NIRS discrimination of broiler rabbits fed with increasing levels of false flax (Camelina sativa L.) seeds in relationship to the fatty acid profiles

Three groups of ten young rabbits each received an enriched diet with false flax (Camelina sativa L.) seeds at 0% (C), 10% (M), and 15% (H), respectively. At the end of the experimental period, which lasted 50 days, all the rabbits were slaughtered. The longissimus dorsi (LD) muscle and perirenal fat samples were collected at 24 h post mortem from each carcass and analysed with a GC method for the fatty acid (FA) profiles and their indexes. Spectroscopy was conducted using a Model LSP LabSpec-Pro portable UV-Vis.NIR spectrophotometer (350-2500nm). The perirenal fat was directly examined. The samples derived from thawed LD muscles (2 cm ø x 2 cm long.) were previously fixed in 95% ethanol, stored for 3 days and finally scanned after 2 and 24 hours air exposition of the tissues. Discrimination of individuals between couple of groups, fitted 1 or 2 dummy values, was performed by a Modified Partial Least Square Discriminant Analysis by the WinISI II software (Infrasoft International, Port Matilda, PA, USA) allowing one passage for the removal of the outliers. The cross-validated 1-VR (Variance Ratio) coefficient was retained for comparative purposes.................

Raw and extruded pea (Pisum sativum) and lupin (Lupinus albus var. Multitalia) seeds as protein sources in weaned piglets' diets: effect on growth rate and blood parameters

The 42 days trial was carried out using 140 piglets weaned at 28 days of age. The piglets were allocated according to weight and sex to the 5 dietary treatments with 7 replicates for each treatments (4 pens x 4 castrated males and 3 pens x 4 females). The piglets were fed according to the following experimental design: 1) control diet (CTR) with soybean meal (SBM) 44% c.p. as protein source; 2) CRT diets with 200 g/kg of raw pea (Pisum sativum) (RP); 3) CTR diet with 200 g/kg extruded pea (EP); 4) CRT diet with 170 g/kg raw lupin (Lupinus albusvar. Multitalia) (RL); 5) CTR diet with 170 g/kg of extruded lupin (EL). During the trial, animals were weighed at 0 - 21 and 42 days from the start of the trial. Feed intake was monitored and feed conversion ratio was calculated for the periods 0-21 d and 22-42 d. At the end of the trial, blood samples were taken for 14 animals for each dietary treatment (2 animals per replicate) and analysed for total protein, urea and liver activity (ALT, AST and ALP parameters). Average daily weight gain and feed intake did not differ according to dietary treatments whereas during the total experimental period (0-42 d), feed conversion ratio was higher for EP vsCTR diet (2.35 vs2.09, respectively; P <0.05). The growth rate for diets with extruded protein sources compared with diets containing the raw ingredients did not differ. Feed conversion ratio for the RP was numerically high- er than for the EP (2.35 vs2.16 and 2.76 vs2.32, respectively during 22-42 d and 0-42 d periods). Blood parameters did not show significant difference among dietary treatments except for higher total protein for CTR diet vsRL diet, EL and RP (67.3 vs62.2, 62.8 and 63.6 g/l, respectively; P<0.05) and urea that resulted the highest with CTR diet vsRL and RL (4.7 vs3.7 and 3.8 mmol/l respectively; P<0.05)

Pea seeds (Pisum sativum), faba beans (Vicia faba var. minor) and lupin seeds (Lupinus albus var. multitalia) as protein sources in broiler diets: effect of extrusion on growth performance

The effect of extrusion of pea seeds (Pisum sativum) (PS), faba bean (Vicia faba, variety minor) (FB) and lupin seeds (Lupinus albus, variety multitalia) (LS) on broiler performance were evaluated. Four hundred sixty two 1d-old Ross male chicks, Marek vaccinated, were randomly assigned to seven dietary treatments (3 pens per treatment/22 birds per pen). Chicks were floor housed, ad libitum fed isocaloric and isonitrogenous diets and had free access to water. Artificial light was provided 10 h/d. The bulk of the base diet (control diet) was corn (48.8%, 53.7% and 57%), solvent-extracted soy- bean meal (42.8%, 37.3% and 33.4%), corn oil (4.4%, 5.2% and 6.3%), plus synthetic amino acids, minerals, trace minerals and vitamins, respectively for the 1-10d-old, 11-28d-old and 29 to 42d-old growing periods. The amounts of PS, FB and LS used on an as fed basis were: PS and extruded PS (EPS): 353 (1-10d-old), 356 (11-28d-old) and 350 (29- 42d-old) g/kg; FB and extruded FB (EFB): 479 (1-10d-old), 497 (11-28d-old) and 500 (29-42d old) g/kg; LS and extrud- ed LS (ELS): 360 (1-10d-old) and 300 (11-42d-old) g/kg. High levels of pea (350 g/kg) and faba bean (500 g/kg) did not show negative effects on body weight gain (BWG) and bird feed intake compared to control. Lupin at the 300 g/kg level reduced (P< 0.05) the BWG during the finishing period (22 to 42 d), however the effect disappeared over the whole experimental period (1-42 d) compared to the control group. The ELS group had a lower (P< 0.01) feed intake com- pared to the control group and to the LS group. The feed conversion rate (FCR) was similar among groups for the whole experimental period; however during the grower period the FCR was higher (P< 0.05) for the PS, FB and EFB groups com- pared to the control group. Birds consuming the PS diet had a reduced (P< 0.05) eviscerated carcass yield compared to the control group. The breast meat percent yield was higher (P< 0.01) for birds consuming the FB and EFB diets compared to the control group. There were no statistical differences in percent yield of the leg quarters and in blood parameters

Raw Pea (Pisum sativum), raw Faba bean (Vicia faba var. minor) and raw Lupin (Lupinus albus var. multitalia) as alternative protein sources in broiler diets

The ban of the meat and bone meal for entering animal diets and the concern of transgenic feeds poses a challenge toanimal nutritionists in Europe. The challenge is to find homegrown protein-rich feedstuffs, making sure no antinutritionalfactors are present which could interfere in the animals’ performance. The raw Pea (Pisum sativum) (RP), raw Fababean (Vicia faba, variety minor) (RFb) and raw Lupin (Lupinus albus, variety multitalia) (RL) were evaluated as alternativeprotein sources into broiler diets. Six hundred thirty 1d-old Ross male chicks, Marek vaccinated, were randomlyassigned to seven dietary treatments (5 pens per treatment/18 birds per pen). Chicks were floor housed, ad libitum fedisocaloric and isonitrogenous diets and had free access to water. Artificial light was provided 10 h/d. The bulk of the basediet (control diet) was corn (48.7%, 56.6% and 57%), solvent-extracted soybean meal (42.8%, 37.3% and 33.4%), cornoil (4.4%, 5.2% and 6.3%), plus synthetic amino acids, minerals, trace minerals and vitamins, respectively for the 1-10d-old, 11-28d-old and 29 to 42d-old growing periods. The RP, RFb and RL entered diets in substitution of the soybeanand corn according to the cost optimization (P100, Fb100 and L100, respectively for RP, RFb and RL) and at half of theoptimized quantity (RP50, RFb50 and RL50, respectively for RP, RFb and RL). The amount used as fed basis for the higherlevel of inclusion were: P100: 350 g/kg for all diets; Fb100: 480 g/kg (1-10d-old) and 500 g/kg (11-42d-old); L100:360 g/kg (1-10d-old) and 300 g/kg (11-42d-old). The average daily gain (ADG) were lower (P < 0.05) in the RP groupcompared to the control group. Over the whole period of growth, the RFb group had similar ADG compared to the controlgroup and for both levels of inclusion, whereas reduced (P < 0.05) ADG were observed in the RL100 group. Reduced(P < 0.05) ADG were also observed for the RFb100 and the RL100 groups when calculated over the first three weeks ofgrowth. Birds performance was improved (P < 0.05) in the RL50 group. No effects were observed on dressing percentageand breast and leg quarter cuts. The RFb and RL could represent valuable protein feeds in broilers diet formulation