35 research outputs found

    Prevalence of occult hepatitis B infection among blood donors in Saint Petersburg

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    The aim of this study was to assess the prevalence of occult hepatitis B infection among blood donors in St. Petersburg, as well as to characterize the identified virus isolates. The study material was represented by 2800 blood plasma samples collected in 2019 from blood donors living in St. Petersburg. The ELISA study for HBV marker rate consisted of HBsAg, anti-HBs IgG, anti-HBcore IgG. HBV DNA was analyzed by nested PCR with real-time hybridization-fluorescence detection on three targets allowing to determine virus DNA at low viral load, including HBsAg-negative chronic hepatitis B. Hepatitis B serological markers were detected in 69.43% of those surveyed, HBsAg was found in 0.43% of individuals, and all of which donated blood first time. A significant excess of the anti-HBcore IgG antibodies occurrence among primary donors (15.1%) compared with repeated/regular donors (7.48%) was shown. The prevalence of virus DNA in the group was 3.14%, including 2.71% of cases in HBsAg-negative CHB. Based on phylogenetic analysis of 88 isolates, HBV subgenotypes were determined in the following order: D1 and D2, 40.91% each, D3 and A2, 9.09% each. While determining the serological subtype in detected isolates, the serotype ayw3 (52.27%) vs ayw2 (46.59%) and adw2 (10.23%) prevailed. Drug resistance mutations, including compensatory ones, were detected in six examined patients (6.82%). In all genotype D isolates, multiple amino acid substitutions were identified in the RT, SHB, MHB, LHB, and Core regions; mutations in the preCore region were detected in 21.59% samples. In the MHR of the HBV genotype D genome, twenty-six positions were identified in which amino acid substitutions occurred, and all isolates showed modifications at positions 113, 114, 131, 134, 159, 161, 168, in 76 — at position 122, in 68 — at position 127, in 36 — at position 118, in 24 — at position 128. In HBV A2 isolates, mutations T113S, S143T, Y161F were identified. Nine isolates in the preCore region showed a polymorphism including a stop codon W28*W; in five isolates the W28S substitution was shown in the same position, and the W28*S variant was found in one more sample. The high incidence of HBsAg-negative CHB cases among blood donors, as well as the predominance of HBV isolates that simultaneously carry mutations resulting in diagnostic failure of HBsAg tests and prophylactic failure of immunoglobulin or vaccines and virus reactivation, mutations that contribute to disease progression obviously pose a threat to health and require to be further examined

    Circularly polarized electroluminescence from silicon nanostructures heavily doped with boron

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    The circularly polarized electroluminescence (CPEL) from silicon nanostructures which are the p-type ultra-narrow silicon quantum well (Si-QW) confined by {\delta}-barriers heavily doped with boron, 5 10^21 cm^-3, is under study as a function of temperature and excitation levels. The CPEL dependences on the forward current and temperature show the circularly polarized light emission which appears to be caused by the exciton recombination through the negative-U dipole boron centers at the Si-QW {\delta}-barriers interface

    Influence of Structure of Detrital Food Webs on Fusarium Head Blight of Winter Wheat

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    Conventional methods for Fusarium head blight (FHB) control are ineffective. A better understanding of the mechanisms linking the abundance of Fusarium species in soil before winter wheat flowering and mycotoxin content in mature grain may help to improve the effectiveness of methods for FHB control. In this study, we established a field experiment aimed to trace the impact of three types of organic mulch with different C:N ratios on the structure of detrital food webs and the manifestation of winter wheat FHB. T2-toxin content in grain was significantly higher in N-poor treatment (52.1 ± 0.2 µg g−1) compared to N-rich treatment (40.4 ± 1.6 µg g−1). The structure of detrital food webs in the studied treatments changed significantly after mulch addition; the abundance of soil saprophages and mycophages increased up to 50%. Based on the results of mixed-effects modeling, the abundance of herpetobionts and soil mesofauna were positively associated with an increase in Fusarium biomass in grain. The increase in the content of T2-toxin in the grain was associated with an increase in the abundance of earthworms and phytophagous macrofauna in the soil. Results suggest the existence of a previously undescribed mechanism for FHB infection by transfer of pathogenic spores by soil invertebrates, while the content of mycotoxins in grain can be triggered by the grazing activity of soil phytophagous invertebrates

    High-temperature quantum kinetic effect in silicon nanosandwiches

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    The negative- U impurity stripes confining the edge channels of semiconductor quantum wells are shown to allow the effective cooling inside in the process of the spin-dependent transport, with the reduction of the electron-electron interaction. The aforesaid promotes also the creation of composite bosons and fermions by the capture of single magnetic flux quanta on the edge channels under the conditions of low sheet density of carriers, thus opening new opportunities for the registration of the quantum kinetic phenomena in weak magnetic fields at high-temperatures up to the room temperature. As a certain version noted above we present the first findings of the high temperature de Haas-van Alphen, 300 K, quantum Hall, 77 K, effects as well as quantum conductance staircase in the silicon sandwich structure that represents the ultra-narrow, 2 nm, p-type quantum well (Si-QW) confined by the delta barriers heavily doped with boron on the n-type Si (100) surface
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