Birefringence and transmission of an antireflection-coated sulfur-free cadmium selenide Wollaston prism at 30 K

We present a determination of the change with temperature and wavelength of the degree of birefringence of a cold (similar to 30K) Wollaston prism constructed from antireflection (AR)-coated sulfur-free cadmium selenide (CdSe). We compare the normalized birefringence for the material to that estimated by the Sellmeier-4 formula and to previously published measurements of a warm sample of sulfur-free CdSe. Finally, we measure the transmission as a function of wavelength

The microbial biodiversity at the archeological site of Tel Megiddo (Israel)

IntroductionThe ancient city of Tel Megiddo in the Jezreel Valley (Israel), which lasted from the Neolithic to the Iron Age, has been continuously excavated since 1903 and is now recognized as a World Heritage Site. The site features multiple ruins in various areas, including temples and stables, alongside modern constructions, and public access is allowed in designated areas. The site has been studied extensively since the last century; however, its microbiome has never been studied. We carried out the first survey of the microbiomes in Tel Megiddo. Our objectives were to study (i) the unique microbial community structure of the site, (ii) the variation in the microbial communities across areas, (iii) the similarity of the microbiomes to urban and archeological microbes, (iv) the presence and abundance of potential bio-corroding microbes, and (v) the presence and abundance of potentially pathogenic microbes.MethodsWe collected 40 swab samples from ten major areas and identified microbial taxa using next-generation sequencing of microbial genomes. These genomes were annotated and classified taxonomically and pathogenetically.ResultsWe found that eight phyla, six of which exist in all ten areas, dominated the site (>99%). The relative sequence abundance of taxa varied between the ruins and the sampled materials and was assessed using all metagenomic reads mapping to a respective taxon. The site hosted unique taxa characteristic of the built environment and exhibited high similarity to the microbiome of other monuments. We identified acid-producing bacteria that may pose a risk to the site through biocorrosion and staining and thus pose a danger to the site’s preservation. Differences in the microbiomes of the publicly accessible or inaccessible areas were insignificant; however, pathogens were more abundant in the former.DiscussionWe found that Tel Megiddo combines microbiomes of arid regions and monuments with human pathogens. The findings shed light on the microbial community structures and have relevance for bio-conservation efforts and visitor health

Generation of ultrabright tunable polarization entanglement without spatial, spectral, or temporal constraints

The need for spatial and spectral filtering in the generation of polarization entanglement is eliminated by combining two coherently-driven type-II spontaneous parametric downconverters. The resulting ultrabright source emits photon pairs that are polarization entangled over the entire spatial cone and spectrum of emission. We detect a flux of $\sim$12 000 polarization-entangled pairs/s per mW of pump power at 90% quantum-interference visibility, and the source can be temperature tuned for 5 nm around frequency degeneracy. The output state is actively controlled by precisely adjusting the relative phase of the two coherent pumps.Comment: 10 pages, 5 figure

genomation: a toolkit to summarize, annotate and visualize genomic intervals

Summary: Biological insights can be obtained through computational integration of genomics data sets consisting of diverse types of information. The integration is often hampered by a large variety of existing file formats, often containing similar information, and the necessity to use complicated tools to achieve the desired results. We have built an R package, genomation, to expedite the extraction of biological information from high throughput data. The package works with a variety of genomic interval file types and enables easy summarization and annotation of high throughput data sets with given genomic annotations. Availability and implementation: The software is currently distributed under MIT artistic license and freely available at http://bioinformatics.mdc-berlin.de/genomation, and through the Bioconductor framework. Contact: [email protected], [email protected], [email protected], or [email protected]

Punishment Feedback Impairs Memory and Changes Cortical Feedback-Related Potentials During Motor Learning

© Copyright © 2020 Hill, Stringer, Waddell and Del Arco. Reward and punishment have demonstrated dissociable effects on motor learning and memory, which suggests that these reinforcers are differently processed by the brain. To test this possibility, we use electroencephalography to record cortical neural activity after the presentation of reward and punishment feedback during a visuomotor rotation task. Participants were randomly placed into Reward, Punishment, or Control groups and performed the task under different conditions to assess the adaptation (learning) and retention (memory) of the motor task. These conditions featured an incongruent position between the cursor and the target, with the cursor trajectory, rotated 30° counterclockwise, requiring the participant to adapt their movement to hit the target. Feedback based on error magnitude was provided during the Adaptation condition in the form of a positive number (Reward) or negative number (Punishment), each representing a monetary gain or loss, respectively. No reinforcement or visual feedback was provided during the No Vision condition (retention). Performance error and event-related potentials (ERPs) time-locked to feedback presentation were calculated for each participant during both conditions. Punishment feedback reduced performance error and promoted faster learning during the Adaptation condition. In contrast, punishment feedback increased performance error during the No Vision condition compared to Control and Reward groups, which suggests a diminished motor memory. Moreover, the Punishment group showed a significant decrease in the amplitude of ERPs during the No Vision condition compared to the Adaptation condition. The amplitude of ERPs did not change in the other two groups. These results suggest that punishment feedback impairs motor retention by altering the neural processing involved in memory encoding. This study provides a neurophysiological underpinning for the dissociative effects of punishment feedback on motor learning

The Fe XXII I(11.92 A)/I(11.77 A) Density Diagnostic Applied to the Chandra High Energy Transmission Grating Spectrum of EX Hydrae

Using the Livermore X-ray Spectral Synthesizer, which calculates spectral models of highly charged ions based primarily on HULLAC atomic data, we investigate the temperature, density, and photoexcitation dependence of the I(11.92 A)/I(11.77 A) line ratio of Fe XXII. We find that this line ratio has a critical density n_c \approx 5x10^13 cm^-3, is approximately 0.3 at low densities and 1.5 at high densities, and is very insensitive to temperature and photoexcitation, so is a useful density diagnostic for sources like magnetic cataclysmic variables in which the plasma densities are high and the efficacy of the He-like ion density diagnostic is compromised by the presence of a bright ultraviolet continuum. Applying this diagnostic to the Chandra High Energy Transmission Grating spectrum of the intermediate polar EX Hya, we find that the electron density of its T_e \approx 12 MK plasma is n_e = 1.0^{+2.0}_{-0.5} x 10^14 cm^-3, orders of magnitude greater than that typically observed in the Sun or other late-type stars.Comment: 11 pages including 3 encapsulated postscript figures; LaTeX format, uses aastex.cls; accepted on 2003 April 3 for publication in The Astrophysical Journa

Arginine side chain interactions and the role of arginine as a gating charge carrier in voltage sensitive ion channels

Gating charges in voltage-sensing domains (VSD) of voltage-sensitive ion channels and enzymes are carried on arginine side chains rather than lysine. This arginine preference may result from the unique hydration properties of the side chain guanidinium group which facilitates its movement through a hydrophobic plug that seals the center of the VSD, as suggested by molecular dynamics simulations. To test for side chain interactions implicit in this model we inspected interactions of the side chains of arginine and lysine with each of the 19 non-glycine amino acids in proteins in the protein data bank. The arginine guanidinium interacts with non-polar aromatic and aliphatic side chains above and below the guanidinium plane while hydrogen bonding with polar side chains is restricted to in-plane positions. In contrast, non-polar side chains interact largely with the aliphatic part of the lysine side chain. The hydration properties of arginine and lysine are strongly reflected in their respective interactions with non-polar and polar side chains as observed in protein structures and in molecular dynamics simulations, and likely underlie the preference for arginine as a mobile charge carrier in VSD