EFFECTS OF SONICATION ON SIZE DISTRIBUTION AND ENTRAPMENT OF LYNESTRENOL TRANSFEROSOME

Objective: The aim of this study was to develop transferosome vesicles for the transdermal drug delivery of lynestrenol.Methods: The lynestrenol transferosome vesicle was made by encapsulating the drug in a variation of phosphatidylcholine and Tween 80 by the thinlayerhydration method. The resulting transferosome vesicles were modified with a time variation of 30, 60, 90, and 120 min, and sonication variationswere paused and not paused. Particle size evaluation, polydispersity (PDI), and entrapment efficiency (%EE) were carried out on the variation ofsonication time.Results: The evaluation results showed that sonication without pauses showed better %EE and particle size than sonication with pauses andincreasing concentration of Tween 80 (edge activator). The %EE increased, and particle size decreased with increasing sonication time; PDI of vesicleswas heterogeneous with increasing sonication time. The %EE in formulas F1 and F2 after 120 min was 73.06% and 76.06% (paused) and 80.40% and82.97% (without paused). The particle size of formula F1 and F2 after 120 min 575.4 nm and 471.6 nm (paused) and 524.1 nm and 434.7 nm (withoutpaused). The PDI formulas of F1 and F2 after 120 min were 0.69 and 0.763 (paused) and 0.84 and 0.59 (without paused).Conclusion: Based on the results of the transferosome vesicle characteristics, it was shown that the optimal vesicle composition for packaginglynestrenol was vesicles that were composed of phosphatidylcholine and Tween 80 without pauses and could potentially be used as a transdermaldrug delivery system

IMPROVING TRANSDERMAL DRUG DELIVERY SYSTEM FOR MEDROXYPROGESTERONE ACETATE BY OLIVE OIL AND DIMETHYLSULFOXIDE (DMSO) AS PENETRATION ENHANCERS: IN VITRO PENETRATION STUDY

Objective: Medroxyprogesterone Acetate (MPA) using a transdermal drug delivery system for contraception by passive diffusion is limited by the skin barrier properties. Penetration enhancers such as olive oil (fatty acid permeation enhancer) and DMSO (chemical enhancer) can be used. The objective of this study was to overcome MPA penetration problem by using olive oil and DMSO. Methods: An in vitro penetration study using the Franz diffusion cells was performed. The first penetration study used MPA in olive oil (O) and MPA in coconut oil (C) with the concentration 100 μg/ml to each sample and MPA suspension as a control with the same concentration. The second study used MPA in olive oil with the concentration 200.0 μg/ml (A), MPA in olive oil with 0.5% DMSO with the concentration 200.0 μg/ml (B), and MPA in olive oil with 1% DMSO with the concentration 200 μg/ml (C). Results: MPA penetration test for olive oil+0.5% DMSO had flux value 4.24±0.074 μg/cm2. hr and it was not significantly different (t-test, P>0.05) with olive oil+1% DMSO. While the MPA penetration test in only Olive oil had flux value 0.90±0.0087 μg/cm2. hr. Conclusion: This research concluded that olive oil and 0.5% DMSO could improve the penetration of MPA into skin membrane by 4.5 times more than olive oil alone

FORMULASI, EVALUASI DAN UJI STABILITAS FISIK SEDIAAN GEL ANTIOKSIDAN EKSTRAK TALI PUTRI (Cassytha filiformis L)

Tali putri (Cassytha filiformis L) termasuk ke dalam famili lauraceae, yang memiliki aktivitas antioksidan alami. Tanaman ini banyak dijumpai di kawasan pesisir Pantai Panjang Kota Bengkulu. Tujuan penelitiaan ini adalah mendapatkan formula gel antioksidan ekstrak Tali Putri (Cassytha filiformis L) terbaik berdasarkan evaluasi dan uji stabilitasnya. Tumbuahan Tali Putri (Cassytha filiformis L) diekstrak dengan metode maserasi selama 3x24 jam menggunakan pelarut metanol. Penelitian ini menggunakan ekstrak tali putri (Cassytha filiformis L) sebanyak 3%, yang diformulasikan ke dalam 3 formula dengan variasi basis HPMC, yaitu 7% (F1), 10% (F2), dan 15% (F3). Uji evaluasi yang dilakukan yaitu uji homogenitas, uji organoleptis, uji pH, dan uji viskositas. Uji stabilitas fisik menggunakan metode cycling test dilakukan selama 3 hari. Hasil pengujian randemen ekstrak yang diperoleh adalah 2,812%. Uji pH menunjukan gel memiliki pH 6,4 (F1), 6,3 (F2), dan 6,2 (F3). Uji viskositas menunjukan gel memiliki viskositas 2530 cps (F1), 1610 cps (F2), 1920 cps (F3). Uji stabilitas metode cycling test terjadi sineresis di setiap formulasi dengan waktu terlama pada F1. Gel mengandung ekstrak tali putri (Cassytha filiformis L) yang terbaik berdasarkan hasil evaluasi dan uji stabilitas adalah formula 1 (F1)

EDUKASI PEMBUATAN JAMU YANG BAIK DAN AMAN PADA KELOMPOK JAMU GENDONG DI KOTA BENGKULU

Usaha Jamu Gendong (UJG) is a herbal medicine business carried out by individuals using traditional medicine ingredients in the form of fresh liquid to be sold directly to consumers. This service activity was aimed to increasing knowledge and providing skills on how to make good and safe jamu for UJG group in Bengkulu City. This activity was carried out on October 22, 2022 with 20 participants. This service activity is carried out using discussion and demonstration methods. Evaluation of activities is carried out by giving questionnaire sheets given before and after the activity. The results of the questionnaire evaluation obtained that the average score of 20 participants before and after the activity was 90.6 and 99.2, respectively. From the implementation of the activities, it can be concluded that there is an increase in the knowledge of the herbal medicine group regarding how to make good and safe traditional medicines