protein and the murine angiotensin converting enzyme 2 (ACE2). Omicron VOC emerged in late 2021 harboring > 50 new mutations, 35 of them in the spike protein. This variant resulted in a very large wave of infections, even in the face of prior immunity, albeit being inherently less severe than earlier variants. Reflecting the lower severity reported in humans, Omicron displayed attenuated infection in hamsters and also in the K18-hACE2 mouse model. K18-hACE2 mice express both the human ACE2 as well as the endogenous mouse ACE2. Methods: Here we infected hACE2knock-in mice that express only human ACE2 and no murine ACE2, or C57BL/6 wildtype mice with SARS-CoV-2 D614G (first-wave isolate), Delta or Omicron BA.1 variants and assessed infectivity and downstream innate immune responses. Results: While replication of SARS-CoV-2 Omicron was lower in the lungs of hACE2knock-in mice compared with SARS-CoV-2 D614G and VOC Delta, it replicated more efficiently than the earlier variants in C57BL/6 wildtype mice. This opens the opportunity to test the effect of host genetics on SARS-CoV-2 infections in wildtype mice. As a proof of principle, we tested Omicron infection in mice lacking expression of the interferon-alpha receptor-1 (IFNAR1). In these mice we found that loss of type I IFN receptor signaling resulted in higher viral loads in the lungs were detected. Finally, using a chimeric virus of first wave SARS-CoV-2 harboring the Omicron spike protein, we show that Omicron spike increase infection of C57BL/6 wildtype mice, but non-spike genes of Omicron confer attenuation of viral replication. Discussion: Since this chimeric virus efficiently infected C57BL/6 wildtype mice, and replicated in their lungs, our findings illustrate a pathway for genetic mapping of virushost interactions during SARS-CoV-2 infection

ISARIC-COVID-19 dataset: A Prospective, Standardized, Global Dataset of Patients Hospitalized with COVID-19

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Neurological manifestations of COVID-19 in adults and children

Different neurological manifestations of coronavirus disease 2019 (COVID-19) in adults and children and their impact have not been well characterized. We aimed to determine the prevalence of neurological manifestations and in-hospital complications among hospitalized COVID-19 patients and ascertain differences between adults and children. We conducted a prospective multicentre observational study using the International Severe Acute Respiratory and emerging Infection Consortium (ISARIC) cohort across 1507 sites worldwide from 30 January 2020 to 25 May 2021. Analyses of neurological manifestations and neurological complications considered unadjusted prevalence estimates for predefined patient subgroups, and adjusted estimates as a function of patient age and time of hospitalization using generalized linear models. Overall, 161 239 patients (158 267 adults; 2972 children) hospitalized with COVID-19 and assessed for neurological manifestations and complications were included. In adults and children, the most frequent neurological manifestations at admission were fatigue (adults: 37.4%; children: 20.4%), altered consciousness (20.9%; 6.8%), myalgia (16.9%; 7.6%), dysgeusia (7.4%; 1.9%), anosmia (6.0%; 2.2%) and seizure (1.1%; 5.2%). In adults, the most frequent in-hospital neurological complications were stroke (1.5%), seizure (1%) and CNS infection (0.2%). Each occurred more frequently in intensive care unit (ICU) than in non-ICU patients. In children, seizure was the only neurological complication to occur more frequently in ICU versus non-ICU (7.1% versus 2.3%, P < 0.001). Stroke prevalence increased with increasing age, while CNS infection and seizure steadily decreased with age. There was a dramatic decrease in stroke over time during the pandemic. Hypertension, chronic neurological disease and the use of extracorporeal membrane oxygenation were associated with increased risk of stroke. Altered consciousness was associated with CNS infection, seizure and stroke. All in-hospital neurological complications were associated with increased odds of death. The likelihood of death rose with increasing age, especially after 25 years of age. In conclusion, adults and children have different neurological manifestations and in-hospital complications associated with COVID-19. Stroke risk increased with increasing age, while CNS infection and seizure risk decreased with age

The Gender Impact Assessment among Healthcare Workers in the SARS-CoV-2 Vaccination—An Analysis of Serological Response and Side Effects

Healthcare professionals are considered to be at high risk of exposure and spread of SARS-CoV-2, and have therefore been considered a priority group in COVID-19 vaccination campaign strategies. However, it must be assumed that the immune response is influenced by numerous factors, including sex and gender. The analysis of these factors is an impact element for stratifying the population and targeting the vaccination strategy. Therefore, a large cohort of healthcare workers participating in the Italian vaccination campaign against SARS-CoV-2 has been studied to establish the impact of sex and gender on vaccination coverage using the Gender Impact Assessment approach. This study shows a significant difference in the antibody titers among different age and sex groups, with a clear decreasing trend in antibody titers in the older age groups. Overall, the serological values were significantly higher in females; the reported side effects are more frequent in females than in males. Therefore, disaggregated data point out how the evaluation of gender factors could be essential in COVID-19 vaccination strategies. On this biomedical and social basis, suggestions are provided to improve the SARS-CoV-2 vaccination campaign in healthcare professionals. Still, they could be adapted to other categories and contexts.</jats:p

Do local curriculum scores correlate with national residency test results? A pluriannual, nationwide survey of Italian Medical Universities

In Italy, to match medical doctors to specialty residencies, the Minister of Education organizes a national multiple choice questions exam composed of 140 items. The curriculum grants additional points based on the candidates’ grade average and graduation mark. Both national and international policies assume that curriculum scores can be evaluated in absolute terms, irrespective of the course attended by the candidate or its University of graduation. A fair inquiry over the current criteria of selection is not available to this day. Hence, this study aimed at investigating the degree of association between results in the Italian national residency test and the score given for each candidate’s curriculum. We calculated Spearman’s correlation coefficients between the score obtained at the 2019 test and curriculum scores. A moderate/high degree of correlation (rho=0.600) was reached. Spearman’s rho ranged from 0.447 to 0.788, considering the single Universities. We thus measured a modest but non-negligible between-University variability. As differences between test and curriculum scores across institutions may determine the admission or not to the residency for many candidates, the authors call for broader attention on the topic.</jats:p