Comparison of VP7 antigenic epitope sites between Pakistani G3 strains and rotavirus vaccine Rotarix^TM and RotaTeq^TM.

<p><b>(A)</b> Antigenic residues are divided into three antigenic epitopes 7-1a, 7-1b and 7–2. Amino acid highlighted in green are those that differ from G3 strain of RotaTeq^TM while those in gray are different from Rotarix^TM. <b>(B)</b> Surface representation of VP7 trimer (PDB 3FMG). Antigenic epitopes are colored in red 7-1a, purple 7-1b and green 7–2. Surface exposed residues that differ between Pakistani G3 and vaccine strains of Rotarix^TM and RotaTeq^TM are shown in cyan.</p

Alignment of antigenic residues in VP4 between the strains contained in Rotarix^TM and RotaTeq^TM and Pakistani P[8] and P[4].

<p><b>(A)</b> Antigenic residues are divided in three antigenic epitopes in VP8* (8–1, 8–2, 8–3 and 8–4). Amino acids in green are different from both Rotarix^TM and G3 strain of RotaTeq^TM. <b>(B)</b> Surface representation of the VP8* core (PDB 1KQR). Antigenic epitopes are colored red 8–1, orange 8–2, green 8–3 and blue 8–4. Surface exposed residues that differ between Pakistani G3P[8] and vaccine Rotarix^TM and RotaTeq^TM are shown in cyan.</p

Phylogenetic analysis of VP7 gene segment of G3 rotavirus strains.

<p>Phylogenetic tree was reconstructed using neighbor-joining method. Bootstrap values were calculated using 1000 replicates. Bootstrap values less than 60 are not shown. Filled triangles represent G3 strains detected in this study.</p

Association of clinical infection in newborns compared to the mother’s gestational stage of CMV exposure.

<p>Association of clinical infection in newborns compared to the mother’s gestational stage of CMV exposure.</p

Oligonucleotide primers and thermal conditions used for PCR and sequencing of cytomegalovirus.

<p>Oligonucleotide primers and thermal conditions used for PCR and sequencing of cytomegalovirus.</p

Clinical Abnormalities found in Newborns with Symptomatic Congenital CMV Infection.

<p>Clinical Abnormalities found in Newborns with Symptomatic Congenital CMV Infection.</p

Genotype distribution of CMV in neonates born with congenital infection.

<p>Genotype distribution of CMV in neonates born with congenital infection.</p

Phylogenetic analysis of gB, gN and gH genotypes of HCMV.

<p>The reference strains and isolates detected through BLAST are given for genetic comparison. The phylogenetic tree with 1000 bootstrap replicates was reconstructed using neighbor joining method and Kimura two-parameter distances model incorporated in MEGA v5.0. The number at the nodes indicates bootstrap values shown above 50. Fig 2A, 2B and 2C represent genetic relationships of gB, gH and gN genotypes of CMV respectively. The strains detected in this study are represented by sample identification with codes mentioned as PAK: Pakistan, NIH: National Institute of Health Islamabad.</p

Logistic regression analysis of demographic and clinical features of mothers infected with active CMV infection.

<p>Logistic regression analysis of demographic and clinical features of mothers infected with active CMV infection.</p

Results of IgM, IgG and PCR testing for diagnosis of CMV infection in pregnant women.

<p>Results of IgM, IgG and PCR testing for diagnosis of CMV infection in pregnant women.</p