Application of Allogenic Adipose-Derived Multipotent Mesenchymal Stromal Cells from Cat for Tibial Bone Pseudoarthrosis Therapy (Case Report)

© 2016, Springer Science+Business Media New York.A clinical case reported here demonstrates the possibility of using the multipotent mesenchymal stromal cells obtained from the donor animal to stimulate the process of bone formation in surgical treatment of tibia pseudoarthrosis in the cat. The cells were isolated from adipose tissue of the donor cat and express on their surface main markers of mesenchymal stromal cells—CD 44 and Thy-1. As a result of manipulation, the bone regenerate was formed and the support function of the injured limb was restored

Considerations and strategies in L2 vocabulary acquisition among Japanese 1st year university students.

This article proposes a framework of strategies1） for L2 vocabulary acquisition among low-to-mid level L2 learners in their first year at Kansai University. The framework relies upon considerations posited by Kudo (1999), as well as, Hunt and Beglar’s (2005) model for developing EFL reading vocabulary, although the objective for our target learners is tofacilitate vocabulary acquisition in a learner-centered communicative context, where possible. This paper firstly underlines the critical role of vocabulary in second language acquisition, while raising awareness of the surrounding pedagogic climate in Japanese secondary education.研究ノー

Adipose-Derived Mesenchymal Stem Cells Applied in Fibrin Glue Stimulate Peripheral Nerve Regeneration

Mesenchymal stem cells (MSCs) hold a great promise for cell therapy. To date, they represent one of the best choices for the treatment of post-traumatic injuries of the peripheral nervous system. Although autologous can be easily transplanted in the injured area, clinical advances in this filed have been impaired by lack of preservation of graft cells into the injury area after transplantation. Indeed, cell viability is not retained after injection into the blood stream, and cells injected directly into the area of injury either are washed off or inhibit regeneration through scar formation and neuroma development. This study proposes a new way of MSCs delivery to the area of traumatic injury by using fibrin glue, which not only fixes cells at the site of application but also provides extracellular matrix support. Using a sciatic nerve injury model, MSC derived from adipose tissue embedded in fibrin glue were able to enter the nerve and migrate mainly retrogradely after transplantation. They also demonstrated a neuroprotective effect on DRG L5 sensory neurons and stimulated axon growth and myelination. Post-traumatic changes of the sensory neuron phenotype were also improved. Importantly, MSCs stimulated nerve angiogenesis and motor function recovery. Therefore, our data suggest that MSC therapy using fibrin glue is a safe and efficient method of cell transplantation in cases of sciatic nerve injury, and that this method of delivery of regeneration stimulants could be beneficial for the successful treatment of other central and peripheral nervous system conditions

Genetic modification of adipose derived stem cells with recombinant plasmid DNA pBud-VEGF-FGF2 results in increased of IL-8 and MCP-1 secretion

Bacterial plasmid DNA is often used in gene therapy application as a vector for delivering recombinant genes into eukaryotic cells. In this work we study effect of plasmid DNA encoding pro-angiogenic growth factors VEGF and FGF2 on cytokine production of human adipose derived stem cells (ADSC). Adipose tissue contain progenitor and stem cells with angiogenic potential which are considered promising cell source for treating ischemic conditions. Genetic modification of human ADSC with recombinant plasmids encoding therapeutic growth factors might change therapeutic potential of these cells. We report that genetic modification of ADSC with recombinant plasmid pBud-VEGF-FGF2 results in expected increased of VEGF secretion and also results in increase of IL-8 and MCP-1 secretion into culture medium

Multiplex Analysis of the Activation of the Immune System after Transection of the Rat’s Sciatic Nerve

© 2016, Springer Science+Business Media New York.In this study, using the model of sciatic nerve transection in rats, the cytokine profile of peripheral blood serum and rat sciatic nerve fragments was measured 3, 7 and 14 days after the injury. Histological analysis of sciatic nerve longitudinal sections was carried out at the same periods of time. The cytokine profile of peripheral blood serum of an intact rat as well as the cytokine profile of an intact homogenized sciatic nerve of a rat was examined. Comparative study of changes in quantitative measures before and after the injury was carried out. It was shown that in peripheral blood serum after the sciatic nerve transection, there was a change in quantitative measures of IL­1a, Leptin, IL6, MCP1, and MIP­2. In the samples of the rat sciatic nerve, the changes were found in the levels of EGF, Fractalkine, G­CSF, IFNg, IL10, IL17A, IL18, IL2, IL6, IP10, Leptin, LIX, MCP1, MIP­1a, MIP­2, and RANTES

Use of Gene-Activated Demineralized Bone Allograft in the Therapy of Ulnar Pseudarthrosis. Case Report

© 2016, Springer Science+Business Media New York.This paper presents a clinical case of successful ulnar pseudarthrosis treatment using a gene-activated bone allograft containing VEGF (vascular endothelial growth factor) and BMP2 (bone morphogenetic protein 2) in the form of a multicystron plasmid. Demineralized bone matrix with applied recombinant plasmid DNA was grafted into the bone defect using the classical open surgical approach. Two months after the surgery, the patient noticed the disappearance of pain including pain during activity. On X-rays of ulna, signs of union in the form of callus formation were found

Stimulation of rat's sciatic nerve post-traumatic regeneration using plasmids expressing vascular endothelial growth factor and basic fibroblast growth factor

The development of effective treatments for patients with peripheral nerve injury is an urgent task of biomedicine. «Gold» standard in restoring the integrity of nerve conduits is auto-nerve transplantation in which a peripheral nerve defect is corrected with autologous nerve graft. Here we propose a method for stimulating revascularization and regeneration of auto-nerve graft by a local injection of plasmid pBud-VEGFFGF2, expressing vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF2). It is shown that direct injection of plasmid pBud-VEGF-FGF2 in the proximal and distal segments of nerve, as well as in the auto-nerve graft, stimulates the regeneration of the rat's sciatic nerve and restores motor activity