Schizophyllum Commune As Nano-Factory For Biosynthesis Of Silver Nanoparticles

Silver nanoparticles (AgNPs), are the noble metal nanoparticles that are being studied extensively due to their antimicrobial properties[1]

Production Of Citronellol As An Artificial Flavour Using Whole Cell Saccharomyces Cerevisiae : Design Of A Continuous Closed-Gas-Loop Bioreactor For Biotransformation (CCGLBB).

The production of artificial flavors with highly chemo-, regio and stereo specific compounds has become an important requirement in food industry. Production of artificial flavors by chemical catalysis has been used previously in converting a reactant into a useful product as a substitute to the natural sources. Penghasilan perasa timan yang mempunyai sifat kemo, regio dan stereo spesifik yang tinggi telah menjadi keutamaan dalam industri makanan kini. Penghasilan perasa tiruan melalui kaedah kimia telah lama digunakan dalam menukarkan reaktan kepada produk yang berguna sebagai satu alternatif kepada pengekstrakan daripada sumber asal

In vitro antifungal activities and phytochemical analysis of filamentous white-rot fungi, schizophyllum commune

In this study, the in-vitro antifungal activity and phytochemical analysis of Schizophyllum commune extracts have been investigated. The antifungal activity was tested against 11 species of selected wood degrading fungi of rubberwood. The results showed that water, methanol and ethanol extracts significantly inhibited the growth of wood degrading fungi with minimum inhibitory concentration (MIC) ranges 0.16-5.00 μg/μL. P. sanguineus was found as the strongest wood degrading fungus where it required the highest concentration of S. commune crude extracts (≥ 5.00 μg/μL) to inhibit its mycelia growth. Phytochemicals analysis revealed that the extracts contained flavonoid, phenol and saponin. The methanol extracts of S. commune was then applied on the rubberwood blocks and found that the growth of P. sanguineus was inhibited effectively at 5.00 μg/μL

Production of biomass by Schizophyllum commune and its antifungal activity towards rubberwood-degrading fungi

Rubberwood is the most popular timber for furniture manufacturing industry in Malaysia. Major drawback concerned that rubberwood is very prone to attack by fungi and wood borers, and the preservation method using boron compounds exhibited hazardous effect to the workers. Fungal-based biological control agents have gained wide acceptance and Schizophyllum commune secondary metabolite played an important role in term of antifungal agent productivity. The effects of initial pH, incubation temperature and agitation on biomass production by S. commune were investigated under submerged shake culture. In this work, it was found that the synthetic medium with initial solution pH of 6.5 and incubated at 30ºC with shaking at 150 rpm provided the highest biomass production. The biomass extract from S. commune was then applied onto the rubberwood block panel to investigate its effectiveness. The results showed that biomass extract at a concentration of 5 μg/μL could inhibit the growth of selected rubberwood-degrading fungi, such as Lentinus sp., L. strigosus and Pycnoporus sanguineus

Instantaneous Biosynthesis of Silver Nanoparticles by Selected Macro Fungi

Abstract: Silver nanoparticles (AgNPs) have been reported to be useful in various medical and life sciences related application. It has received considerable attention in order to develop an efficient methodology for its synthesis. Conventional chemical synthesis is said to be harmful when used in medical related applications. Hence, this study was carried out with locally isolated fungi to synthesize silver nanoparticles biologically. Three modes of biosynthesis were identified: biosynthesis with the mycelia for extracellular and intracellular cells synthesis and sans mycelia for culture free synthesis. The white rot fungus, Schizophyllum commune, was found to have an ability to produce AgNPs instantaneously. The biosynthesis of AgNPs was further confirmed with gas chromatography-mass spectroscopy (GC-MS), UV-visible spectroscopy, and Zetasizer Nano ZS

MEDIA SELECTION FOR MYCELIA GROWTH, ANTIFUNGAL ACTIVITY AGAINST WOOD-DEGRADING FUNGI, AND GC-MS STUDY BY PYCNOPORUS SANGUINEUS

Wood-decaying fungi present a serious threat to items made from rubberwood (Hevea brasiliensis). Though conventional chemical control has been a successful method for preserving wood against stain and decay fungi growth, the effects of these chemicals are of concern because they create problems for the environment and public health. Pycnoporus sanguineus (P. sanguineus), is a white-rot fungus that invades wood during its growth, storage, or use, causing decay or other property changes. It was considered in this work as a potential source of bioactive compounds and investigated for its natural antifungal activity using a minimum inhibitory concentration assay against wood-degrading fungi. It was found that media consisting of 10.0 g/L malt extract, yeast extract, dextrose, and maltose, respectively at pH 4.7±0.2 provided the highest biomass production by P. sanguineus. Results showed that the antifungal properties of methanol and water extract of P. sanguineus mycelia and supernatant ranged from MIC values of 0.1 to 5.0 µg/µL. 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP) was found to be the major component in the extract of this fungus, based on analysis using gas chromatography - mass spectrometry

Instantaneous Biosynthesis of Silver Nanoparticles by Selected Macro Fungi

Abstract: Silver nanoparticles (AgNPs) have been reported to be useful in various medical and life sciences related application. Therefore, the synthesis of AgNPs has received considerable attention in order to develop efficient methodology for its synthesis. Conventional chemical synthesis is said to be harmful when used in medical related applications. Hence, current research involving biological synthesis of AgNPs is performed. Several locally isolated fungi were screened and we discovered silver nanoparticles (AgNPs) were produced after treating silver nitrate with fungus mycelia/culture supernatant. Three modes of biosynthesis were identified: with the mycelia, extracellular and intracellular biosynthesis is possible and thirdly though the culture supernatant sans mycelia. From this research, we also confirmed that Schizophyllum commune has the ability to produce AgNPs instantaneously. The biosynthesis of AgNPs is further confirmed with gas chromatography-mass spectroscopy (GC-MS), UV-visible spectroscopy, and Zetasizer Nano ZS